Ferrochelatase
Details
- Name
- Ferrochelatase
- Synonyms
- 4.99.1.1
- Heme synthase
- hemF
- Protoheme ferro-lyase
- Gene Name
- hemH
- Organism
- Bacillus subtilis (strain 168)
- Amino acid sequence
>lcl|BSEQ0010912|Ferrochelatase MSRKKMGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLKDRYEAIGGISPLAQIT EQQAHNLEQHLNEIQDEITFKAYIGLKHIEPFIEDAVAEMHKDGITEAVSIVLAPHFSTF SVQSYNKRAKEEAEKLGGLTITSVESWYDEPKFVTYWVDRVKETYASMPEDERENAMLIV SAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAVGWQSEGNTPDPWLGPDVQDLTR DLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVVTDDIGASYYRPEMPNAKPEFIDAL ATVVLKKLGR
- Number of residues
- 310
- Molecular Weight
- 35347.555
- Theoretical pI
- 4.54
- GO Classification
- Functionsferrochelatase activity / metal ion bindingProcessesheme biosynthetic processComponentscytoplasm
- General Function
- Metal ion binding
- Specific Function
- Catalyzes the ferrous insertion into protoporphyrin IX.
- Pfam Domain Function
- Ferrochelatase (PF00762)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0010913|Ferrochelatase (hemH) GTGAGTAGAAAGAAAATGGGGCTTCTCGTGATGGCGTACGGCACGCCTTATAAGGAAGAA GATATTGAACGTTATTATACACATATCAGAAGAGGCAGAAAGCCTGAACCTGAAATGCTT CAAGATTTGAAAGACAGATACGAAGCGATTGGCGGCATTTCACCGCTTGCCCAAATCACA GAACAGCAGGCGCATAATCTGGAACAGCACTTGAATGAAATTCAGGATGAGATTACGTTT AAAGCGTATATCGGACTGAAGCATATCGAGCCGTTTATTGAAGACGCCGTAGCAGAGATG CATAAGGATGGCATTACAGAAGCAGTCAGCATCGTGCTGGCGCCGCATTTCTCCACGTTC AGCGTTCAGTCCTACAACAAACGCGCCAAAGAAGAGGCTGAAAAACTCGGCGGCTTAACG ATTACGTCAGTTGAAAGCTGGTACGATGAACCGAAGTTCGTGACATATTGGGTTGACCGA GTGAAGGAAACATATGCGTCAATGCCTGAGGATGAAAGAGAAAACGCAATGCTCATCGTA TCCGCACACAGCCTGCCGGAAAAAATCAAAGAATTTGGAGACCCGTACCCGGACCAGCTT CATGAATCGGCGAAATTAATTGCTGAAGGCGCAGGCGTTTCCGAATATGCTGTCGGCTGG CAAAGTGAAGGGAACACGCCTGATCCTTGGCTCGGACCCGATGTTCAGGATTTAACACGT GATTTATTCGAACAAAAAGGCTATCAAGCATTTGTATATGTTCCTGTTGGGTTTGTCGCG GATCACTTAGAAGTGCTTTATGATAATGATTATGAATGCAAAGTGGTTACTGACGATATC GGCGCAAGCTATTACCGGCCGGAAATGCCAAATGCCAAGCCTGAATTTATTGATGCTTTG GCAACAGTCGTATTAAAAAAATTAGGACGTTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P32396 UniProtKB Entry Name HEMH_BACSU GenBank Protein ID 143044 GenBank Gene ID M97208 - General References
- Hansson M, Hederstedt L: Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes. J Bacteriol. 1992 Dec;174(24):8081-93. [Article]
- Noback MA, Holsappel S, Kiewiet R, Terpstra P, Wambutt R, Wedler H, Venema G, Bron S: The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene. Microbiology. 1998 Apr;144 ( Pt 4):859-75. [Article]
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- Al-Karadaghi S, Hansson M, Nikonov S, Jonsson B, Hederstedt L: Crystal structure of ferrochelatase: the terminal enzyme in heme biosynthesis. Structure. 1997 Nov 15;5(11):1501-10. [Article]
- Lecerof D, Fodje M, Hansson A, Hansson M, Al-Karadaghi S: Structural and mechanistic basis of porphyrin metallation by ferrochelatase. J Mol Biol. 2000 Mar 17;297(1):221-32. [Article]
- Hansson M, Gough SP, Brody SS: Structure prediction and fold recognition for the ferrochelatase family of proteins. Proteins. 1997 Apr;27(4):517-22. [Article]