Mannan endo-1,4-beta-mannosidase

Details

Name
Mannan endo-1,4-beta-mannosidase
Synonyms
  • 3.2.1.78
  • man26A
  • ManA
  • Mannanase 26A
  • Mannanase A
Gene Name
manA
Organism
Cellvibrio japonicus (strain Ueda107)
Amino acid sequence
>lcl|BSEQ0011370|Mannan endo-1,4-beta-mannosidase
MKTITTARLPWAAQSFALGICLIALLGCNHAANKSSASRADVKPVTVKLVDSQATMETRS
LFAFMQEQRRHSIMFGHQHETTQGLTITRTDGTQSDTFNAVGDFAAVYGWDTLSIVAPKA
EGDIVAQVKKAYARGGIITVSSHFDNPKTDTQKGVWPVGTSWDQTPAVVDSLPGGAYNPV
LNGYLDQVAEWANNLKDEQGRLIPVIFRLYHENTGSWFWWGDKQSTPEQYKQLFRYSVEY
LRDVKGVRNFLYAYSPNNFWDVTEANYLERYPGDEWVDVLGFDTYGPVADNADWFRNVVA
NAALVARMAEARGKIPVISEIGIRAPDIEAGLYDNQWYRKLISGLKADPDAREIAFLLVW
RNAPQGVPGPNGTQVPHYWVPANRPENINNGTLEDFQAFYADEFTAFNRDIEQVYQRPTL
IVK
Number of residues
423
Molecular Weight
47486.99
Theoretical pI
5.46
GO Classification
Functions
mannan endo-1,4-beta-mannosidase activity
Processes
galactomannan metabolic process / glucomannan metabolic process / polysaccharide catabolic process
General Function
Mannan endo-1,4-beta-mannosidase activity
Specific Function
Catalyzes the endo hydrolysis of beta-1,4-linked mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall (PubMed:11382747). Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides (PubMed:7848261) (PubMed:8973192). It displays tight specificity for mannose at both the -2 and the -1 subsites (PubMed:19441796). Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan (PubMed:11064195).
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Gene sequence
>lcl|BSEQ0011371|Mannan endo-1,4-beta-mannosidase (manA)
ATGAAAACTATTACCACTGCACGCCTTCCATGGGCTGCGCAGAGCTTTGCCTTGGGCATT
TGCCTGATAGCGCTGTTGGGTTGTAATCACGCCGCTAATAAATCATCGGCATCCAGGGCA
GATGTCAAACCTGTTACGGTCAAACTGGTCGATTCCCAGGCGACCATGGAAACCCGTTCC
CTGTTTGCGTTTATGCAGGAGCAGCGCAGACATTCCATTATGTTTGGGCACCAGCACGAA
ACAACCCAGGGACTCACTATTACCCGCACAGATGGTACCCAATCCGATACCTTTAATGCC
GTTGGTGATTTTGCAGCGGTCTATGGTTGGGACACGCTGTCTATTGTTGCCCCTAAAGCG
GAGGGCGATATTGTCGCCCAAGTAAAAAAGGCTTATGCGCGCGGGGGCATCATTACGGTG
AGCTCGCATTTTGATAATCCAAAAACCGATACCCAAAAGGGCGTATGGCCCGTTGGCACT
TCCTGGGATCAAACCCCGGCTGTTGTGGATTCCCTGCCTGGAGGCGCCTATAACCCGGTA
TTAAATGGCTATCTCGACCAGGTGGCCGAGTGGGCCAATAACCTGAAAGACGAGCAGGGG
CGGCTGATACCGGTCATCTTTCGCCTGTACCACGAAAATACCGGCAGTTGGTTCTGGTGG
GGAGATAAGCAATCAACCCCTGAACAATACAAGCAATTGTTCCGCTATTCCGTCGAGTAT
TTGCGCGATGTGAAAGGTGTGCGCAATTTCCTGTATGCCTATTCGCCCAACAACTTTTGG
GACGTGACCGAGGCCAATTACCTGGAGCGTTATCCGGGGGATGAGTGGGTGGATGTACTG
GGATTTGATACCTATGGCCCGGTGGCGGACAACGCTGACTGGTTCCGCAATGTGGTGGCC
AATGCGGCCTTGGTTGCCCGCATGGCGGAAGCGCGGGGCAAAATACCGGTGATCTCCGAG
ATAGGTATACGCGCTCCCGACATTGAAGCCGGGCTCTATGATAACCAGTGGTATCGCAAG
CTTATCTCCGGCCTCAAGGCCGATCCGGATGCACGCGAGATTGCCTTCCTGCTGGTATGG
CGCAATGCCCCGCAGGGAGTTCCTGGCCCCAATGGCACCCAGGTTCCCCATTATTGGGTG
CCTGCTAACCGCCCGGAGAATATCAACAATGGCACCCTGGAGGACTTCCAGGCCTTTTAT
GCCGATGAATTCACAGCGTTCAATCGCGACATCGAGCAGGTCTATCAGCGTCCGACCCTG
ATAGTTAAATAG
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP49424
UniProtKB Entry NameMANA_CELJU
GenBank Protein ID14594858
GenBank Gene IDX82179
General References
  1. Braithwaite KL, Black GW, Hazlewood GP, Ali BR, Gilbert HJ: A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa. Biochem J. 1995 Feb 1;305 ( Pt 3):1005-10. [Article]
  2. DeBoy RT, Mongodin EF, Fouts DE, Tailford LE, Khouri H, Emerson JB, Mohamoud Y, Watkins K, Henrissat B, Gilbert HJ, Nelson KE: Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus. J Bacteriol. 2008 Aug;190(15):5455-63. doi: 10.1128/JB.01701-07. Epub 2008 Jun 13. [Article]
  3. Bolam DN, Hughes N, Virden R, Lakey JH, Hazlewood GP, Henrissat B, Braithwaite KL, Gilbert HJ: Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues. Biochemistry. 1996 Dec 17;35(50):16195-204. [Article]
  4. Halstead JR, Fransen MP, Eberhart RY, Park AJ, Gilbert HJ, Hazlewood GP: alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis. FEMS Microbiol Lett. 2000 Nov 15;192(2):197-203. [Article]
  5. Hogg D, Woo EJ, Bolam DN, McKie VA, Gilbert HJ, Pickersgill RW: Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding. J Biol Chem. 2001 Aug 17;276(33):31186-92. Epub 2001 May 29. [Article]
  6. Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabo L, Stoll D, Withers SG, Davies GJ: Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. [Article]
  7. Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG: Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chem Commun (Camb). 2003 Jun 21;(12):1327-9. [Article]
  8. Tailford LE, Ducros VM, Flint JE, Roberts SM, Morland C, Zechel DL, Smith N, Bjornvad ME, Borchert TV, Wilson KS, Davies GJ, Gilbert HJ: Understanding how diverse beta-mannanases recognize heterogeneous substrates. Biochemistry. 2009 Jul 28;48(29):7009-18. doi: 10.1021/bi900515d. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB026801,3-DinitrobenzeneexperimentalunknownDetails
DB038142-(N-morpholino)ethanesulfonic acidexperimentalunknownDetails
DB040842-deoxy-2-fluoro-α-D-mannoseexperimentalunknownDetails
DB044832-deoxy-2-fluoro-β-D-mannoseexperimentalunknownDetails