Capsid scaffolding protein

Details

Name
Capsid scaffolding protein
Synonyms
  • Capsid protein P40
  • pPR
  • Protease precursor
  • Protein EC-RF3/EC-RF3A
  • Virion structural protein BVRF2
Gene Name
Not Available
Organism
HHV-4
Amino acid sequence
>lcl|BSEQ0003881|Capsid scaffolding protein
MVQAPSVYVCGFVERPDAPPKDACLHLDPLTVKSQLPLKKPLPLTVEHLPDAPVGSVFGL
YQSRAGLFSAASITSGDFLSLLDSIYHDCDIAQSQRLPLPREPKVEALHAWLPSLSLASL
HPDIPQTTADGGKLSFFDHVSICALGRRRGTTAVYGTDLAWVLKHFSDLEPSIAAQIEND
ANAAKRESGCPEDHPLPLTKLIAKAIDAGFLRNRVETLRQDRGVANIPAESYLKASDAPD
LQKPDKALQSPPPASTDPATMLSGNAGEGATACGGSAAAGQDLISVPRNTFMTLLQTNLD
NKPPRQTPLPYAAPLPPFSHQAIATAPSYGPGAGAVAPAGGYFTSPGGYYAGPAGGDPGA
FLAMDAHTYHPHPHPPPAYFGLPGLFGPPPPVPPYYGSHLRADYVPAPSRSNKRKRDPEE
DEEGGGLFPGEDATLYRKDIAGLSKSVNELQHTLQALRRETLSYGHTGVGYCPQQGPCYT
HSGPYGFQPHQSYEVPRYVPHPPPPPTSHQAAQAQPPPPGTQAPEAHCVAESTIPEAGAA
GNSGPREDTNPQQPTTEGHHRGKKLVQASASGVAQSKEPTTPKAKSVSAHLKSIFCEELL
NKRVA
Number of residues
605
Molecular Weight
64101.37
Theoretical pI
6.79
GO Classification
Functions
serine-type endopeptidase activity
Processes
viral release from host cell
Components
host cell cytoplasm / host cell nucleus
General Function
Serine-type endopeptidase activity
Specific Function
Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein VP5 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein VP5. Cleavages products are evicted from the capsid before or during DNA packaging.Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-|-Ser-, -Ala-|-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein VP5. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Host cytoplasm
Gene sequence
>lcl|BSEQ0021311|Capsid scaffolding protein
ATGCTATCAGGTAACGCAGGAGAAGGAGCAACAGCCTGCGGAGGTTCGGCCGCCGCGGGC
CAGGACCTCATCAGCGTCCCCCGCAACACCTTTATGACACTGCTTCAGACCAACCTGGAC
AACAAACCGCCGAGGCAGACCCCGCTACCCTACGCGGCCCCGCTGCCCCCCTTTTCCCAC
CAGGCAATAGCCACCGCGCCTTCCTACGGTCCTGGGGCCGGAGCGGTCGCCCCGGCCGGC
GGCTACTTTACCTCCCCAGGAGGTTACTACGCCGGGCCCGCGGGCGGGGACCCGGGTGCC
TTCTTGGCGATGGACGCTCACACCTACCACCCCCACCCACACCCCCCTCCGGCCTACTTT
GGCTTGCCGGGCCTCTTTGGCCCCCCTCCACCCGTGCCTCCTTACTACGGATCCCACTTG
CGGGCAGACTACGTCCCCGCTCCCTCGCGATCCAACAAGCGGAAAAGAGACCCCGAGGAG
GATGAAGAAGGCGGGGGGCTATTCCCGGGGGAGGACGCCACCCTCTACCGCAAGGACATA
GCGGGCCTCTCCAAGAGTGTGAATGAGTTACAGCACACGCTACAGGCCCTGCGCCGGGAG
ACGCTGTCCTACGGCCACACCGGAGTCGGATACTGCCCCCAGCAGGGCCCCTGCTACACC
CACTCGGGGCCTTACGGATTTCAGCCTCATCAAAGCTACGAAGTGCCCAGATACGTCCCT
CATCCGCCCCCACCACCAACTTCTCACCAGGCAGCTCAGGCGCAGCCTCCACCCCCGGGC
ACACAGGCCCCCGAAGCCCACTGTGTGGCCGAGTCCACGATCCCTGAGGCGGGAGCAGCC
GGGAACTCTGGACCCCGGGAGGACACCAACCCTCAGCAGCCCACCACCGAGGGCCACCAC
CGCGGAAAGAAACTGGTGCAGGCCTCTGCGTCCGGAGTGGCTCAGTCTAAGGAGCCCACC
ACCCCCAAGGCCAAGTCTGTGTCAGCCCACCTCAAGTCCATCTTTTGCGAGGAATTGCTG
AATAAACGCGTGGCTTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP03234
UniProtKB Entry NameSCAF_EBVB9
GenBank Protein ID1334909
GenBank Gene IDV01555
General References
  1. Bankier AT, Deininger PL, Farrell PJ, Barrell BG: Sequence analysis of the 17,166 base-pair EcoRI fragment C of B95-8 Epstein-Barr virus. Mol Biol Med. 1983 Jul;1(1):21-45. [Article]
  2. Baer R, Bankier AT, Biggin MD, Deininger PL, Farrell PJ, Gibson TJ, Hatfull G, Hudson GS, Satchwell SC, Seguin C, et al.: DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature. 1984 Jul 19-25;310(5974):207-11. [Article]
  3. Henson BW, Perkins EM, Cothran JE, Desai P: Self-assembly of Epstein-Barr virus capsids. J Virol. 2009 Apr;83(8):3877-90. doi: 10.1128/JVI.01733-08. Epub 2009 Jan 21. [Article]
  4. Buisson M, Hernandez JF, Lascoux D, Schoehn G, Forest E, Arlaud G, Seigneurin JM, Ruigrok RW, Burmeister WP: The crystal structure of the Epstein-Barr virus protease shows rearrangement of the processed C terminus. J Mol Biol. 2002 Nov 15;324(1):89-103. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03976PhosphorylisopropaneexperimentalunknownDetails