GDP-mannose 6-dehydrogenase
Details
- Name
- GDP-mannose 6-dehydrogenase
- Synonyms
- 1.1.1.132
- GMD
- Gene Name
- algD
- Organism
- Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
- Amino acid sequence
>lcl|BSEQ0019191|GDP-mannose 6-dehydrogenase MRISIFGLGYVGAVCAGCLSARGHEVIGVDVSSTKIDLINQGKSPIVEPGLEALLQQGRQ TGRLSGTTDFKKAVLDSDVSFICVGTPSKKNGDLDLGYIETVCREIGFAIREKSERHTVV VRSTVLPGTVNNVVIPLIEDCSGKKAGVDFGVGTNPEFLRESTAIKDYDFPPMTVIGELD KQTGDLLEEIYRELDAPIIRKTVEVAEMIKYTCNVWHAAKVTFANEIGNIAKAVGVDGRE VMDVICQDHKLNLSRYYMRPGFAFGGSCLPKDVRALTYRASQLDVEHPMLGSLMRSNSNQ VQKAFDLITSHDTRKVGLLGLSFKAGTDDLRESPLVELAEMLIGKGYELRIFDRNVEYAR VHGANKEYIESKIPHVSSLLVSDLDEVVASSDVLVLGNGDELFVDLVNKTPSGKKLVDLV GFMPHTTTAQAEGICW
- Number of residues
- 436
- Molecular Weight
- 47599.155
- Theoretical pI
- 5.35
- GO Classification
- FunctionsGDP-mannose 6-dehydrogenase activity / NAD binding / UDP-glucose 6-dehydrogenase activityProcessesalginic acid biosynthetic process / UDP-glucuronate biosynthetic process
- General Function
- Udp-glucose 6-dehydrogenase activity
- Specific Function
- Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0019192|GDP-mannose 6-dehydrogenase (algD) ATGCGAATCAGCATCTTTGGTTTGGGCTATGTCGGTGCAGTATGTGCTGGCTGCCTGTCG GCACGCGGTCATGAAGTCATTGGTGTGGATGTCTCCAGCACCAAGATCGACCTGATCAAC CAGGGCAAGTCGCCCATCGTCGAACCGGGCCTGGAAGCGTTGTTGCAGCAAGGCCGGCAG ACCGGACGGCTGTCGGGCACCACCGACTTCAAGAAGGCCGTGCTGGACTCCGACGTATCG TTCATCTGCGTCGGCACGCCGAGCAAGAAGAACGGCGACCTGGACCTGGGCTACATCGAG ACCGTCTGCCGCGAGATCGGCTTCGCCATCCGCGAGAAGTCCGAACGCCACACCGTGGTG GTGCGCAGCACCGTACTGCCGGGCACCGTCAACAACGTGGTGATCCCGCTGATCGAGGAC TGCTCGGGCAAGAAGGCCGGGGTCGACTTCGGCGTCGGCACCAACCCCGAATTCCTCCGC GAGAGCACCGCGATCAAGGACTACGACTTCCCGCCGATGACCGTGATCGGCGAACTGGAC AAGCAGACCGGCGACCTTCTCGAGGAAATCTACCGCGAGCTGGACGCGCCGATCATCCGC AAGACCGTCGAGGTCGCCGAGATGATCAAGTACACCTGCAACGTCTGGCACGCCGCCAAG GTCACCTTCGCCAACGAGATCGGCAACATCGCCAAGGCGGTCGGCGTCGACGGCCGCGAG GTGATGGACGTGATCTGCCAGGACCACAAGCTCAACCTGTCGCGCTACTACATGCGTCCC GGCTTCGCCTTCGGCGGCTCCTGCCTGCCCAAGGATGTACGCGCCCTCACCTATCGCGCC AGCCAGCTGGACGTCGAGCACCCGATGCTCGGTTCGTTGATGCGCAGCAACTCCAACCAG GTGCAGAAGGCCTTCGATCTCATCACCAGCCACGACACCCGCAAGGTCGGCCTGCTCGGC CTGTCGTTCAAGGCCGGCACCGACGATTTGCGCGAAAGCCCGCTGGTGGAGCTGGCCGAG ATGCTCATCGGCAAGGGCTACGAGTTGCGCATCTTCGACCGCAACGTCGAATACGCGCGT GTCCACGGGGCCAACAAGGAATACATCGAGTCGAAGATCCCGCACGTCTCCTCACTGCTG GTCTCCGACCTCGACGAAGTGGTGGCGAGTTCCGATGTGCTGGTGCTGGGCAATGGCGAC GAGCTGTTCGTCGACCTGGTGAACAAGACCCCGAGCGGCAAGAAGCTGGTCGACCTGGTG GGCTTCATGCCGCACACCACCACTGCCCAGGCCGAGGGCATCTGCTGGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11759 UniProtKB Entry Name ALGD_PSEAE GenBank Protein ID 45268 GenBank Gene ID Y00337 - General References
- Deretic V, Gill JF, Chakrabarty AM: Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene. Nucleic Acids Res. 1987 Jun 11;15(11):4567-81. [Article]
- Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
- Roychoudhury S, May TB, Gill JF, Singh SK, Feingold DS, Chakrabarty AM: Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa. J Biol Chem. 1989 Jun 5;264(16):9380-5. [Article]
- Roychoudhury S, Chakrabarty K, Ho YK, Chakrabarty AM: Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolysis. J Biol Chem. 1992 Jan 15;267(2):990-6. [Article]
- Maharaj R, May TB, Wang SK, Chakrabarty AM: Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa. Gene. 1993 Dec 22;136(1-2):267-9. [Article]
- Naught LE, Gilbert S, Imhoff R, Snook C, Beamer L, Tipton P: Allosterism and cooperativity in Pseudomonas aeruginosa GDP-mannose dehydrogenase. Biochemistry. 2002 Jul 30;41(30):9637-45. [Article]
- Snook CF, Tipton PA, Beamer LJ: Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa. Biochemistry. 2003 Apr 29;42(16):4658-68. [Article]