30S ribosomal protein S13
Details
- Name
- 30S ribosomal protein S13
- Synonyms
- Not Available
- Gene Name
- rpsM
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0012584|30S ribosomal protein S13 MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEV AKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK
- Number of residues
- 118
- Molecular Weight
- 13099.245
- Theoretical pI
- 11.43
- GO Classification
- FunctionsrRNA binding / structural constituent of ribosome / tRNA bindingProcessestranslationComponentscytosol / cytosolic small ribosomal subunit
- General Function
- Trna binding
- Specific Function
- Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA.In the E.coli 70S ribosome in the initiation state (PubMed:12809609) was modeled to contact the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; bridge B1a is broken in the model with bound EF-G, while the protein-protein contacts between S13 and L5 in B1b change (PubMed:12809609). The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states (PubMed:16272117), contacting alternately S13 or S19. In the two 3.5 angstroms resolved ribosome structures (PubMed:12859903) the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder.Contacts the tRNAs in the A and P sites.The C-terminal tail plays a role in the affinity of the 30S P site for different tRNAs.
- Pfam Domain Function
- Ribosomal_S13 (PF00416)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0012585|30S ribosomal protein S13 (rpsM) GTGGCCCGTATAGCAGGCATTAACATTCCTGATCATAAGCATGCCGTAATCGCATTAACT TCGATTTATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATCGCT GAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTT GCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCATCAAGCGCCTG ATGGATCTTGGTTGCTATCGCGGTTTGCGTCATCGTCGTGGTCTCCCGGTTCGCGGTCAG CGTACCAAGACCAACGCACGTACCCGTAAGGGTCCGCGCAAACCGATCAAGAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0A7S9 UniProtKB Entry Name RS13_ECOLI GenBank Protein ID 581217 GenBank Gene ID X02543 - General References
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