Phospholipase A1

Details

Name
Phospholipase A1
Synonyms
  • 3.1.1.32
  • Detergent-resistant phospholipase A
  • DR-phospholipase A
  • OM PLA
  • OMPLA
  • Outer membrane phospholipase A
  • Phosphatidylcholine 1-acylhydrolase
Gene Name
pldA
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0019218|Phospholipase A1
MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQEHDNPFTLYPYDTNYL
IYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGILGPNSVLGASYTQKSWWQL
SNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGYNHDSNGRSDPTSRSWNRLYT
RLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLKIGYHLGDAVLSAKGQYNWNTGY
GGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGVMLNDLF
Number of residues
289
Molecular Weight
33162.93
Theoretical pI
4.96
GO Classification
Functions
1-acyl-2-lysophosphatidylserine acylhydrolase activity / calcium ion binding / phosphatidylcholine 1-acylhydrolase activity / phosphatidylserine 1-acylhydrolase activity / phospholipase A2 activity / protein homodimerization activity
Processes
lipid catabolic process
Components
cell outer membrane / integral component of cell outer membrane / intrinsic component of cell outer membrane
General Function
Protein homodimerization activity
Specific Function
Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.
Pfam Domain Function
Transmembrane Regions
53-65 85-99 106-118 129-148 151-164 174-186 189-198 217-223 226-234 242-250 256-265 275-286
Cellular Location
Cell outer membrane
Gene sequence
>lcl|BSEQ0019219|Phospholipase A1 (pldA)
ATGCGGACTCTGCAGGGCTGGTTGTTGCCGGTGTTTATGTTGCCTATGGCAGTATATGCA
CAAGAGGCAACGGTGAAAGAGGTGCATGACGCGCCAGCGGTGCGTGGCAGTATTATCGCC
AATATGCTGCAGGAGCATGACAATCCGTTCACGCTCTATCCTTATGACACCAACTACCTC
ATTTACACCCAAACCAGCGATCTGAATAAAGAAGCGATTGCCAGTTACGACTGGGCGGAA
AATGCGCGTAAGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGG
ATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTG
TCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTC
GGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTAT
AACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACT
CGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGT
AATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGC
TATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTAC
GGCGGCGCGGAGTTAGGCTTAAGTTACCCGATCACCAAACATGTGCGCCTTTATACTCAG
GTTTACAGCGGCTATGGCGAATCGCTCATCGACTATAACTTCAACCAGACCCGTGTCGGT
GTGGGGGTTATGCTAAACGATTTGTTTTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0A921
UniProtKB Entry NamePA1_ECOLI
GenBank Protein ID757840
GenBank Gene IDX02143
General References
  1. Homma H, Kobayashi T, Chiba N, Karasawa K, Mizushima H, Kudo I, Inoue K, Ikeda H, Sekiguchi M, Nojima S: The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. J Biochem. 1984 Dec;96(6):1655-64. [Article]
  2. Daniels DL, Plunkett G 3rd, Burland V, Blattner FR: Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771-8. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Dekker N, Merck K, Tommassen J, Verheij HM: In vitro folding of Escherichia coli outer-membrane phospholipase A. Eur J Biochem. 1995 Aug 15;232(1):214-9. [Article]
  6. de Geus P, Verheij HM, Riegman NH, Hoekstra WP, de Haas GH: The pro- and mature forms of the E. coli K-12 outer membrane phospholipase A are identical. EMBO J. 1984 Aug;3(8):1799-802. [Article]
  7. Irino N, Nakayama K, Nakayama H: The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation. Mol Gen Genet. 1986 Nov;205(2):298-304. [Article]
  8. Homma H, Chiba N, Kobayashi T, Kudo I, Inoue K, Ikeda H, Sekiguchi M, Nojima S: Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. J Biochem. 1984 Dec;96(6):1645-53. [Article]
  9. Brok RG, Brinkman E, van Boxtel R, Bekkers AC, Verheij HM, Tommassen J: Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A. J Bacteriol. 1994 Feb;176(3):861-70. [Article]
  10. Horrevoets AJ, Verheij HM, de Haas GH: Inactivation of Escherichia coli outer-membrane phospholipase A by the affinity label hexadecanesulfonyl fluoride. Evidence for an active-site serine. Eur J Biochem. 1991 May 23;198(1):247-53. [Article]
  11. Dekker N, Tommassen J, Lustig A, Rosenbusch JP, Verheij HM: Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A. J Biol Chem. 1997 Feb 7;272(6):3179-84. [Article]
  12. Dekker N, Tommassen J, Verheij HM: Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo. J Bacteriol. 1999 May;181(10):3281-3. [Article]
  13. Snijder HJ, Ubarretxena-Belandia I, Blaauw M, Kalk KH, Verheij HM, Egmond MR, Dekker N, Dijkstra BW: Structural evidence for dimerization-regulated activation of an integral membrane phospholipase. Nature. 1999 Oct 14;401(6754):717-21. [Article]
  14. Snijder HJ, Kingma RL, Kalk KH, Dekker N, Egmond MR, Dijkstra BW: Structural investigations of calcium binding and its role in activity and activation of outer membrane phospholipase A from Escherichia coli. J Mol Biol. 2001 Jun 1;309(2):477-89. [Article]
  15. Snijder HJ, Van Eerde JH, Kingma RL, Kalk KH, Dekker N, Egmond MR, Dijkstra BW: Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: function of the Asn-His interaction in the catalytic triad. Protein Sci. 2001 Oct;10(10):1962-9. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB036921-Hexadecanosulfonyl-O-L-SerineexperimentalunknownDetails