Large structural protein
Details
- Name
- Large structural protein
- Synonyms
- Protein L
- Replicase
- Transcriptase
- Gene Name
- L
- Organism
- Rabies virus (strain Pasteur vaccins / PV)
- Amino acid sequence
>lcl|BSEQ0052121|Large structural protein MLDPGEVYDDPIDPIELEAEPRGTPTVPNILRNSDYNLNSPLIEDPARLMLEWLKTGNRP YRMTLTDNCSRSFRVLKDYFKKVDLGSLKVGGMAAQSMISLWLYGAHSESNRSRRCITDL AHFYSKSSPIEKLLNLTLGNRGLRIPPEGVLSCLERVDYDNAFGRYLANTYSSYLFFHVI TLYMNALDWDEEKTILALWKDLTSVDIGKDLVKFKDQIWGLLIVTKDFVYSQSSNCLFDR NYTLMLKDLFLSRFNSLMVLLSPPEPRYSDDLISQLCQLYIAGDQVLSMCGNSGYEVIKI LEPYVVNSLVQRAEKFRPLIHSLGDFPVFIKDKVSQLEETFGSCARRFFRALDQFDNIHD LVFVYGCYRHWGHPYIDYRKGLSKLYDQVHIKKVIDKSYQECLASDLARRILRWGFDKYS KWYLDSRFLARDHPLTPYIKTQTWPPKHIVDLVGDTWHKLPITQIFEIPESMDPSEILDD KSHSFTRTRLASWLSENRGGPVPSEKVIITALSKPPVNPREFLKSIDLGGLPDEDLIIGL KPKERELKIEGRFFALMSWNLRLYFVITEKLLANYILPLFDALTMTDNLNKVFKKLIDRV TGQGLLDYSRVTYAFHLDYEKWNNHQRLESTEDVFSVLDQVFGLKRVFSRTHEFFQKSWI YYSDRSDLIGLREDQIYCLDASNGPTCWNGQDGGLEGLRQKGWSLVSLLMIDRESQIRNT RTKVLAQGDNQVLCPTYMLSPGLSQEGLLYELESISRNAFSIYRAVEEGASKLGLIIKKE ETMCSYDFLIYGKTPLFRGNILVPESKRWARVSCVSNDQIVNLANIMSTVSTNALTVAQH SQSLIKPMRDFLLMSVQAVFHYLLFSPILKGRVYKILSAEGESFLLAMSRIIYLDPSLGG VSGMSLGRFHIRQFSDPVSEGLSFWREIWLSSHESWIHALCQEAGNPDLGERTLESFTRL LEDPTTLNIRGGASPTILLKDAIRKALYDEVDKVENSEFREAILLSKTHRDNFILFLTSV EPLFPRFLSELFSSSFLGIPESIIGLIQNSRTIRRQFRKSLSKTLEESFYNSEIHGISRM TQTPQRVGGVWPCSSERADLLREISWGRKVVGTTVPHPSEMLGLLPKSSISCTCGATGGG NPRVSVSVLPSFDQSFFCTGPLKGYLGSSTSMSTQLFHAWEKVTNVHVVKRALSLKESIN WFITRDSNLAQTLIRNIVSLTGPDFPLEEAPVFKRTGSALHRFKSARYSEGGYSSVCPNL LSHISVSTDTMSDLTQDGKNYDFMFQPLMLYAQTWTSELVQRDTRLRDSTFHWHLQCNRC VRPIDDVTLETSQIFEFPDVSKRISRMVSGAVPHFQRLPDIRLRPGDFESLSGREKSHHI GSAQGLLYSILVAIHDSGYNDGTIFPVNIYGKVSPRDYLRGLARGVLIGSSICFLTRMTN ININRPLELISGVISYILLRLDNHPSLYIMLREPSFREEIFSIPQKIPAAYPTTMKEGNR SILCYLQHVLRYEREVITASPENDWLWIFSDFRSAKMTYLTLITYQSHLLLQRVERNLSK SMRDNLRQLSSLMRQVLGGHGEDTLESDDNIQRLLKDSLRRTRWVDQEVRHAARTMTGDY SPNKKVSRKVGCSEWVCSAQQVAVSTSANPAPVSELDIRALSKRFQNPLISGLRVVQWAT GAHYKLKPILDDLNVFPSLCLVVGDGSGGISRAVLNMFPDAKLVFNSLLEVNDLMASGTH PLPPSAIMRGGNDIVSRVIDFDSIWEKPSDLRNLATWKYFQSVQKQVNMSYDLIICDAEV TDIASINRITLLMSDFALSIDGPLYLVFKTYGTMLVNPNYKAIQHLSRAFPSVTGFITQV TSSFSSELYLRFSKRGKFFRDAEYLTSSTLREMSLVLFNCSSPKSEMQRARSLNYQDLVR GFPEEIISNPYNEMIITLIDSDVESFLVHKMVDDLELQRGTLSKVAIIIAIMIVFSNRVF NVSKPLTDPLFYPPSDPKILRHFNICCSTMMYLSTALGDVPSFARLHDLYNRPITYYFRK QVILGNVYLSWSWSNDTSVFKRVACNSSLSLSSHWIRLIYKIVKTTRLVGSIKDLSGEVE RHLHRYNRWITLENIRSRSSLLDYSCLCIGYSWKPAHAKTLV
- Number of residues
- 2142
- Molecular Weight
- 244484.195
- Theoretical pI
- Not Available
- GO Classification
- FunctionsATP binding / mRNA (guanine-N7-)-methyltransferase activity / RNA-directed 5'-3' RNA polymerase activityProcessesnegative stranded viral RNA replicationComponentshost cell cytoplasm / virion
- General Function
- RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
- Specific Function
- Atp binding
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Virion
- Gene sequence
>lcl|BSEQ0052122|Large structural protein (L) ATGCTCGATCCTGGAGAGGTCTATGATGACCCTATTGACCCAATCGAGTTAGAGGCTGAA CCCAGAGGAACCCCCACTGTCCCCAACATCTTGAGGAACTCTGACTACAATCTCAACTCT CCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAATGGTTAAAAACAGGGAATAGACCT TATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTTCAGAGTTTTGAAAGATTATTTC AAGAAGGTAGATTTGGGTTCCCTCAAGGTGGGCGGAATGGCTGCACAGTCAATGATTTCT CTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGGAGATGTATAACAGACTTG GCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTAAATCTCACGCTAGGAAAT AGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGAGGGTTGATTATGAT AATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTCTTCCATGTAATC ACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGCATTATGGAAA GATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAATATGGGGA CTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTTGACAGA AACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGTCTTA CTTTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAGCTATGCCAGCTGTAC ATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATA TTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATT CATTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTCGAAGAGACG TTCGGTTCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGAC TTGGTTTTTGTGTATGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAG GGTCTGTCAAAACTATATGATCAGGTTCACATTAAAAAAGTGATAGATAAGTCCTACCAG GAGTGCTTAGCAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCC AAGTGGTATCTGGATTCACGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAA ACCCAAACATGGCCACCCAAACATATTGTAGATTTGGTGGGGGATACATGGCACAAGCTC CCGATCACGCAAATCTTTGAGATTCCTGAATCAATGGATCCATCAGAAATATTGGATGAC AAATCACATTCTTTCACCAGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGG CCTGTTCCTAGCGAAAAAGTTATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGA GAGTTTCTGAAGTCTATAGACCTCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTC AAGCCAAAGGAACGGGAATTGAAGATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAAT CTAAGATTGTATTTTGTCATCACTGAAAAACTCTTGGCCAACTACATCTTGCCACTTTTT GACGCGCTGACTATGACAGACAACCTGAACAAGGTGTTTAAAAAGCTGATCGACAGGGTC ACCGGGCAAGGGCTTCTGGACTATTCAAGGGTCACATATGCATTTCACCTGGACTATGAA AAGTGGAACAACCATCAAAGATTAGAGTCAACAGAGGATGTATTTTCTGTCCTAGATCAA GTGTTTGGATTGAAGAGAGTGTTTTCTAGAACACACGAGTTTTTTCAGAAGTCCTGGATC TATTATTCAGACAGATCAGACCTCATCGGGTTACGGGAGGATCAAATATACTGCTTAGAT GCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGGCGGGCTAGAAGGCTTACGGCAG AAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAGAATCTCAAATCAGGAACACA AGAACCAAAGTACTAGCTCAAGGAGACAACCAGGTTTTATGTCCGACATATATGTTGTCG CCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGCATATCAAGGAATGCATTT TCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAACTAGGGCTGATCATCAAGAAAGAA GAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTGTTTAGAGGTAAC ATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAATGACCAAATA GTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAACGCGCTAACAGTGGCACAACAC TCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCAGTCTTT CACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGCTGAA GGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAGGG GTATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAA GGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCACGAGTCCTGGATTCACGCGTTG TGTCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTT CTAGAAGATCCTACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAG GATGCAATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAGAACTCAGAGTTTCGA GAGGCAATCCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAACATCTGTT GAGCCTCTGTTTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCC GAGTCAATCATTGGACTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGT CTCTCAAAAACTTTAGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATG ACCCAGACACCTCAGAGGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTA CTTAGGGAGATCTCTTGGGGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAG ATGTTGGGGTTACTTCCCAAGTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGC AATCCTAGAGTTTCTGTATCAGTACTCCCGTCTTTTGATCAGTCATTTTTTTGCACGGGG CCCCTAAAGGGGTACTTGGGCTCGTCCACCTCTATGTCGACCCAGCTATTCCATGCATGG GAAAAAGTCACTAATGTTCATGTGGTGAAGAGAGCTCTATCGTTAAAAGAATCTATAAAC TGGTTCATTACTAGAGATTCCAACTTGGCTCAAACTCTAATTAGGAACATTGTGTCTCTG