Neopullulanase
Details
- Name
- Neopullulanase
- Synonyms
- 3.2.1.135
- Gene Name
- nplT
- Organism
- Geobacillus stearothermophilus
- Amino acid sequence
>lcl|BSEQ0012260|Neopullulanase MRKEAIYHRPADNFAYAYDSETLHLRLRTKKDDIDRVELLHGDPYDWQNGAWQFQMMPMR KTGSDELFDYWFAEVKPPYRRLRYGFVLYSGEEKLVYTEKGFYFEVPTDDTAYYFCFPFL HRVDLFEAPDWVKDTVWYQIFPERFANGNPSISPEGSRPWGSEDPTPTSFFGGDLQGIID HLDYLVDLGITGIYLTPIFRSPSNHKYDTADYFEVDPHFGDKETLKTLIDRCHEKGIRVM LDAVFNHCGYEFAPFQDVWKNGESSKYKDWFHIHEFPLQTEPRPNYDTFRFVPQMPKLNT ANPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHEFWREFRQEVKALKPDVYILGEIWH DAMPWLRGDQFDAVMNYPFTDGVLRFFAKEEISARQFANQMMHVLHSYPNNVNEAAFNLL GSHDTSRILTVCGGDIRKVKLLFLFQLTFTGSPCIYYGDEIGMTGGNDPECRKCMVWDPM QQNKELHQHVKQLIALRKQYRSLRRGEISFLHADDEMNYLIYKKTDGDETVLVIINRSDQ KADIPIPLDARGTWLVNLLTGERFAAEAETLCTSLPPYGFVLYAIEHW
- Number of residues
- 588
- Molecular Weight
- 69144.625
- Theoretical pI
- 5.09
- GO Classification
- Functionsmetal ion binding / neopullulanase activityProcessescarbohydrate metabolic process
- General Function
- Neopullulanase activity
- Specific Function
- Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0006322|1767 bp ATGAGGAAAGAAGCCATCTACCACCGCCCGGCTGACAACTTCGCCTATGCCTATGATAGT GAGACACTTCATCTTCGGCTTCGAACAAAAAAAGACGATATCGATCGTGTTGAGCTGCTG CATGGTGACCCGTATGACTGGCAAAATGGAGCTTGGCAGTTTCAAATGATGCCGATGCGA AAAACAGGAAGTGACGAGTTGTTTGATTATTGGTTCGCCGAAGTCAAACCTCCCTATCGC CGGTTACGCTACGGGTTCGTGCTGTATTCAGGAGAGGAGAAGCTCGTTTATACAGAAAAA GGGTTTTACTTTGAGGTTCCAACCGATGATACGGCTTACTACTTTTGCTTTCCCTTTCTT CATCGAGTCGACTTGTTCGAGGCGCCGGATTGGGTAAAGGATACAGTCTGGTATCAAATT TTCCCTGAGCGGTTCGCCAACGGCAACCCATCAATCAGTCCAGAAGGATCGCGGCCGTGG GGGAGCGAGGATCCAACACCAACCAGCTTTTTTGGCGGCGACTTGCAAGGGATTATCGAT CATCTCGATTACCTTGTTGACCTTGGTATTACCGGTATTTACTTAACGCCGATCTTCCGT TCTCCGTCAAACCATAAATACGATACCGCTGATTATTTTGAAGTCGATCCACACTTTGGG GATAAAGAAACGTTGAAAACGCTCATCGACCGTTGCCATGAAAAAGGTATCCGCGTTATG CTCGATGCCGTGTTTAACCATTGCGGCTATGAGTTCGCCCCGTTCCAAGATGTATGGAAA AATGGTGAGTCCTCAAAATATAAGGACTGGTTTCACATTCATGAATTTCCGCTGCAAACA GAGCCGCGGCCGAATTACGATACATTTCGATTCGTGCCACAAATGCCAAAGCTGAACACC GCCAATCCAGAAGTGAAGCGTTATTTGCTTGATGTTGCGACATATTGGATTCGTGAGTTT GACATTGACGGTTGGCGGCTTGATGTTGCCAATGAAATCGACCACGAATTTTGGCGCGAG TTCCGCCAGGAGGTAAAGGCACTGAAACCGGACGTATACATCCTCGGGGAAATTTGGCAT GATGCGATGCCGTGGCTGCGCGGTGACCAGTTTGACGCAGTCATGAACTACCCGTTTACA GACGGGGTGCTCCGCTTTTTCGCCAAGGAAGAGATTAGTGCACGCCAGTTTGCTAATCAA ATGATGCATGTACTTCATTCGTATCCGAACAATGTCAACGAGGCCGCATTCAATTTGCTC GGCAGTCATGATACATCAAGAATTCTCACCGTTTGCGGCGGGGATATCCGCAAGGTGAAG TTGTTATTTTTGTTTCAACTGACGTTCACGGGTTCACCATGCATTTACTATGGGGATGAA ATCGGCATGACGGGCGGAAACGATCCCGAGTGCCGGAAGTGCATGGTGTGGGATCCGATG CAACAAAACAAAGAGCTGCACCAACACGTCAAGCAGCTGATAGCGCTGCGCAAACAGTAT CGGTCACTACGCCGCGGGGAAATCTCCTTTCTTCATGCCGATGATGAAATGAACTATCTT ATTTACAAAAAAACAGATGGAGATGAAACGGTGTTAGTCATCATCAATCGGAGCGACCAA AAAGCCGACATCCCGATCCCGCTCGATGCAAGAGGAACATGGCTCGTTAACCTCTTGACT GGGGAACGGTTTGCAGCCGAGGCAGAAACGCTTTGCACCTCCTTACCGCCCTATGGGTTT GTACTTTATGCAATAGAACATTGGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P38940 UniProtKB Entry Name NEPU_GEOSE GenBank Gene ID M28138 - General References
- Kuriki T, Imanaka T: Nucleotide sequence of the neopullulanase gene from Bacillus stearothermophilus. J Gen Microbiol. 1989 Jun;135(6):1521-8. [Article]
- Hondoh H, Kuriki T, Matsuura Y: Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase. J Mol Biol. 2003 Feb 7;326(1):177-88. [Article]