2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

Details

Name
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Synonyms
  • 2.7.6.3
  • 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
  • 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
  • HPPK
  • PPPK
Gene Name
folK
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016432|2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTPPLGPQDQPDYLNAAVA
LETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMK
NRGFMLWPLFEIAPELVFPDGEMLRQILHTRAFDKLNKW
Number of residues
159
Molecular Weight
18078.61
Theoretical pI
5.23
GO Classification
Functions
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity / ATP binding / kinase activity / magnesium ion binding
Processes
folic acid biosynthetic process / tetrahydrofolate biosynthetic process
General Function
Magnesium ion binding
Specific Function
Not Available
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016433|2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK)
ATGACAGTGGCGTATATTGCCATAGGCAGCAATCTGGCCTCTCCGCTGGAGCAGGTCAAT
GCTGCCCTGAAAGCATTAGGCGATATCCCTGAAAGCCACATTCTTACCGTTTCTTCGTTT
TACCGCACCCCACCGCTGGGGCCGCAAGATCAACCCGATTACTTAAACGCAGCCGTGGCG
CTGGAAACCTCTCTTGCACCTGAAGAGCTACTCAATCACACACAGCGTATTGAATTGCAG
CAAGGTCGCGTCCGCAAAGCTGAACGCTGGGGACCACGCACGCTGGATCTCGACATCATG
CTGTTTGGTAATGAAGTGATAAATACTGAACGCCTGACCGTTCCGCACTACGATATGAAG
AATCGTGGATTTATGCTGTGGCCGCTGTTTGAAATCGCGCCGGAGTTGGTGTTTCCTGAT
GGGGAGATGTTGCGTCAAATCTTACATACAAGAGCATTTGACAAATTAAACAAATGGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP26281
UniProtKB Entry NameHPPK_ECOLI
GenBank Protein ID146013
GenBank Gene IDL06495
General References
  1. Talarico TL, Ray PH, Dev IK, Merrill BM, Dallas WS: Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase. J Bacteriol. 1992 Sep;174(18):5971-7. [Article]
  2. Fujita N, Mori H, Yura T, Ishihama A: Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. Nucleic Acids Res. 1994 May 11;22(9):1637-9. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Talarico TL, Dev IK, Dallas WS, Ferone R, Ray PH: Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100. J Bacteriol. 1991 Nov;173(21):7029-32. [Article]
  6. Liu JD, Parkinson JS: Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control. J Bacteriol. 1989 Mar;171(3):1254-61. [Article]
  7. Xiao B, Shi G, Chen X, Yan H, Ji X: Crystal structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, a potential target for the development of novel antimicrobial agents. Structure. 1999 May;7(5):489-96. [Article]
  8. Stammers DK, Achari A, Somers DO, Bryant PK, Rosemond J, Scott DL, Champness JN: 2.0 A X-ray structure of the ternary complex of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase from Escherichia coli with ATP and a substrate analogue. FEBS Lett. 1999 Jul 30;456(1):49-53. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB021196-Hydroxymethyl-7,8-DihydropterinexperimentalunknownDetails
DB02596alpha,beta-Methyleneadenosine 5'-triphosphateexperimentalunknownDetails
DB031976-HydroxymethylpterinexperimentalunknownDetails
DB040476-hydroxymethylpterin diphosphateexperimentalunknownDetails
DB041586-(adenosine tetraphosphate-methyl)-7,8-dihydropterinexperimentalunknownDetails
DB046107,8-dihydro-6-hydroxymethyl-7-methyl-7-[2-phenylethyl]-pterinexperimentalunknownDetails