6,7-dimethyl-8-ribityllumazine synthase

Details

Name
6,7-dimethyl-8-ribityllumazine synthase
Synonyms
  • 2.5.1.78
  • DMRL synthase
  • Heavy riboflavin synthase beta subunit
  • HRS beta subunit
Gene Name
ribH
Organism
Bacillus subtilis (strain 168)
Amino acid sequence
>lcl|BSEQ0019174|6,7-dimethyl-8-ribityllumazine synthase
MNIIQGNLVGTGLKIGIVVGRFNDFITSKLLSGAEDALLRHGVDTNDIDVAWVPGAFEIP
FAAKKMAETKKYDAIITLGTVIRGATTHYDYVCNEAAKGIAQAANTTGVPVIFGIVTTEN
IEQAIERAGTKAGNKGVDCAVSAIEMANLNRSFE
Number of residues
154
Molecular Weight
16286.485
Theoretical pI
5.19
GO Classification
Functions
6,7-dimethyl-8-ribityllumazine synthase activity / transferase activity
Processes
riboflavin biosynthetic process
Components
riboflavin synthase complex
General Function
Transferase activity
Specific Function
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. Is able to use the non-natural R enantiomer of 3,4-dihydroxy-2-butanone 4-phosphate as a substrate, but with less efficiency than the natural S enantiomer. Cannot use unphosphorylated 3,4-dihydroxy-2-butanone, 3,4-dihydroxy-2-butanone 3-phosphate or diacetyl as substrates.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0019175|6,7-dimethyl-8-ribityllumazine synthase (ribH)
ATGAATATCATACAAGGAAATTTAGTTGGTACAGGTCTTAAAATCGGAATCGTAGTAGGA
AGATTTAATGATTTTATTACGAGCAAGCTGCTGAGCGGAGCAGAAGATGCGCTGCTCAGA
CATGGCGTAGACACAAATGACATTGATGTGGCTTGGGTTCCAGGCGCATTTGAAATACCG
TTTGCTGCGAAAAAAATGGCGGAAACAAAAAAATATGATGCTATTATCACATTGGGCACT
GTCATCAGAGGCGCAACGACACATTACGATTATGTCTGCAATGAAGCTGCAAAAGGCATC
GCGCAAGCAGCAAACACTACTGGTGTACCTGTCATCTTTGGAATTGTAACAACTGAAAAC
ATCGAACAGGCTATCGAGCGTGCCGGCACAAAAGCGGGCAACAAAGGTGTAGATTGTGCT
GTTTCTGCCATTGAAATGGCAAATTTAAACCGCTCATTTGAATAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP11998
UniProtKB Entry NameRISB_BACSU
GenBank Protein ID410128
GenBank Gene IDL09228
General References
  1. Ludwig HC, Lottspeich F, Henschen A, Ladenstein R, Bacher A: Heavy riboflavin synthase of Bacillus subtilis. Primary structure of the beta subunit. J Biol Chem. 1987 Jan 25;262(3):1016-21. [Article]
  2. Sorokin A, Zumstein E, Azevedo V, Ehrlich SD, Serror P: The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data. Mol Microbiol. 1993 Oct;10(2):385-95. [Article]
  3. Fischer M, Haase I, Kis K, Meining W, Ladenstein R, Cushman M, Schramek N, Huber R, Bacher A: Enzyme catalysis via control of activation entropy: site-directed mutagenesis of 6,7-dimethyl-8-ribityllumazine synthase. J Mol Biol. 2003 Feb 21;326(3):783-93. [Article]
  4. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
  5. Graumann P, Schroder K, Schmid R, Marahiel MA: Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol. 1996 Aug;178(15):4611-9. [Article]
  6. Bacher A, Ludwig HC, Schnepple H, Ben-Shaul Y: Heavy riboflavin synthase from Bacillus subtilis. Quaternary structure and reaggregation. J Mol Biol. 1986 Jan 5;187(1):75-86. [Article]
  7. Kis K, Volk R, Bacher A: Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase. Biochemistry. 1995 Mar 7;34(9):2883-92. [Article]
  8. Ladenstein R, Schneider M, Huber R, Bartunik HD, Wilson K, Schott K, Bacher A: Heavy riboflavin synthase from Bacillus subtilis. Crystal structure analysis of the icosahedral beta 60 capsid at 3.3 A resolution. J Mol Biol. 1988 Oct 20;203(4):1045-70. [Article]
  9. Ritsert K, Huber R, Turk D, Ladenstein R, Schmidt-Base K, Bacher A: Studies on the lumazine synthase/riboflavin synthase complex of Bacillus subtilis: crystal structure analysis of reconstituted, icosahedral beta-subunit capsids with bound substrate analogue inhibitor at 2.4 A resolution. J Mol Biol. 1995 Oct 13;253(1):151-67. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB041625-Nitro-6-ribityl-amino-2,4(1H,3H)-pyrimidinedioneexperimentalunknownDetails