Nucleoside diphosphate kinase B


Nucleoside diphosphate kinase B
  • C-myc purine-binding transcription factor PUF
  • Histidine protein kinase NDKB
  • NDK B
  • nm23-H2
  • NM23B
Gene Name
Amino acid sequence
>lcl|BSEQ0006399|Nucleoside diphosphate kinase B
Number of residues
Molecular Weight
Theoretical pI
GO Classification
ATP binding / metal ion binding / nucleoside diphosphate kinase activity / protein histidine kinase activity / RNA polymerase II regulatory region sequence-specific DNA binding / single-stranded DNA binding / transcription factor activity, sequence-specific DNA binding
cell adhesion / cellular response to drug / cellular response to fatty acid / cellular response to glucose stimulus / CTP biosynthetic process / GTP biosynthetic process / hippocampus development / integrin-mediated signaling pathway / lactation / mitophagy in response to mitochondrial depolarization / negative regulation of apoptotic process / negative regulation of gene expression / negative regulation of myeloid leukocyte differentiation / nucleobase-containing small molecule interconversion / nucleobase-containing small molecule metabolic process / nucleoside triphosphate biosynthetic process / positive regulation of epithelial cell proliferation / positive regulation of keratinocyte differentiation / positive regulation of neuron projection development / positive regulation of transcription from RNA polymerase II promoter / purine nucleotide metabolic process / pyrimidine nucleotide metabolic process / regulation of epidermis development / regulation of transcription, DNA-templated / response to amine / response to cAMP / response to testosterone / small molecule metabolic process / transcription, DNA-templated / UTP biosynthetic process
cell periphery / centrosome / cytoplasm / cytosol / extracellular exosome / intermediate filament / intracellular / lamellipodium / mitochondrial outer membrane / myelin sheath / nucleus / perinuclear region of cytoplasm / ruffle
General Function
Transcription factor activity, sequence-specific dna binding
Specific Function
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Gene sequence
>lcl|BSEQ0021684|Nucleoside diphosphate kinase B (NME2)
Chromosome Location
External Identifiers
UniProtKB IDP22392
UniProtKB Entry NameNDKB_HUMAN
GenBank Gene IDX58965
GenAtlas IDNME2
General References
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  3. Postel EH, Berberich SJ, Flint SJ, Ferrone CA: Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis. Science. 1993 Jul 23;261(5120):478-80. [Article]
  4. Valentijn LJ, Koster J, Versteeg R: Read-through transcript from NM23-H1 into the neighboring NM23-H2 gene encodes a novel protein, NM23-LV. Genomics. 2006 Apr;87(4):483-9. Epub 2006 Jan 25. [Article]
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  8. Fournier HN, Dupe-Manet S, Bouvard D, Lacombe ML, Marie C, Block MR, Albiges-Rizo C: Integrin cytoplasmic domain-associated protein 1alpha (ICAP-1alpha ) interacts directly with the metastasis suppressor nm23-H2, and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement. J Biol Chem. 2002 Jun 7;277(23):20895-902. Epub 2002 Mar 27. [Article]
  9. Iwashita S, Fujii M, Mukai H, Ono Y, Miyamoto M: Lbc proto-oncogene product binds to and could be negatively regulated by metastasis suppressor nm23-H2. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1063-8. [Article]
  10. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. [Article]
  11. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16. [Article]
  12. Wieland T, Hippe HJ, Ludwig K, Zhou XB, Korth M, Klumpp S: Reversible histidine phosphorylation in mammalian cells: a teeter-totter formed by nucleoside diphosphate kinase and protein histidine phosphatase 1. Methods Enzymol. 2010;471:379-402. doi: 10.1016/S0076-6879(10)71020-X. Epub 2010 Mar 1. [Article]
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  15. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
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  18. Morera S, Lacombe ML, Xu Y, LeBras G, Janin J: X-ray structure of human nucleoside diphosphate kinase B complexed with GDP at 2 A resolution. Structure. 1995 Dec 15;3(12):1307-14. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB00709Lamivudineapproved, investigationalunknownsubstrateDetails
DB00718Adefovir dipivoxilapproved, investigationalunknownsubstrateDetails
DB09299Tenofovir alafenamideapprovednosubstrateDetails
DB14126Tenofovirexperimental, investigationalnosubstrateDetails
DB00640Adenosineapproved, investigationalunknownsubstrateDetails
DB00300Tenofovir disoproxilapproved, investigationalunknownsubstrateDetails
DB01262Decitabineapproved, investigationalyessubstrateDetails