Capsid scaffolding protein
Details
- Name
- Capsid scaffolding protein
- Synonyms
- APNG
- Capsid protein P40
- pPR
- Protease precursor
- Gene Name
- UL80
- Organism
- HHV-5
- Amino acid sequence
>lcl|BSEQ0002643|Capsid scaffolding protein MTMDEQQSQAVAPVYVGGFLARYDQSPDEAELLLPRDVVEHWLHAQGQGQPSLSVALPLN INHDDTAVVGHVAAMQSVRDGLFCLGCVTSPRFLEIVRRASEKSELVSRGPVSPLQPDKV VEFLSGSYAGLSLSSRRCDDVEAATSLSGSETTPFKHVALCSVGRRRGTLAVYGRDPEWV TQRFPDLTAADRDGLRAQWQRCGSTAVDASGDPFRSDSYGLLGNSVDALYIRERLPKLRY DKQLVGVTERESYVKASVSPEAACDIKAASAERSGDSRSQAATPAAGARVPSSSPSPPVE PPSPVQPPALPASPSVLPAESPPSLSPSEPAEAASMSHPLSAAVPAATAPPGATVAGASP AVSSLAWPHDGVYLPKDAFFSLLGASRSAVPVMYPGAVAAPPSASPAPLPLPSYPASYGA PVVGYDQLAARHFADYVDPHYPGWGRRYEPAPSLHPSYPVPPPPSPAYYRRRDSPGGMDE PPSGWERYDGGHRGQSQKQHRHGGSGGHNKRRKETAAASSSSSDEDLSFPGEAEHGRARK RLKSHVNSDGGSGGHAGSNQQQQQRYDELRDAIHELKRDLFAARQSSTLLSAALPSAASS SPTTTTVCTPTGELTSGGGETPTALLSGGAKVAERAQAGVVNASCRLATASGSEAATAGP STAGSSSCPASVVLAAAAAQAAAASQSPPKDMVDLNRRIFVAALNKLE
- Number of residues
- 708
- Molecular Weight
- 73851.155
- Theoretical pI
- 6.79
- GO Classification
- Functionsserine-type endopeptidase activityProcessesviral release from host cellComponentshost cell cytoplasm / host cell nucleus
- General Function
- Serine-type endopeptidase activity
- Specific Function
- Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein VP5 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein VP5. Cleavages products are evicted from the capsid before or during DNA packaging.Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-|-Ser-, -Ala-|-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein VP5. Multimerizes in the nucleus such as VP5 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
- Pfam Domain Function
- Peptidase_S21 (PF00716)
- Transmembrane Regions
- Not Available
- Cellular Location
- Host cytoplasm
- Gene sequence
>lcl|BSEQ0002642|2127 bp ATGACGATGGACGAGCAGCAGTCGCAGGCTGTGGCGCCGGTCTACGTGGGCGGCTTTCTC GCCCGCTACGACCAGTCTCCGGACGAGGCCGAATTGCTGTTGCCGCGGGACGTAGTGGAG CACTGGTTGCACGCGCAGGGCCAGGGACAGCCTTCGTTGTCGGTCGCGCTCCCGCTCAAC ATCAACCACGACGACACGGCCGTTGTAGGACACGTTGCGGCGATGCAGAGCGTCCGCGAC GGTCTTTTTTGCCTGGGCTGCGTCACTTCGCCCAGGTTTCTGGAGATTGTACGCCGCGCT TCGGAAAAGTCCGAGCTGGTTTCGCGCGGGCCCGTCAGTCCGCTGCAGCCAGACAAGGTG GTGGAGTTTCTCAGCGGCAGCTACGCCGGCCTCTCGCTCTCCAGCCGGCGCTGCGACGAC GTGGAGGCCGCGACGTCGCTTTCGGGCTCGGAAACCACGCCGTTCAAACACGTGGCTTTG TGCAGCGTGGGTCGGCGTCGCGGTACGTTGGCCGTGTACGGGCGCGATCCCGAGTGGGTC ACACAGCGGTTTCCAGACCTCACGGCGGCCGACCGTGACGGGCTACGTGCACAGTGGCAG CGCTGCGGCAGCACTGCTGTCGACGCGTCGGGCGATCCCTTTCGCTCAGACAGCTACGGC CTGTTGGGCAACAGCGTGGACGCGCTCTACATCCGTGAGCGACTGCCCAAGCTGCGCTAC GACAAGCAACTAGTCGGCGTGACGGAGCGCGAGTCATACGTCAAGGCGAGCGTTTCGCCT