Elastase

Details

Name
Elastase
Synonyms
  • 3.4.24.26
  • Neutral metalloproteinase
  • PAE
  • Pseudolysin
Gene Name
lasB
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Amino acid sequence
>lcl|BSEQ0020535|Elastase
MKKVSTLDLLFVAIMGVSPAAFAADLIDVSKLPSKAAQGAPGPVTLQAAVGAGGADELKA
IRSTTLPNGKQVTRYEQFHNGVRVVGEAITEVKGPGKSVAAQRSGHFVANIAADLPGSTT
AAVSAEQVLAQAKSLKAQGRKTENDKVELVIRLGENNIAQLVYNVSYLIPGEGLSRPHFV
IDAKTGEVLDQWEGLAHAEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMN
SSTDDSKTTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFKLYRDWFGTSPLTHKLYMK
VHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQNSGLIYRGQSGGMN
EAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYMDQPSRDGRSIDNASQYYNGIDVH
HSSGVYNRAFYLLANSPGWDTRKAFEVFVDANRYYWTATSNYNSGACGVIRSAQNRNYSA
ADVTRAFSTVGVTCPSAL
Number of residues
498
Molecular Weight
53686.705
Theoretical pI
6.74
GO Classification
Functions
metal ion binding / metalloendopeptidase activity
Processes
pathogenesis
Components
extracellular region
General Function
Metalloendopeptidase activity
Specific Function
Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase (PubMed:11533066). Autocatalyses processing of its pro-peptide (PubMed:9642203, PubMed:1744034). Processes the pro-peptide of pro-chitin-binding protein (cbpD) (PubMed:9642203). Involved in the pathogenesis of P.aeruginosa infections.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Secreted
Gene sequence
>lcl|BSEQ0020536|Elastase (lasB)
ATGAAGAAGGTTTCTACGCTTGACCTGTTGTTCGTTGCGATCATGGGTGTTTCGCCGGCC
GCTTTTGCCGCCGACCTGATCGACGTGTCCAAACTCCCCAGCAAGGCTGCCCAGGGCGCG
CCCGGCCCGGTCACCTTGCAAGCCGCGGTCGGCGCTGGCGGTGCCGACGAACTGAAAGCG
ATCCGCAGCACGACCCTGCCCAACGGCAAGCAGGTCACCCGCTACGAGCAATTCCACAAC
GGCGTACGGGTGGTCGGCGAAGCCATCACCGAAGTCAAGGGTCCCGGCAAGAGCGTGGCG
GCGCAGCGCAGCGGCCATTTCGTCGCCAACATCGCTGCCGACCTGCCGGGCAGCACCACC
GCGGCGGTATCCGCCGAGCAGGTGCTGGCCCAGGCCAAGAGCCTGAAGGCCCAGGGCCGC
AAGACCGAGAATGACAAAGTGGAACTGGTGATCCGCCTGGGCGAGAACAACATCGCCCAA
CTGGTCTACAACGTCTCCTACCTGATTCCCGGCGAGGGACTGTCGCGGCCGCATTTCGTC
ATCGACGCCAAGACCGGCGAAGTGCTCGATCAGTGGGAAGGCCTGGCCCACGCCGAGGCG
GGCGGCCCCGGCGGCAACCAGAAGATCGGCAAGTACACCTACGGTAGCGACTACGGTCCG
CTGATCGTCAACGACCGCTGCGAGATGGACGACGGCAACGTCATCACCGTCGACATGAAC
AGCAGCACCGACGACAGCAAGACCACGCCGTTCCGCTTCGCCTGCCCGACCAACACCTAC
AAGCAGGTCAACGGCGCCTATTCGCCGCTGAACGACGCGCATTTCTTCGGCGGCGTGGTG
TTCAAACTGTACCGGGACTGGTTCGGCACCAGCCCGCTGACCCACAAGCTGTACATGAAG
GTGCACTACGGGCGCAGCGTGGAGAACGCCTACTGGGACGGCACGGCGATGCTCTTCGGC
GACGGCGCCACCATGTTCTATCCGCTGGTGTCGCTGGACGTGGCGGCCCACGAGGTCAGC
CACGGCTTCACCGAGCAGAACTCCGGGCTGATCTACCGCGGGCAATCAGGCGGAATGAAC
GAAGCGTTCTCCGACATGGCCGGCGAGGCTGCCGAGTTCTATATGCGCGGCAAGAACGAC
TTCCTGATCGGCTACGACATCAAGAAGGGCAGCGGTGCGCTGCGCTACATGGACCAGCCC
AGCCGCGACGGGCGATCCATCGACAACGCGTCGCAGTACTACAACGGCATCGACGTGCAC
CACTCCAGCGGCGTGTACAACCGTGCGTTCTACCTGTTGGCCAATTCGCCGGGCTGGGAT
ACCCGCAAGGCCTTCGAGGTGTTCGTCGACGCCAACCGCTACTACTGGACCGCCACCAGC
AACTACAACAGCGGCGCCTGCGGGGTGATTCGCTCGGCGCAGAACCGCAACTACTCGGCG
