Dihydrolipoyl dehydrogenase
Details
- Name
- Dihydrolipoyl dehydrogenase
- Synonyms
- 1.8.1.4
- Dihydrolipoamide dehydrogenase
- E3 component of 2-oxoglutarate dehydrogenase complex
- Gene Name
- lpd
- Organism
- Pseudomonas fluorescens
- Amino acid sequence
>lcl|BSEQ0016951|Dihydrolipoyl dehydrogenase MSQKFDVVVIGAGPGGYVAAIRAAQLGLKTACIEKYIGKEGKVALGGTCLNVGCIPSKAL LDSSYKYHEAKEAFKVHGIEAKGVTIDVPAMVARKANIVKNLTGGIATLFKANGVTSFEG HGKLLANKQVEVTGLDGKTQVLEAENVIIASGSRPVEIPPAPLSDDIIVDSTGALEFQAV PKKLGVIGAGVIGLELGSVWARLGAEVTVLEALDKFLPAADEQIAKEALKVLTKQGLNIR LGARVTASEVKKKQVTVTFTDANGEQKETFDKLIVAVGRRPVTTDLLAADSGVTLDERGF IYVDDHCKTSVPGVFAIGDVVRGAMLAHKASEEGVMVAERIAGHKAQMNYDLIPSVIYTH PEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTTGLVKVIADAKTDRVLGVHVI GPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGHAIHIANRKKR
- Number of residues
- 478
- Molecular Weight
- 50150.535
- Theoretical pI
- 6.96
- GO Classification
- Functionsdihydrolipoyl dehydrogenase activity / flavin adenine dinucleotide bindingProcessescell redox homeostasis / glycolytic processComponentscytoplasm
- General Function
- Flavin adenine dinucleotide binding
- Specific Function
- The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0005835|1437 bp ATGAGCCAGAAATTCGACGTGGTAGTGATTGGTGCCGGCCCCGGCGGTTACGTTGCCGCC ATCCGCGCTGCCCAGCTGGGCCTGAAGACCGCCTGCATCGAGAAGTACATCGGCAAGGAA GGCAAGGTGGCGCTCGGCGGTACCTGCCTGAACGTTGGCTGCATCCCGTCCAAGGCGCTG CTGGACAGCTCCTACAAGTACCATGAGGCCAAAGAAGCCTTCAAAGTCCATGGTATCGAA GCCAAGGGCGTCACCATCGACGTTCCGGCGATGGTTGCGCGCAAGGCCAACATCGTCAAG AACCTGACCGGCGGCATCGCTACCCTGTTCAAGGCCAACGGCGTGACCTCCTTCGAAGGC CACGGCAAGCTGCTGGCCAACAAGCAGGTGGAAGTGACCGGTCTGGACGGCAAGACCCAG GTCCTGGAAGCCGAGAACGTGATCATCGCTTCCGGCTCCCGCCCGGTGGAGATCCCGCCG GCCCCGCTGAGCGACGACATCATCGTCGACTCCACCGGCGCCCTGGAATTCCAGGCCGTG CCGAAGAAGCTGGGTGTGATCGGCGCCGGCGTCATCGGCCTGGAGCTGGGTTCGGTATGG GCCCGCCTGGGTGCCGAGGTGACCGTGCTGGAAGCCCTGGACAAGTTCCTCCCGGCTGCC GACGAGCAGATCGCCAAGGAAGCCCTGAAGGTCCTGACCAAGCAAGGCCTGAACATTCGC CTGGGCGCCCGCGTGACCGCCTCGGAAGTGAAGAAGAAGCAGGTCACCGTGACCTTCACC GATGCCAACGGCGAGCAGAAGGAAACCTTCGACAAGCTGATCGTCGCGGTCGGCCGTCGC CCGGTGACCACCGACCTGCTGGCCGCCGACAGCGGCGTGACCCTGGACGAGCGTGGCTTC ATCTACGTCGACGACCACTGCAAGACCAGCGTTCCGGGCGTCTTCGCCATCGGTGACGTG GTTCGCGGCGCGATGCTCGCGCACAAGGCCTCGGAAGAGGGCGTGATGGTCGCCGAGCGC ATCGCCGGCCACAAGGCGCAGATGAACTACGACCTGATCCCGTCGGTCATCTACACCCAC CCGGAAATCGCCTGGGTCGGCAAGACCGAGCAGACCCTCAAGGCCGAGGGCGTCGAAGTC AACGTCGGCACCTTCCCGTTCGCCGCCAGCGGTCGTGCGATGGCTGCCAACGACACCACC GGCCTGGTCAAGGTCATCGCCGATGCGAAGACCGACCGCGTCCTGGGCGTCCACGTGATC GGCCCGAGCGCCGCCGAGCTGGTACAGCAAGGCGCGATCGGCATGGAATTCGGCACCAGC GCCGAAGATCTGGGCATGATGGTCTTCTCCCACCCGACGCTGTCCGAAGCCCTGCACGAA GCTGCCCTGGCGGTGAATGGCCATGCCATCCACATCGCCAACCGCAAGAAGCGCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P14218 UniProtKB Entry Name DLDH_PSEFL GenBank Gene ID M28356 - General References
- Benen JA, Van Berkel WJ, Van Dongen WM, Muller F, De Kok A: Molecular cloning and sequence determination of the lpd gene encoding lipoamide dehydrogenase from Pseudomonas fluorescens. J Gen Microbiol. 1989 Jul;135(7):1787-97. [Article]
- Mattevi A, Obmolova G, Kalk KH, van Berkel WJ, Hol WG: Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties. J Mol Biol. 1993 Apr 20;230(4):1200-15. [Article]