Ubiquitin carboxyl-terminal hydrolase 14

Details

Name
Ubiquitin carboxyl-terminal hydrolase 14
Synonyms
  • 3.4.19.12
  • Deubiquitinating enzyme 14
  • TGT
  • Ubiquitin thioesterase 14
  • Ubiquitin-specific-processing protease 14
Gene Name
USP14
UniProtKB Entry
P54578Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0049793|Ubiquitin carboxyl-terminal hydrolase 14
MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGN
IKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATV
QCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLH
MAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKK
SLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITK
QSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCT
PELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDL
QAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLY
GPRRVEIMEEESEQ
Number of residues
494
Molecular Weight
56068.665
Theoretical pI
Not Available
GO Classification
Functions
cysteine-type deubiquitinase activity / deubiquitinase activity / K63-linked deubiquitinase activity
Processes
innate immune response / negative regulation of ERAD pathway / negative regulation of ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process
Components
cytoplasm
General Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029)
Specific Function
Cysteine-type deubiquitinase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0049794|Ubiquitin carboxyl-terminal hydrolase 14 (USP14)
ATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATTTGAAGGTGTAGAATTG
AATACAGATGAACCTCCAATGGTATTCAAGGCTCAGCTGTTTGCGTTGACTGGAGTCCAG
CCTGCCAGACAGAAAGTTATGGTGAAAGGAGGAACGCTAAAGGATGATGATTGGGGAAAC
ATCAAAATAAAAAATGGAATGACTCTACTAATGATGGGGTCAGCAGATGCTCTTCCAGAA
GAACCCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAGAAGAACAGTTAGCATCTGCT
ATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTACATGAATGCCACAGTT
CAGTGTATTCGTTCTGTGCCTGAACTCAAAGATGCCCTTAAAAGGTATGCAGGTGCCTTG
AGAGCTTCAGGGGAAATGGCTTCAGCGCAGTATATTACTGCAGCCCTTAGAGATTTGTTT
GATTCCATGGATAAAACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGCAC
ATGGCTTTCCCACAGTTTGCCGAGAAAGGTGAACAAGGACAGTATCTTCAACAGGATGCT
AATGAATGTTGGATACAAATGATGCGAGTATTGCAACAGAAATTGGAAGCAATAGAGGAT
GATTCTGTTAAAGAGACAGACTCCTCATCTGCATCGGCAGCGACACCTTCTAAAAAGAAA
AGTTTAATCGATCAGTTCTTCGGTGTTGAGTTTGAAACTACCATGAAATGTACAGAATCT
GAAGAAGAAGAAGTCACCAAAGGAAAGGAAAATCAACTTCAGCTTAGCTGTTTTATCAAT
CAGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAAGAAATCACCAAA
CAGTCTCCAACGTTGCAAAGAAATGCCTTGTATATCAAATCTTCCAAGATCAGCCGGCTG
CCTGCTTACTTGACCATTCAGATGGTTCGATTTTTTTATAAAGAGAAGGAATCTGTGAAT
GCCAAAGTTCTTAAGGATGTTAAATTTCCTCTTATGTTGGATATGTATGAACTGTGTACA
CCAGAACTTCAAGAGAAAATGGTGTCTTTTCGATCCAAATTCAAGGATCTAGAAGATAAA
AAAGTGAATCAGCAGCCAAATACAAGTGACAAAAAGAGTAGTCCCCAGAAAGAAGTTAAG
TATGAACCCTTTTCTTTTGCTGATGATATTGGCTCCAATAATTGTGGATACTATGACTTA
CAAGCAGTACTAACACACCAGGGAAGGTCTAGTTCTTCAGGTCATTATGTATCATGGGTG
AAAAGGAAACAAGATGAATGGATTAAGTTTGATGATGACAAAGTCAGCATCGTAACACCA
GAAGATATCTTACGGCTTTCTGGTGGTGGAGACTGGCATATCGCTTACGTTCTACTCTAT
GGGCCTCGCAGAGTTGAAATAATGGAAGAGGAAAGTGAACAGTAA
Chromosome Location
18
Locus
18p11.32
External Identifiers
ResourceLink
UniProtKB IDP54578
UniProtKB Entry NameUBP14_HUMAN
GeneCard IDUSP14
HGNC IDHGNC:12612
PDB ID(s)2AYN, 2AYO, 5GJQ, 6IIK, 6IIL, 6IIM, 6IIN, 6LVS, 7W37, 7W38, 7W39, 7W3A, 7W3B, 7W3C, 7W3F, 7W3G, 7W3H, 7W3I, 7W3J, 7W3K, 7W3M
KEGG IDhsa:9097
IUPHAR/Guide To Pharmacology ID2429
NCBI Gene ID9097
General References
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Associated Data

Bio-Entities
Bio-EntityType
Ubiquitin carboxyl-terminal hydrolase 14 (Humans)protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Phenethyl IsothiocyanateinvestigationalunknowntargetDetails