Flavohemoprotein
Details
- Name
- Flavohemoprotein
- Synonyms
- fhp
- Flavohemoglobin
- Hemoglobin-like protein
- Nitric oxide dioxygenase
- NO oxygenase
- NOD
- Gene Name
- hmp
- Organism
- Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
- Amino acid sequence
>lcl|BSEQ0011214|Flavohemoprotein MLTQKTKDIVKATAPVLAEHGYDIIKCFYQRMFEAHPELKNVFNMAHQEQGQQQQALARA VYAYAENIEDPNSLMAVLKNIANKHASLGVKPEQYPIVGEHLLAAIKEVLGNAATDDIIS AWAQAYGNLADVLMGMESELYERSAEQPGGWKGWRTFVIREKRPESDVITSFILEPADGG PVVNFEPGQYTSVAIDVPALGLQQIRQYSLSDMPNGRSYRISVKREGGGPQPPGYVSNLL HDHVNVGDQVKLAAPYGSFHIDVDAKTPIVLISGGVGLTPMVSMLKVALQAPPRQVVFVH GARNSAVHAMRDRLREAAKTYENLDLFVFYDQPLPEDVQGRDYDYPGLVDVKQIEKSILL PDADYYICGPIPFMRMQHDALKNLGIHEARIHYEVFGPDLFAE
- Number of residues
- 403
- Molecular Weight
- 44781.75
- Theoretical pI
- 5.57
- GO Classification
- FunctionsFAD binding / heme binding / metal ion binding / nitric oxide dioxygenase activity / oxygen binding / oxygen transporter activityProcessesresponse to nitrosative stress / response to toxic substanceComponentscytoplasm
- General Function
- Oxygen transporter activity
- Specific Function
- Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.In the presence of oxygen and NADH, FHP has NADH oxidase activity, which leads to the generation of superoxide and H(2)O(2), both in vitro and in vivo, and it has been suggested that FHP might act as an amplifier of superoxide stress. Under anaerobic conditions, FHP also exhibits nitric oxide reductase and FAD reductase activities. However, all these reactions are much lower than NOD activity.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0011215|Flavohemoprotein (hmp) ATGCTGACCCAGAAAACAAAAGACATCGTAAAGGCCACCGCACCGGTGCTGGCCGAACAC GGCTACGACATCATCAAGTGCTTCTATCAGCGCATGTTCGAAGCGCATCCCGAACTGAAG AATGTGTTCAACATGGCCCACCAGGAACAGGGCCAGCAGCAGCAGGCGCTGGCACGGGCG GTCTATGCCTATGCGGAGAATATCGAGGACCCAAACAGTCTGATGGCCGTCCTGAAGAAC ATCGCCAACAAGCATGCAAGTCTCGGCGTCAAACCGGAACAGTACCCCATTGTTGGAGAA CACCTACTGGCCGCCATCAAGGAAGTGTTGGGCAATGCCGCAACCGACGACATCATTTCC GCTTGGGCGCAAGCCTACGGGAATCTGGCCGATGTCCTGATGGGCATGGAGAGCGAGTTG TATGAGCGCTCTGCGGAACAACCCGGTGGCTGGAAGGGGTGGCGCACCTTTGTGATTCGT GAGAAGCGCCCCGAGAGCGATGTGATCACGTCATTCATCCTTGAGCCTGCCGATGGTGGT CCCGTGGTGAACTTCGAACCCGGACAATACACCAGCGTCGCAATCGATGTGCCCGCGCTC GGGTTGCAGCAGATTCGCCAATACAGCCTGTCGGATATGCCGAACGGGCGCAGCTATCGC ATCTCGGTGAAGCGCGAGGGCGGCGGGCCGCAGCCTCCGGGCTATGTTTCGAACCTGTTG CATGATCACGTGAATGTTGGGGATCAGGTCAAGCTGGCTGCGCCCTATGGAAGCTTCCAT ATCGACGTTGACGCCAAGACACCCATTGTCCTGATCAGCGGTGGCGTTGGGCTGACACCG ATGGTCAGCATGCTCAAAGTGGCATTGCAGGCCCCACCGAGGCAAGTTGTATTCGTGCAT GGCGCACGTAACAGCGCAGTACACGCGATGCGCGACAGGCTGCGCGAAGCGGCGAAGACC TATGAAAACCTTGACCTCTTTGTTTTCTATGACCAACCGCTACCGGAAGATGTCCAGGGA CGGGACTATGATTATCCGGGCTTAGTCGATGTGAAGCAGATCGAGAAATCGATCCTGCTG CCAGACGCCGACTACTACATCTGCGGTCCGATTCCGTTCATGCGAATGCAGCATGATGCC CTGAAGAATCTCGGCATTCATGAGGCACGCATTCATTACGAAGTGTTTGGTCCCGACCTG TTTGCTGAGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P39662 UniProtKB Entry Name HMP_CUPNH GenBank Protein ID 460999 GenBank Gene ID X74334 - General References
- Cramm R, Siddiqui RA, Friedrich B: Primary sequence and evidence for a physiological function of the flavohemoprotein of Alcaligenes eutrophus. J Biol Chem. 1994 Mar 11;269(10):7349-54. [Article]
- Schwartz E, Henne A, Cramm R, Eitinger T, Friedrich B, Gottschalk G: Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based ithoautotrophy and anaerobiosis. J Mol Biol. 2003 Sep 12;332(2):369-83. [Article]
- Zhu H, Riggs AF: Yeast flavohemoglobin is an ancient protein related to globins and a reductase family. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5015-9. [Article]
- Probst I, Wolf G, Schlegel HG: An oxygen-binding flavohemoprotein from Alcaligenes eutrophus. Biochim Biophys Acta. 1979 Feb 26;576(2):471-8. [Article]
- Gardner PR, Gardner AM, Martin LA, Dou Y, Li T, Olson JS, Zhu H, Riggs AF: Nitric-oxide dioxygenase activity and function of flavohemoglobins. sensitivity to nitric oxide and carbon monoxide inhibition. J Biol Chem. 2000 Oct 13;275(41):31581-7. [Article]
- Ermler U, Siddiqui RA, Cramm R, Friedrich B: Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 A resolution. EMBO J. 1995 Dec 15;14(24):6067-77. [Article]
- Ollesch G, Kaunzinger A, Juchelka D, Schubert-Zsilavecz M, Ermler U: Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus. Eur J Biochem. 1999 Jun;262(2):396-405. [Article]