o-succinylbenzoate synthase
Details
- Name
- o-succinylbenzoate synthase
- Synonyms
- 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase
- 4.2.1.113
- o-succinylbenzoic acid synthase
- OSB synthase
- Gene Name
- menC
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0019324|o-succinylbenzoate synthase MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGEISPLPGFSQETWEEAQ SVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAANYRAAPLCNGDPDDLILK LADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRLDANRAWTPLKGQQFAKYVNP DYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLREPDFAFVAEEGVRAVVIKPTLTG SLEKVREQVQAAHALGLTAVISSSIESSLGLTQLARIAAWLTPDTIPGLDTLDLMQAQQV RRWPGSTLPVVEVDALERLL
- Number of residues
- 320
- Molecular Weight
- 35476.215
- Theoretical pI
- 4.57
- GO Classification
- Functionshydro-lyase activity / magnesium ion bindingProcessesmenaquinone biosynthetic process
- General Function
- Not Available
- Specific Function
- Not Available
- Pfam Domain Function
- MR_MLE_C (PF13378)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0019325|o-succinylbenzoate synthase (menC) ATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGC GACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAA GGGTGGGGGGAGATCTCCCCACTGCCGGGCTTCAGTCAGGAAACCTGGGAAGAGGCGCAA AGTGTGCTGCTTGCCTGGGTAAATAACTGGCTGGCAGGCGATTGCGAGCTACCGCAGATG CCTTCCGTGGCCTTTGGCGTAAGCTGTGCATTGGCAGAACTGACAGATACGTTGCCGCAA GCAGCCAACTACCGTGCGGCACCGCTGTGTAATGGCGATCCGGACGATCTGATCCTCAAA CTTGCAGATATGCCAGGCGAGAAAGTGGCGAAGGTCAAAGTGGGATTGTACGAAGCGGTG CGCGACGGCATGGTGGTGAATCTGTTGCTGGAGGCAATTCCGGATCTGCATTTGCGTCTT GACGCAAATCGCGCCTGGACACCGCTGAAAGGTCAGCAGTTTGCCAAATACGTTAACCCG GATTATCGCGACCGCATCGCGTTTCTCGAAGAGCCGTGCAAAACCCGCGATGATTCGCGA GCGTTTGCCCGTGAAACCGGCATTGCCATTGCCTGGGATGAAAGCCTGCGCGAGCCGGAT TTTGCCTTTGTGGCTGAAGAGGGCGTGCGCGCGGTAGTTATCAAACCCACGCTCACGGGC AGTCTGGAAAAAGTACGCGAGCAGGTACAGGCGGCGCACGCGCTGGGGCTGACGGCGGTG ATCAGTTCTTCCATTGAATCGAGCTTAGGCTTAACGCAACTGGCGCGGATTGCCGCCTGG TTAACGCCGGACACCATTCCAGGGCTGGACACGCTGGATCTGATGCAGGCGCAGCAGGTA CGTCGCTGGCCGGGTAGCACGCTGCCTGTCGTGGAAGTTGATGCACTGGAGCGGTTGTTA TGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P29208 UniProtKB Entry Name MENC_ECOLI GenBank Gene ID U00096 - General References
- Sharma V, Meganathan R, Hudspeth ME: Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli. J Bacteriol. 1993 Aug;175(15):4917-21. [Article]
- Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Palmer DR, Garrett JB, Sharma V, Meganathan R, Babbitt PC, Gerlt JA: Unexpected divergence of enzyme function and sequence: "N-acylamino acid racemase" is o-succinylbenzoate synthase. Biochemistry. 1999 Apr 6;38(14):4252-8. [Article]
- Thompson TB, Garrett JB, Taylor EA, Meganathan R, Gerlt JA, Rayment I: Evolution of enzymatic activity in the enolase superfamily: structure of o-succinylbenzoate synthase from Escherichia coli in complex with Mg2+ and o-succinylbenzoate. Biochemistry. 2000 Sep 5;39(35):10662-76. [Article]