Parathion hydrolase
Details
- Name
- Parathion hydrolase
- Synonyms
- 3.1.8.1
- Phosphotriesterase
- PTE
- Gene Name
- opd
- UniProtKB Entry
- P0A433Swiss-Prot
- Organism
- Sphingobium fuliginis (strain ATCC 27551)
- NCBI Taxonomy ID
- 336203
- Amino acid sequence
>lcl|BSEQ0011087|Parathion hydrolase MQTRRVVLKSAAAAGTLLGGLAGCASVAGSIGTGDRINTVRGPITISEAGFTLTHEHICG SSAGFLRAWPEFFGSRKALAEKAVRGLRRARAAGVRTIVDVSTFDIGRDVSLLAEVSRAA DVHIVAATGLWFDPPLSMRLRSVEELTQFFLREIQYGIEDTGIRAGIIKVATTGKATPFQ ELVLKAAARASLATGVPVTTHTAASQRDGEQQAAIFESEGLSPSRVCIGHSDDTDDLSYL TALAARGYLIGLDHIPHSAIGLEDNASASALLGIRSWQTRALLIKALIDQGYMKQILVSN DWLFGFSSYVTNIMDVMDRVNPDGMAFIPLRVIPFLREKGVPQETLAGITVTNPARFLSP TLRAS
- Number of residues
- 365
- Molecular Weight
- 39003.24
- Theoretical pI
- 8.48
- GO Classification
- Functionsaryldialkylphosphatase activity / zinc ion bindingProcessescatabolic processComponentsplasma membrane
- General Function
- Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate (By similarity).
- Specific Function
- aryldialkylphosphatase activity
- Pfam Domain Function
- PTE (PF02126)
- Signal Regions
- 1-29
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell membrane
- Gene sequence
>lcl|BSEQ0003047|1098 bp ATGCAAACGAGAAGGGTTGTGCTCAAGTCTGCGGCCGCCGCAGGAACTCTGCTCGGCGGC CTGGCTGGGTGCGCGAGCGTGGCTGGATCGATCGGCACAGGCGATCGGATCAATACCGTG CGCGGTCCTATCACAATCTCTGAAGCGGGTTTCACACTGACTCACGAGCACATCTGCGGC AGCTCGGCAGGATTCTTGCGTGCTTGGCCAGAGTTCTTCGGTAGCCGCAAAGCTCTAGCG GAAAAGGCTGTGAGAGGATTGCGCCGCGCCAGAGCGGCTGGCGTGCGAACGATTGTCGAT GTGTCGACTTTCGATATCGGTCGCGACGTCAGTTTATTGGCCGAGGTTTCGCGGGCTGCC GACGTTCATATCGTGGCGGCGACCGGCTTGTGGTTCGACCCGCCACTTTCGATGCGATTG AGGAGTGTAGAGGAACTCACACAGTTCTTCCTGCGTGAGATTCAATATGGCATCGAAGAC ACCGGAATTAGGGCGGGCATTATCAAGGTCGCGACCACAGGCAAGGCGACCCCCTTTCAG GAGTTAGTGTTAAAGGCGGCCGCCCGGGCCAGCTTGGCCACCGGTGTTCCGGTAACCACT CACACGGCAGCAAGTCAGCGCGATGGTGAGCAGCAGGCCGCCATTTTTGAGTCCGAAGGC TTGAGCCCCTCACGGGTTTGTATTGGTCACAGCGATGATACTGACGATTTGAGCTATCTC ACCGCCCTCGCTGCGCGCGGATACCTCATCGGTCTAGACCACATCCCGCACAGTGCGATT GGTCTAGAAGATAATGCGAGTGCATCAGCCCTCCTGGGCATCCGTTCGTGGCAAACACGG GCTCTCTTGATCAAGGCGCTCATCGACCAAGGCTACATGAAACAAATCCTCGTTTCGAAT GACTGGCTGTTCGGGTTTTCGAGCTATGTCACCAACATCATGGACGTGATGGATCGCGTG AACCCCGACGGGATGGCCTTCATTCCACTGAGAGTGATCCCATTCCTACGAGAGAAGGGC GTCCCACAGGAAACGCTGGCAGGCATCACTGTGACTAACCCGGCGCGGTTCTTGTCACCG ACCTTGCGGGCGTCATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0A433 UniProtKB Entry Name OPD_SPHSA GenBank Protein ID 148713 GenBank Gene ID M29593 PDB ID(s) 1P6B, 1P6C - General References
- Mulbry WW, Karns JS: Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein. J Bacteriol. 1989 Dec;171(12):6740-6. [Article]
- Hill CM, Li WS, Thoden JB, Holden HM, Raushel FM: Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site. J Am Chem Soc. 2003 Jul 30;125(30):8990-1. [Article]
Associated Data
- Bio-Entities
Bio-Entity Type Parathion hydrolase (Flavobacterium sp. (strain ATCC 27551)) protein primary- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Diisopropyl methylphosphonate experimental unknown target Details Diethyl 4-Methylbenzylphosphonate experimental unknown target Details Phenylethyl alcohol experimental unknown target Details (5r)-5-Amino-6-Hydroxyhexylcarbamic Acid experimental unknown target Details Triethyl phosphate experimental unknown target Details Lysine Nz-Carboxylic Acid experimental unknown target Details Ethyl dihydrogen phosphate experimental unknown target Details