ACAGGCCCTGATTTCCCTCTAGAGGAGGCCCCTGTTTTCAAAAGGACGGGGTCAGCCTTG CATAGGTTCAAGTCTGCCAGATACAGCGAAGGAGGGTATTCTTCTGTATGCCCGAACCTC CTCTCTCATATTTCTGTTAGTACAGACACCATGTCTGATTTGACCCAAGACGGGAAGAAC TACGATTTCATGTTCCAGCCATTGATGCTTTATGCACAGACATGGACATCAGAGCTGGTA CAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTGGCACCTCCAATGCAACAGGTGT GTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGATCTTCGAGTTTCCGGATGTG TCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCACTTCCAGAGGCTTCCCGAT ATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGAAAAGTCTCACCATATC GGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACGACTCAGGATACAAT GATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGAGACTATTTGAGA GGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACGAGAATGACAAAT ATCAATATTAATAGACCTCTTGAATTGATCTCAGGGGTAATCTCATATATTCTCCTGAGG CTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTTTTAGAGAAGAGATA TTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAACAGA TCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGGTAATCACGGCGTCT CCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTA ACCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAG AGTATGAGAGATAACCTGCGACAATTGAGTTCCTTGATGAGGCAGGTGCTGGGCGGGCAC GGAGAAGATACCTTAGAGTCAGACGACAACATTCAACGACTACTAAAAGACTCTTTACGA AGGACAAGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTAC AGCCCCAACAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAA CAGGTTGCAGTCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCC CTCTCTAAGAGGTTCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACC GGTGCTCATTATAAGCTTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGC CTTGTAGTTGGGGACGGGTCAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGAT GCCAAGCTTGTGTTCAACAGTCTTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACAT CCACTGCCTCCTTCAGCAATCATGAGGGGAGGAAATGATATCGTCTCCAGAGTGATAGAT TTTGACTCAATCTGGGAAAAACCGTCCGACTTGAGAAACTTGGCTACCTGGAAATACTTC CAGTCAGTCCAAAAGCAGGTCAACATGTCCTATGACCTCATTATTTGCGATGCAGAAGTT ACTGACATTGCATCTATCAACCGGATAACCCTGTTAATGTCCGATTTTGCATTGTCTATA GATGGACCACTCTATTTGGTCTTCAAAACTTATGGGACTATGCTAGTAAATCCAAACTAC AAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCACAGGGTTTATCACCCAAGTA ACTTCGTCTTTTTCATCTGAGCTCTACCTTCGATTCTCCAAACGAGGGAAGTTTTTCAGA GATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTGTGTTATTCAATTGT AGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGGATCTTGTGAGA GGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCTGATTGAC AGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTGGATGATCTTGAGTTACAGAGGGGA ACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTC AACGTTTCCAAACCCCTAACTGACCCCTTGTTCTATCCACCGTCTGATCCCAAAATCCTG AGGCACTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTC CCTAGCTTCGCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAG CAAGTCATTCTAGGGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTC AAAAGGGTAGCCTGTAATTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTAC AAGATAGTGAAGACTACCAGACTCGTTGGCAGCATCAAGGATCTATCCGGAGAAGTGGAA AGACACCTTCATAGGTACAACAGGTGGATCACCCTAGAGAATATCAGATCTAGATCATCC CTACTAGACTACAGTTGCCTGTGCATCGGATACTCCTGGAAGCCTGCCCATGCTAAGACT CTTGTGTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11213 UniProtKB Entry Name L_RABVP - General References
- Tordo N, Poch O, Ermine A, Keith G, Rougeon F: Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology. 1988 Aug;165(2):565-76. doi: 10.1016/0042-6822(88)90600-9. [Article]
- Tordo N, Poch O, Ermine A, Keith G, Rougeon F: Walking along the rabies genome: is the large G-L intergenic region a remnant gene? Proc Natl Acad Sci U S A. 1986 Jun;83(11):3914-8. doi: 10.1073/pnas.83.11.3914. [Article]