Complement decay-accelerating factor


Complement decay-accelerating factor
  • CR
  • DAF
Gene Name
Amino acid sequence
>lcl|BSEQ0001850|Complement decay-accelerating factor
Number of residues
Molecular Weight
Theoretical pI
GO Classification
lipid binding / virus receptor activity
CD4-positive, alpha-beta T cell cytokine production / complement activation, classical pathway / innate immune response / negative regulation of complement activation / positive regulation of CD4-positive, alpha-beta T cell activation / positive regulation of CD4-positive, alpha-beta T cell proliferation / positive regulation of cytosolic calcium ion concentration / regulation of complement activation / regulation of lipopolysaccharide-mediated signaling pathway / respiratory burst / viral entry into host cell
anchored component of membrane / cell surface / cytosol / extracellular exosome / extracellular region / integral component of plasma membrane / membrane raft / plasma membrane
General Function
Virus receptor activity
Specific Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.(Microbial infection) Acts as a receptor for coxsackievirus A21, coxsackieviruses B1, B3 and B5 (PubMed:9151867). Acts as a receptor for human enterovirus 70 and D68 (Probable) (PubMed:8764022). Acts as a receptor for human echoviruses 6, 7, 11, 12, 20 and 21 (PubMed:7525274).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cell membrane
Gene sequence
>lcl|BSEQ0019031|Complement decay-accelerating factor (CD55)
Chromosome Location
External Identifiers
UniProtKB IDP08174
UniProtKB Entry NameDAF_HUMAN
GenBank Protein ID181468
GenBank Gene IDM31516
GenAtlas IDCD55
General References
  1. Caras IW, Davitz MA, Rhee L, Weddell G, Martin DW Jr, Nussenzweig V: Cloning of decay-accelerating factor suggests novel use of splicing to generate two proteins. Nature. 1987 Feb 5-11;325(6104):545-9. [Article]
  2. Osuka F, Endo Y, Higuchi M, Suzuki H, Shio Y, Fujiu K, Kanno R, Oishi A, Terashima M, Fujita T, Gotoh M: Molecular cloning and characterization of novel splicing variants of human decay-accelerating factor. Genomics. 2006 Sep;88(3):316-22. Epub 2006 Feb 28. [Article]
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  5. Ewulonu UK, Ravi L, Medof ME: Characterization of the decay-accelerating factor gene promoter region. Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4675-9. [Article]
  6. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML: Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement. Proc Natl Acad Sci U S A. 1987 Apr;84(7):2007-11. [Article]
  7. Sugita Y, Negoro T, Matsuda T, Sakamoto T, Tomita M: Improved method for the isolation and preliminary characterization of human DAF (decay-accelerating factor). J Biochem. 1986 Jul;100(1):143-50. [Article]
  8. Nakano Y, Sugita Y, Ishikawa Y, Choi NH, Tobe T, Tomita M: Isolation of two forms of decay-accelerating factor (DAF) from human urine. Biochim Biophys Acta. 1991 Jul 8;1074(2):326-30. [Article]
  9. Moran P, Raab H, Kohr WJ, Caras IW: Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site. J Biol Chem. 1991 Jan 15;266(2):1250-7. [Article]
  10. Nakano Y, Sumida K, Kikuta N, Miura NH, Tobe T, Tomita M: Complete determination of disulfide bonds localized within the short consensus repeat units of decay accelerating factor (CD55 antigen). Biochim Biophys Acta. 1992 Jun 12;1116(3):235-40. [Article]
  11. Ward T, Pipkin PA, Clarkson NA, Stone DM, Minor PD, Almond JW: Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method. EMBO J. 1994 Nov 1;13(21):5070-4. [Article]
  12. Bergelson JM, Chan M, Solomon KR, St John NF, Lin H, Finberg RW: Decay-accelerating factor (CD55), a glycosylphosphatidylinositol-anchored complement regulatory protein, is a receptor for several echoviruses. Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6245-8. [Article]
