Type-2 restriction enzyme EcoRV
Details
- Name
- Type-2 restriction enzyme EcoRV
- Synonyms
- 3.1.21.4
- Endonuclease EcoRV
- R.EcoRV
- Type II restriction enzyme EcoRV
- Gene Name
- ecoRVR
- Organism
- Escherichia coli
- Amino acid sequence
>lcl|BSEQ0017421|Type-2 restriction enzyme EcoRV MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKIAEKHG YIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIKFTLGGYTSFIRNNTKN IVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPYKGVKVFLQDKWVIAGDL AGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWI YRGRK
- Number of residues
- 245
- Molecular Weight
- 28650.14
- Theoretical pI
- 9.28
- GO Classification
- FunctionsDNA binding / metal ion binding / Type II site-specific deoxyribonuclease activityProcessesDNA restriction-modification system
- General Function
- Type ii site-specific deoxyribonuclease activity
- Specific Function
- Recognizes the double-stranded sequence GATATC and cleaves after T-3.
- Pfam Domain Function
- Endonuc-EcoRV (PF09233)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0017422|Type-2 restriction enzyme EcoRV (ecoRVR) ATGAGTCTTCGTTCTGATTTAATTAATGCACTATATGATGAAAATCAAAAATATGATGTA TGCGGAATAATATCTGCAGAAGGAAAAATATACCCATTGGGAAGTGACACAAAAGTTCTA AGCACAATATTTGAGTTATTCTCAAGACCAATAATAAATAAAATAGCAGAAAAACATGGG TATATTGTAGAAGAACCTAAACAACAAAATCATTATCCTGACTTTACTCTTTACAAACCA AGCGAACCAAATAAAAAAATTGCAATAGATATAAAAACAACATATACAAACAAAGAAAAC GAAAAAATCAAGTTCACTCTTGGTGGGTATACCAGCTTTATACGAAACAACACAAAAAAT ATTGTTTATCCATTTGACCAATATATCGCCCATTGGATAATCGGATATGTATATACAAGA GTTGCTACAAGAAAATCATCTTTAAAAACATATAATATAAATGAACTCAATGAAATCCCT AAACCATACAAAGGCGTAAAGGTTTTCTTACAAGATAAATGGGTTATTGCTGGAGATTTG GCAGGATCTGGAAACACAACAAATATAGGTAGCATTCATGCCCACTATAAAGACTTTGTA GAAGGAAAAGGAATATTTGACTCAGAGGATGAGTTTTTAGACTATTGGAGAAATTATGAA AGAACCAGTCAATTAAGAAATGACAAGTATAATAATATAAGCGAATACAGAAACTGGATA TACCGAGGAAGAAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P04390 UniProtKB Entry Name T2E5_ECOLX GenBank Protein ID 41325 GenBank Gene ID X00530 - General References
- Bougueleret L, Schwarzstein M, Tsugita A, Zabeau M: Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli. Nucleic Acids Res. 1984 Apr 25;12(8):3659-76. [Article]
- Thielking V, Selent U, Kohler E, Wolfes H, Pieper U, Geiger R, Urbanke C, Winkler FK, Pingoud A: Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry. 1991 Jul 2;30(26):6416-22. [Article]
- Winkler FK, Banner DW, Oefner C, Tsernoglou D, Brown RS, Heathman SP, Bryan RK, Martin PD, Petratos K, Wilson KS: The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments. EMBO J. 1993 May;12(5):1781-95. [Article]
- Kostrewa D, Winkler FK: Mg2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution. Biochemistry. 1995 Jan 17;34(2):683-96. [Article]
- Perona JJ, Martin AM: Conformational transitions and structural deformability of EcoRV endonuclease revealed by crystallographic analysis. J Mol Biol. 1997 Oct 17;273(1):207-25. [Article]
- Horton NC, Perona JJ: Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts. J Biol Chem. 1998 Aug 21;273(34):21721-9. [Article]
- Horton NC, Perona JJ: Role of protein-induced bending in the specificity of DNA recognition: crystal structure of EcoRV endonuclease complexed with d(AAAGAT) + d(ATCTT). J Mol Biol. 1998 Apr 10;277(4):779-87. [Article]
- Thomas MP, Brady RL, Halford SE, Sessions RB, Baldwin GS: Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group. Nucleic Acids Res. 1999 Sep 1;27(17):3438-45. [Article]