GAGGCGGCGTGCGATATTAAAGCGGCGTCCGCCGAGCGTTCGGGCGACAGCCGCAGTCAG GCCGCCACGCCGGCGGCTGGGGCGCGCGTTCCCTCTTCGTCCCCGTCGCCTCCAGTCGAA CCGCCATCTCCTGTACAGCCGCCTGCGCTTCCAGCGTCGCCGTCCGTTCTTCCCGCGGAA TCACCGCCGTCGCTTTCTCCCTCGGAGCCGGCAGAGGCGGCGTCCATGTCGCACCCTCTG AGTGCTGCGGTTCCCGCCGCTACGGCTCCTCCAGGTGCTACCGTGGCAGGTGCGTCGCCG GCTGTGTCGTCTCTAGCGTGGCCTCACGACGGAGTTTATTTACCCAAAGACGCTTTTTTC TCGCTACTTGGGGCCAGTCGCTCGGCAGTGCCCGTCATGTATCCCGGCGCCGTAGCGGCC CCTCCTTCTGCTTCGCCAGCACCGCTGCCTTTGCCGTCTTATCCCGCGTCCTACGGCGCC CCCGTCGTGGGTTACGACCAGTTGGCGGCACGTCACTTTGCGGACTACGTGGATCCCCAT TATCCCGGGTGGGGTCGGCGTTACGAGCCCGCGCCGTCTTTGCATCCGTCTTATCCCGTG CCGCCGCCACCATCACCGGCCTATTACCGTCGGCGCGACTCTCCGGGCGGTATGGATGAA CCACCGTCCGGATGGGAGCGTTACGACGGTGGTCACCGTGGTCAGTCGCAGAAGCAGCAC CGTCACGGGGGCAGCGGCGGACACAACAAACGCCGTAAGGAAACCGCGGCGGCGTCGTCG TCGTCCTCGGACGAAGACTTGAGTTTCCCAGGCGAGGCCGAGCACGGCCGGGCACGAAAG CGTCTAAAAAGTCACGTCAATAGCGACGGTGGAAGTGGCGGGCACGCGGGTTCCAATCAG CAGCAGCAACAACGTTACGATGAACTGCGGGATGCCATTCACGAGCTGAAACGCGATCTG TTTGCTGCGCGGCAGAGTTCTACGTTACTTTCGGCGGCTCTTCCCTCTGCGGCCTCTTCC TCCCCAACTACTACTACCGTGTGTACTCCCACCGGCGAGCTGACGAGTGGCGGAGGAGAA ACACCCACGGCACTTCTATCCGGAGGTGCCAAGGTAGCTGAGCGCGCTCAGGCCGGCGTG GTGAACGCCAGTTGCCGCCTCGCTACCGCGTCGGGTTCTGAGGCGGCAACGGCCGGGCCC TCGACGGCAGGTTCTTCTTCCTGCCCGGCTAGTGTCGTGTTAGCCGCCGCTGCTGCCCAA GCCGCCGCAGCTTCCCAGAGCCCGCCCAAAGACATGGTAGATCTGAATCGGCGGATTTTT GTGGCTGCGCTCAATAAGCTCGAGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P16753 UniProtKB Entry Name SCAF_HCMVA GenBank Protein ID 1780857 GenBank Gene ID X17403 - General References
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- Davison AJ, Dolan A, Akter P, Addison C, Dargan DJ, Alcendor DJ, McGeoch DJ, Hayward GS: The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J Gen Virol. 2003 Jan;84(Pt 1):17-28. [Article]
- Varnum SM, Streblow DN, Monroe ME, Smith P, Auberry KJ, Pasa-Tolic L, Wang D, Camp DG 2nd, Rodland K, Wiley S, Britt W, Shenk T, Smith RD, Nelson JA: Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome. J Virol. 2004 Oct;78(20):10960-6. [Article]
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- Gibson W: Structure and formation of the cytomegalovirus virion. Curr Top Microbiol Immunol. 2008;325:187-204. [Article]
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- Qiu X, Culp JS, DiLella AG, Hellmig B, Hoog SS, Janson CA, Smith WW, Abdel-Meguid SS: Unique fold and active site in cytomegalovirus protease. Nature. 1996 Sep 19;383(6597):275-9. [Article]
- Shieh HS, Kurumbail RG, Stevens AM, Stegeman RA, Sturman EJ, Pak JY, Wittwer AJ, Palmier MO, Wiegand RC, Holwerda BC, Stallings WC: Three-dimensional structure of human cytomegalovirus protease. Nature. 1996 Sep 19;383(6597):279-82. [Article]