Fumarate reductase iron-sulfur subunit
Details
- Name
- Fumarate reductase iron-sulfur subunit
- Synonyms
- 1.3.5.1
- Gene Name
- frdB
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0020724|Fumarate reductase iron-sulfur subunit MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRM AICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPY IIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRY NEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIAT LKPR
- Number of residues
- 244
- Molecular Weight
- 27122.805
- Theoretical pI
- 6.49
- GO Classification
- Functions2 iron, 2 sulfur cluster binding / 3 iron, 4 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / electron carrier activity / iron-sulfur cluster binding / metal ion binding / succinate dehydrogenase (ubiquinone) activity / succinate dehydrogenase activityProcessesanaerobic respiration / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / fermentation / tricarboxylic acid cycleComponentscytosol / membrane / plasma membrane fumarate reductase complex
- General Function
- Succinate dehydrogenase activity
- Specific Function
- Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0020725|Fumarate reductase iron-sulfur subunit (frdB) ATGGCTGAGATGAAAAACCTGAAAATTGAGGTGGTGCGCTATAACCCGGAAGTCGATACC GCACCGCATAGCGCATTCTATGAAGTGCCTTATGACGCAACTACCTCATTACTGGATGCG CTGGGCTACATCAAAGACAACCTGGCACCGGACCTGAGCTACCGCTGGTCCTGCCGTATG GCGATTTGTGGTTCCTGCGGCATGATGGTTAACAACGTGCCAAAACTGGCATGTAAAACC TTCCTGCGTGATTACACCGACGGTATGAAGGTTGAAGCGTTAGCTAACTTCCCGATTGAA CGCGATCTGGTGGTCGATATGACCCACTTCATCGAAAGTCTGGAAGCGATCAAACCGTAC ATCATCGGCAACTCCCGCACCGCGGATCAGGGTACTAACATCCAGACCCCGGCGCAGATG GCGAAGTATCACCAGTTCTCCGGTTGCATCAACTGTGGTTTGTGCTACGCCGCGTGCCCG CAGTTTGGCCTGAACCCAGAGTTCATCGGTCCGGCTGCCATTACGCTGGCGCATCGTTAT AACGAAGATAGCCGCGACCACGGTAAGAAGGAGCGTATGGCGCAGTTGAACAGCCAGAAC GGCGTATGGAGCTGTACTTTCGTGGGCTACTGCTCCGAAGTCTGCCCGAAACACGTCGAT CCGGCTGCGGCCATTCAGCAGGGCAAAGTAGAAAGTTCGAAAGACTTTCTTATCGCGACC CTGAAACCACGCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0AC47 UniProtKB Entry Name FRDB_ECOLI GenBank Protein ID 7019629 GenBank Gene ID J01611 - General References
- Cole ST, Grundstrom T, Jaurin B, Robinson JJ, Weiner JH: Location and nucleotide sequence of frdB, the gene coding for the iron-sulphur protein subunit of the fumarate reductase of Escherichia coli. Eur J Biochem. 1982 Aug;126(1):211-6. [Article]
- Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Grundstrom T, Jaurin B: Overlap between ampC and frd operons on the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1111-5. [Article]
- Werth MT, Cecchini G, Manodori A, Ackrell BA, Schroder I, Gunsalus RP, Johnson MK: Site-directed mutagenesis of conserved cysteine residues in Escherichia coli fumarate reductase: modification of the spectroscopic and electrochemical properties of the [2Fe-2S] cluster. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8965-9. [Article]
- VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
- Iverson TM, Luna-Chavez C, Cecchini G, Rees DC: Structure of the Escherichia coli fumarate reductase respiratory complex. Science. 1999 Jun 18;284(5422):1961-6. [Article]
- Iverson TM, Luna-Chavez C, Croal LR, Cecchini G, Rees DC: Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. J Biol Chem. 2002 May 3;277(18):16124-30. Epub 2002 Feb 15. [Article]