Porin D
Details
- Name
- Porin D
- Synonyms
- 3.4.21.-
- Imipenem/basic amino acid-specific outer membrane pore
- Outer membrane protein D2
- Gene Name
- oprD
- Organism
- Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
- Amino acid sequence
>lcl|BSEQ0017286|Porin D MKVMKWSAIALAVSAGSTQFAVADAFVSDQAEAKGFIEDSSLDLLLRNYYFNRDGKSGSG DRVDWTQGFLTTYESGFTQGTVGFGVDAFGYLGLKLDGTSDKTGTGNLPVMNDGKPRDDY SRAGGAVKVRISKTMLKWGEMQPTAPVFAAGGSRLFPQTATGFQLQSSEFEGLDLEAGHF TEGKEPTTVKSRGELYATYAGETAKSADFIGGRYAITDNLSASLYGAELEDIYRQYYLNS NYTIPLASDQSLGFDFNIYRTNDEGKAKAGDISNTTWSLAAAYTLDAHTFTLAYQKVHGD QPFDYIGFGRNGSGAGGDSIFLANSVQYSDFNGPGEKSWQARYDLNLASYGVPGLTFMVR YINGKDIDGTKMSDNNVGYKNYGYGEDGKHHETNLEAKYVVQSGPAKDLSFRIRQAWHRA NADQGEGDQNEFRLIVDYPLSIL
- Number of residues
- 443
- Molecular Weight
- 48359.97
- Theoretical pI
- 4.75
- GO Classification
- Functionsporin activity / serine-type peptidase activityProcessesbasic amino acid transport / transmembrane transportComponentsintegral component of cell outer membrane / outer membrane / pore complex
- General Function
- Serine-type peptidase activity
- Specific Function
- Porin with a specificity for basic amino acids. Also possesses serine protease activity.
- Pfam Domain Function
- OprD (PF03573)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell outer membrane
- Gene sequence
>lcl|BSEQ0017287|Porin D (oprD) ATGAAAGTGATGAAGTGGAGCGCCATTGCACTGGCGGTTTCCGCAGGTAGCACTCAGTTC GCCGTGGCCGACGCATTCGTCAGCGATCAGGCCGAAGCGAAGGGGTTCATCGAAGACAGC AGCCTCGACCTGCTGCTCCGCAACTACTATTTCAACCGTGACGGCAAGAGCGGCAGCGGG GACCGCGTCGACTGGACCCAAGGCTTCCTCACCACCTATGAATCCGGCTTCACCCAAGGC ACTGTGGGCTTCGGCGTCGATGCCTTCGGCTACCTGGGCCTGAAGCTCGACGGCACCTCC GACAAGACCGGCACCGGCAACCTGCCGGTGATGAACGACGGCAAGCCGCGCGATGACTAC AGCCGCGCCGGCGGCGCCGTGAAGGTGCGCATCTCCAAGACCATGCTGAAGTGGGGCGAG ATGCAACCGACCGCCCCGGTCTTCGCCGCTGGCGGCAGCCGCCTGTTCCCGCAGACCGCG ACCGGCTTCCAGCTGCAGAGCAGCGAATTCGAAGGGCTCGACCTCGAGGCAGGCCACTTC ACCGAGGGCAAGGAGCCGACCACCGTCAAATCGCGTGGCGAACTCTATGCCACCTACGCA GGCGAGACCGCCAAGAGCGCCGATTTCATTGGGGGCCGCTACGCAATCACCGATAACCTC AGCGCCTCCCTGTACGGCGCCGAACTCGAAGACATCTATCGCCAGTATTACCTGAACAGC AACTACACCATCCCACTGGCATCCGACCAATCGCTGGGCTTCGATTTCAACATCTACCGC ACAAACGATGAAGGCAAGGCCAAGGCCGGCGACATCAGCAACACCACTTGGTCCCTGGCG GCAGCCTACACTCTGGATGCGCACACTTTCACCTTGGCCTACCAGAAGGTCCATGGCGAT CAGCCGTTTGATTATATCGGCTTCGGCCGCAACGGCTCTGGCGCAGGTGGCGACTCGATT TTCCTCGCCAACTCTGTCCAGTACTCCGACTTCAACGGCCCTGGCGAGAAATCCTGGCAG GCTCGCTACGACCTGAACCTAGCCTCCTATGGCGTTCCCGGCCTGACTTTCATGGTCCGC TATATCAATGGCAAGGACATCGATGGCACCAAGATGTCTGACAACAACGTCGGCTATAAG AACTACGGCTACGGCGAGGATGGCAAGCACCACGAAACCAACCTCGAAGCCAAGTACGTG GTCCAGTCCGGTCCGGCCAAGGACCTGTCGTTCCGCATCCGCCAGGCCTGGCACCGTGCC AACGCCGACCAGGGCGAAGGCGACCAGAACGAGTTCCGCCTGATCGTCGACTATCCGCTG TCGATCCTGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P32722 UniProtKB Entry Name PORD_PSEAE GenBank Protein ID 45371 GenBank Gene ID X63152 - General References
- Yoneyama H, Yoshihara E, Nakae T: Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1992 Aug;36(8):1791-3. [Article]
- Huang H, Siehnel RJ, Bellido F, Rawling E, Hancock RE: Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa. FEMS Microbiol Lett. 1992 Oct 15;76(3):267-73. [Article]
- Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
- Michea-Hamzehpour M, Sanchez JC, Epp SF, Paquet N, Hughes GJ, Hochstrasser D, Pechere JC: Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins. Enzyme Protein. 1993;47(1):1-8. [Article]
- Trias J, Nikaido H: Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides. J Biol Chem. 1990 Sep 15;265(26):15680-4. [Article]
- Yoshihara E, Gotoh N, Nishino T, Nakae T: Protein D2 porin of the Pseudomonas aeruginosa outer membrane bears the protease activity. FEBS Lett. 1996 Sep 30;394(2):179-82. [Article]
- Yoshihara E, Yoneyama H, Ono T, Nakae T: Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1998 Jun 9;247(1):142-5. [Article]