Penton protein
Details
- Name
- Penton protein
- Synonyms
- CP-P
- Penton base protein
- Protein III
- Gene Name
- Not Available
- Organism
- HAdV-2
- Amino acid sequence
>lcl|BSEQ0011113|Penton protein MQRAAMYEEGPPPSYESVVSAAPVAAALGSPFDAPLDPPFVPPRYLRPTGGRNSIRYSEL APLFDTTRVYLVDNKSTDVASLNYQNDHSNFLTTVIQNNDYSPGEASTQTINLDDRSHWG GDLKTILHTNMPNVNEFMFTNKFKARVMVSRSLTKDKQVELKYEWVEFTLPEGNYSETMT IDLMNNAIVEHYLKVGRQNGVLESDIGVKFDTRNFRLGFDPVTGLVMPGVYTNEAFHPDI ILLPGCGVDFTHSRLSNLLGIRKRQPFQEGFRITYDDLEGGNIPALLDVDAYQASLKDDT EQGGDGAGGGNNSGSGAEENSNAAAAAMQPVEDMNDHAIRGDTFATRAEEKRAEAEAAAE AAAPAAQPEVEKPQKKPVIKPLTEDSKKRSYNLISNDSTFTQYRSWYLAYNYGDPQTGIR SWTLLCTPDVTCGSEQVYWSLPDMMQDPVTFRSTSQISNFPVVGAELLPVHSKSFYNDQA VYSQLIRQFTSLTHVFNRFPENQILARPPAPTITTVSENVPALTDHGTLPLRNSIGGVQR VTITDARRRTCPYVYKALGIVSPRVLSSRTF
- Number of residues
- 571
- Molecular Weight
- 63254.24
- Theoretical pI
- 5.07
- GO Classification
- Functionsstructural molecule activityProcessesclathrin-mediated endocytosis of virus by host cell / virion attachment to host cellComponentshost cell nucleus / T=25 icosahedral viral capsid
- General Function
- Structural molecule activity
- Specific Function
- Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.
- Pfam Domain Function
- Adeno_Penton_B (PF01686)
- Transmembrane Regions
- Not Available
- Cellular Location
- Virion
- Gene sequence
>lcl|BSEQ0011114|Penton protein ATGCAGCGCGCGGCGATGTATGAGGAAGGTCCTCCTCCCTCCTACGAGAGCGTGGTGAGC GCGGCGCCAGTGGCGGCGGCGCTGGGTTCCCCCTTCGATGCTCCCCTGGACCCGCCGTTT GTGCCTCCGCGGTACCTGCGGCCTACCGGGGGGAGAAACAGCATCCGTTACTCTGAGTTG GCACCCCTATTCGACACCACCCGTGTGTACCTTGTGGACAACAAGTCAACGGATGTGGCA TCCCTGAACTACCAGAACGACCACAGCAACTTTCTAACCACGGTCATTCAAAACAATGAC TACAGCCCGGGGGAGGCAAGCACACAGACCATCAATCTTGACGACCGTTCGCACTGGGGC GGCGACCTGAAAACCATCCTGCATACCAACATGCCAAATGTGAACGAGTTCATGTTTACC AATAAGTTTAAGGCGCGGGTGATGGTGTCGCGCTCGCTTACTAAGGACAAACAGGTGGAG CTGAAATATGAGTGGGTGGAGTTCACGCTGCCCGAGGGCAACTACTCCGAGACCATGACC ATAGACCTTATGAACAACGCGATCGTGGAGCACTACTTGAAAGTGGGCAGGCAGAACGGG GTTCTGGAAAGCGACATCGGGGTAAAGTTTGACACCCGCAACTTCAGACTGGGGTTTGAC CCAGTCACTGGTCTTGTCATGCCTGGGGTATATACAAACGAAGCCTTCCATCCAGACATC ATTTTGCTGCCAGGATGCGGGGTGGACTTCACCCACAGCCGCCTGAGCAACTTGTTGGGC ATCCGCAAGCGGCAACCCTTCCAGGAGGGCTTTAGGATCACCTACGATGACCTGGAGGGT GGTAACATTCCCGCACTGTTGGATGTGGACGCCTACCAGGCAAGCTTAAAAGATGACACC GAACAGGGCGGGGATGGCGCAGGCGGCGGCAACAACAGTGGCAGCGGCGCGGAAGAGAAC TCCAACGCGGCAGCCGCGGCAATGCAGCCGGTGGAGGACATGAACGATCATGCCATTCGC GGCGACACCTTTGCCACACGGGCGGAGGAGAAGCGCGCTGAGGCCGAGGCAGCGGCAGAA GCTGCCGCCCCCGCTGCGCAACCCGAGGTCGAGAAGCCTCAGAAGAAACCGGTGATCAAA CCCCTGACAGAGGACAGCAAGAAACGCAGTTACAACCTAATAAGCAATGACAGCACCTTC ACCCAGTACCGCAGCTGGTACCTTGCATACAACTACGGCGACCCTCAGACCGGGATCCGC TCATGGACCCTCCTTTGCACTCCTGACGTAACCTGCGGCTCGGAGCAGGTCTACTGGTCG TTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGAGCCAGATCAGCAACTTT CCGGTGGTGGGCGCCGAGCTGTTGCCCGTGCACTCCAAGAGCTTCTACAACGACCAGGCC GTCTACTCCCAGCTCATCCGCCAGTTTACCTCTCTGACCCACGTGTTCAATCGCTTTCCC GAGAACCAGATTTTGGCGCGCCCGCCAGCCCCCACCATCACCACCGTCAGTGAAAACGTT CCTGCTCTCACAGATCACGGGACGCTACCGCTGCGCAACAGCATCGGAGGAGTCCAGCGA GTGACCATTACTGACGCCAGACGCCGCACCTGCCCCTACGTTTACAAGGCCCTGGGCATA GTCTCGCCGCGCGTCCTATCGAGCCGCACTTTTTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P03276 UniProtKB Entry Name CAPSP_ADE02 GenBank Protein ID 209827 GenBank Gene ID J01917 - General References
- Roberts RJ, O'Neill KE, Yen CT: DNA sequences from the adenovirus 2 genome. J Biol Chem. 1984 Nov 25;259(22):13968-75. [Article]
- Alestrom P, Akusjarvi G, Lager M, Yeh-kai L, Pettersson U: Genes encoding the core proteins of adenovirus type 2. J Biol Chem. 1984 Nov 25;259(22):13980-5. [Article]
- Bergstrom Lind S, Artemenko KA, Elfineh L, Zhao Y, Bergquist J, Pettersson U: The phosphoproteome of the adenovirus type 2 virion. Virology. 2012 Nov 10;433(1):253-61. doi: 10.1016/j.virol.2012.08.012. Epub 2012 Aug 28. [Article]
- van Raaij MJ, Mitraki A, Lavigne G, Cusack S: A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein. Nature. 1999 Oct 28;401(6756):935-8. [Article]
- Galinier R, Gout E, Lortat-Jacob H, Wood J, Chroboczek J: Adenovirus protein involved in virus internalization recruits ubiquitin-protein ligases. Biochemistry. 2002 Dec 3;41(48):14299-305. [Article]
- Meier O, Boucke K, Hammer SV, Keller S, Stidwill RP, Hemmi S, Greber UF: Adenovirus triggers macropinocytosis and endosomal leakage together with its clathrin-mediated uptake. J Cell Biol. 2002 Sep 16;158(6):1119-31. Epub 2002 Sep 9. [Article]
- Lyle C, McCormick F: Integrin alphavbeta5 is a primary receptor for adenovirus in CAR-negative cells. Virol J. 2010 Jul 8;7:148. doi: 10.1186/1743-422X-7-148. [Article]
- San Martin C: Latest insights on adenovirus structure and assembly. Viruses. 2012 May;4(5):847-77. doi: 10.3390/v4050847. Epub 2012 May 21. [Article]
- Liu H, Jin L, Koh SB, Atanasov I, Schein S, Wu L, Zhou ZH: Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks. Science. 2010 Aug 27;329(5995):1038-43. doi: 10.1126/science.1187433. [Article]
- Zubieta C, Schoehn G, Chroboczek J, Cusack S: The structure of the human adenovirus 2 penton. Mol Cell. 2005 Jan 7;17(1):121-35. [Article]
- Fabry CM, Rosa-Calatrava M, Conway JF, Zubieta C, Cusack S, Ruigrok RW, Schoehn G: A quasi-atomic model of human adenovirus type 5 capsid. EMBO J. 2005 May 4;24(9):1645-54. Epub 2005 Apr 21. [Article]
- Zubieta C, Blanchoin L, Cusack S: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera. FEBS J. 2006 Sep;273(18):4336-45. [Article]