  13. Shafren DR, Bates RC, Agrez MV, Herd RL, Burns GF, Barry RD: Coxsackieviruses B1, B3, and B5 use decay accelerating factor as a receptor for cell attachment. J Virol. 1995 Jun;69(6):3873-7. [Article]
  14. Karnauchow TM, Tolson DL, Harrison BA, Altman E, Lublin DM, Dimock K: The HeLa cell receptor for enterovirus 70 is decay-accelerating factor (CD55). J Virol. 1996 Aug;70(8):5143-52. [Article]
  15. Shafren DR, Dorahy DJ, Ingham RA, Burns GF, Barry RD: Coxsackievirus A21 binds to decay-accelerating factor but requires intercellular adhesion molecule 1 for cell entry. J Virol. 1997 Jun;71(6):4736-43. [Article]
  16. Blomqvist S, Savolainen C, Raman L, Roivainen M, Hovi T: Human rhinovirus 87 and enterovirus 68 represent a unique serotype with rhinovirus and enterovirus features. J Clin Microbiol. 2002 Nov;40(11):4218-23. [Article]
  17. Elortza F, Nuhse TS, Foster LJ, Stensballe A, Peck SC, Jensen ON: Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins. Mol Cell Proteomics. 2003 Dec;2(12):1261-70. Epub 2003 Sep 29. [Article]
  18. Elortza F, Mohammed S, Bunkenborg J, Foster LJ, Nuhse TS, Brodbeck U, Peck SC, Jensen ON: Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment. J Proteome Res. 2006 Apr;5(4):935-43. [Article]
  19. Hafenstein S, Bowman VD, Chipman PR, Bator Kelly CM, Lin F, Medof ME, Rossmann MG: Interaction of decay-accelerating factor with coxsackievirus B3. J Virol. 2007 Dec;81(23):12927-35. Epub 2007 Sep 5. [Article]
  20. Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res. 2009 Feb;8(2):651-61. doi: 10.1021/pr8008012. [Article]
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  22. Mizuhashi K, Chaya T, Kanamoto T, Omori Y, Furukawa T: Obif, a Transmembrane Protein, Is Required for Bone Mineralization and Spermatogenesis in Mice. PLoS One. 2015 Jul 24;10(7):e0133704. doi: 10.1371/journal.pone.0133704. eCollection 2015. [Article]
  23. Lublin DM, Mallinson G, Poole J, Reid ME, Thompson ES, Ferdman BR, Telen MJ, Anstee DJ, Tanner MJ: Molecular basis of reduced or absent expression of decay-accelerating factor in Cromer blood group phenotypes. Blood. 1994 Aug 15;84(4):1276-82. [Article]
  24. Storry JR, Sausais L, Hue-Roye K, Mudiwa F, Ferrer Z, Blajchman MA, Lublin DM, Ma BW, Miquel JF, Nervi F, Pereira J, Reid ME: GUTI: a new antigen in the Cromer blood group system. Transfusion. 2003 Mar;43(3):340-4. [Article]
  25. Reid ME, Mallinson G, Sim RB, Poole J, Pausch V, Merry AH, Liew YW, Tanner MJ: Biochemical studies on red blood cells from a patient with the Inab phenotype (decay-accelerating factor deficiency). Blood. 1991 Dec 15;78(12):3291-7. [Article]
  26. Williams P, Chaudhry Y, Goodfellow IG, Billington J, Powell R, Spiller OB, Evans DJ, Lea S: Mapping CD55 function. The structure of two pathogen-binding domains at 1.7 A. J Biol Chem. 2003 Mar 21;278(12):10691-6. Epub 2002 Dec 22. [Article]
  27. Lukacik P, Roversi P, White J, Esser D, Smith GP, Billington J, Williams PA, Rudd PM, Wormald MR, Harvey DJ, Crispin MD, Radcliffe CM, Dwek RA, Evans DJ, Morgan BP, Smith RA, Lea SM: Complement regulation at the molecular level: the structure of decay-accelerating factor. Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1279-84. Epub 2004 Jan 20. [Article]
  28. Uhrinova S, Lin F, Ball G, Bromek K, Uhrin D, Medof ME, Barlow PN: Solution structure of a functionally active fragment of decay-accelerating factor. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4718-23. Epub 2003 Apr 2. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB00446Chloramphenicolapproved, vet_approvedunknownotherDetails