GCTGACGTCACCCGGGCGTTCAGCACCGTCGGCGTGACCTGCCCGAGCGCGTTGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP14756
UniProtKB Entry NameELAS_PSEAE
GenBank Protein ID151210
GenBank Gene IDM19472
General References
  1. Bever RA, Iglewski BH: Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene. J Bacteriol. 1988 Sep;170(9):4309-14. [Article]
  2. Fukushima J, Yamamoto S, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Okuda K: Structural gene and complete amino acid sequence of Pseudomonas aeruginosa IFO 3455 elastase. J Bacteriol. 1989 Mar;171(3):1698-704. [Article]
  3. Tanaka E, Kawamoto S, Fukushima J, Hamajima K, Onishi H, Miyagi Y, Inami S, Morihara K, Okuda K: Detection of elastase production in Escherichia coli with the elastase structural gene from several non-elastase-producing strains of Pseudomonas aeruginosa. J Bacteriol. 1991 Oct;173(19):6153-8. [Article]
  4. Ogino H, Yokoo J, Watanabe F, Ishikawa H: Cloning and sequencing of a gene of organic solvent-stable protease secreted from Pseudomonas aeruginosa PST-01 and its expression in Escherichia coli. Biochem Eng J. 2000 Jul 1;5(3):191-200. [Article]
  5. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
  6. Kessler E, Safrin M, Peretz M, Burstein Y: Identification of cleavage sites involved in proteolytic processing of Pseudomonas aeruginosa preproelastase. FEBS Lett. 1992 Mar 16;299(3):291-3. [Article]
  7. Braun P, de Groot A, Bitter W, Tommassen J: Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa. J Bacteriol. 1998 Jul;180(13):3467-9. [Article]
  8. Schad PA, Bever RA, Nicas TI, Leduc F, Hanne LF, Iglewski BH: Cloning and characterization of elastase genes from Pseudomonas aeruginosa. J Bacteriol. 1987 Jun;169(6):2691-6. [Article]
  9. Hase CC, Finkelstein RA: Comparison of the Vibrio cholerae hemagglutinin/protease and the Pseudomonas aeruginosa elastase. Infect Immun. 1990 Dec;58(12):4011-5. [Article]
  10. McIver K, Kessler E, Ohman DE: Substitution of active-site His-223 in Pseudomonas aeruginosa elastase and expression of the mutated lasB alleles in Escherichia coli show evidence for autoproteolytic processing of proelastase. J Bacteriol. 1991 Dec;173(24):7781-9. [Article]
  11. Kessler E, Safrin M: Synthesis, processing, and transport of Pseudomonas aeruginosa elastase. J Bacteriol. 1988 Nov;170(11):5241-7. [Article]
  12. Olson JC, Ohman DE: Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions. J Bacteriol. 1992 Jun;174(12):4140-7. [Article]
  13. Cahan R, Axelrad I, Safrin M, Ohman DE, Kessler E: A secreted aminopeptidase of Pseudomonas aeruginosa. Identification, primary structure, and relationship to other aminopeptidases. J Biol Chem. 2001 Nov 23;276(47):43645-52. Epub 2001 Aug 31. [Article]
  14. Thayer MM, Flaherty KM, McKay DB: Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution. J Biol Chem. 1991 Feb 15;266(5):2864-71. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB02307N-(1-carboxy-3-phenylpropyl)phenylalanyl-alpha-asparagineexperimentalunknownDetails