Maltose-6'-phosphate glucosidase
Details
- Name
- Maltose-6'-phosphate glucosidase
- Synonyms
- 3.2.1.122
- 6-phospho-alpha-D-glucosidase
- 6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
- glv-1
- glvG
- malA
- Gene Name
- glvA
- Organism
- Bacillus subtilis (strain 168)
- Amino acid sequence
>lcl|BSEQ0016381|Maltose-6'-phosphate glucosidase MKKKSFSIVIAGGGSTFTPGIVLMLLDHLEEFPIRKLKLYDNDKERQDRIAGACDVFIRE KAPDIEFAATTDPEEAFTDVDFVMAHIRVGKYAMRALDEQIPLKYGVVGQETCGPGGIAY GMRSIGGVLEILDYMEKYSPDAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPVGIEDR MAQILGLSSRKEMKVRYYGLNHFGWWTSIQDQEGNDLMPKLKEHVSQYGYIPKTEAEAVE ASWNDTFAKARDVQAADPDTLPNTYLQYYLFPDDMVKKSNPNHTRANEVMEGREAFIFSQ CDMITREQSSENSEIKIDDHASYIVDLARAIAYNTGERMLLIVENNGAIANFDPTAMVEV PCIVGSNGPEPITVGTIPQFQKGLMEQQVSVEKLTVEAWAEKSFQKLWQALILSKTVPNA RVARLILEDLVEANKDFWPELDQSPTRIS
- Number of residues
- 449
- Molecular Weight
- 50513.14
- Theoretical pI
- 4.67
- GO Classification
- Functionsmaltose-6'-phosphate glucosidase activity / metal ion binding / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptorProcessescarbohydrate metabolic process
- General Function
- Oxidoreductase activity, acting on the ch-oh group of donors, nad or nadp as acceptor
- Specific Function
- Hydrolyzes maltose-6'-phosphate and trehalose-6'-phosphate. Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system (PEP-PTS). Is also able to significantly catalyze the hydrolysis of both 6-phospho-alpha- and 6-phospho-beta-glucosides containing activated leaving groups such as p-nitrophenol and does so with retention and inversion, respectively, of the substrate anomeric configuration.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0016382|Maltose-6'-phosphate glucosidase (glvA) ATGAAGAAAAAATCATTCTCAATCGTAATAGCGGGCGGAGGGAGCACTTTCACTCCAGGG ATCGTACTCATGCTCTTGGACCATTTGGAGGAGTTTCCGATCAGAAAGCTGAAGCTGTAT GATAATGATAAGGAGAGACAGGATCGAATTGCAGGCGCCTGTGACGTTTTTATCAGAGAA AAAGCGCCGGATATTGAATTTGCAGCGACGACTGACCCGGAAGAAGCTTTTACAGATGTC GATTTTGTTATGGCGCACATCAGAGTAGGGAAATACGCGATGCGCGCGCTTGATGAGCAA ATTCCGTTAAAGTACGGAGTTGTCGGCCAGGAGACGTGCGGGCCGGGCGGGATCGCATAC GGTATGCGTTCGATCGGCGGTGTGCTTGAAATATTAGATTACATGGAAAAATACTCGCCT GATGCGTGGATGCTCAATTATTCCAATCCGGCGGCAATTGTGGCTGAAGCTACGAGACGC CTTAGACCGAATTCTAAAATTCTCAATATCTGTGATATGCCGGTTGGGATCGAAGACCGG ATGGCGCAAATTCTTGGCTTATCCTCAAGAAAAGAAATGAAGGTCCGCTATTACGGATTG AATCATTTCGGCTGGTGGACATCGATTCAGGATCAAGAGGGCAACGATTTAATGCCGAAG CTGAAGGAACATGTATCCCAATACGGTTATATTCCGAAAACAGAGGCTGAAGCTGTGGAG GCAAGCTGGAATGACACGTTCGCCAAAGCGCGTGACGTGCAGGCTGCAGATCCTGACACA CTGCCGAATACGTATTTGCAATATTATTTGTTCCCAGATGATATGGTGAAAAAATCAAAT CCGAATCATACGCGGGCGAATGAAGTCATGGAAGGGCGCGAAGCTTTTATTTTCAGCCAA TGTGACATGATCACACGTGAACAGTCCTCGGAAAACAGCGAAATTAAAATCGATGACCAC GCATCGTATATCGTTGATCTTGCCCGGGCGATTGCCTACAACACAGGTGAAAGAATGCTG TTGATTGTTGAAAATAACGGTGCAATTGCGAACTTTGACCCGACTGCGATGGTTGAGGTG CCATGCATCGTCGGCTCAAATGGACCTGAACCGATTACCGTTGGCACCATTCCGCAATTC CAGAAAGGGCTCATGGAGCAGCAGGTATCCGTTGAGAAGCTGACTGTTGAAGCGTGGGCA GAGAAATCGTTCCAAAAGCTGTGGCAGGCGCTGATCCTGTCAAAAACAGTGCCGAACGCG CGTGTGGCAAGACTCATTCTTGAGGATTTAGTGGAGGCCAACAAAGACTTCTGGCCTGAG CTTGATCAAAGCCCAACCCGCATATCATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P54716 UniProtKB Entry Name GLVA_BACSU GenBank Protein ID 1486241 GenBank Gene ID D50543 - General References
- Yamamoto H, Uchiyama S, Fajar AN, Ogasawara N, Sekiguchi J: Determination of a 12 kb nucleotide sequence around the 76 degrees region of the Bacillus subtilis chromosome. Microbiology. 1996 Jun;142 ( Pt 6):1417-21. [Article]
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- Thompson J, Pikis A, Ruvinov SB, Henrissat B, Yamamoto H, Sekiguchi J: The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily. J Biol Chem. 1998 Oct 16;273(42):27347-56. [Article]
- Yamamoto H, Serizawa M, Thompson J, Sekiguchi J: Regulation of the glv operon in Bacillus subtilis: YfiA (GlvR) is a positive regulator of the operon that is repressed through CcpA and cre. J Bacteriol. 2001 Sep;183(17):5110-21. [Article]
- Varrot A, Yamamoto H, Sekiguchi J, Thompson J, Davies GJ: Crystallization and preliminary X-ray analysis of the 6-phospho-alpha-glucosidase from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1212-4. [Article]
- Yip VL, Thompson J, Withers SG: Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4. Biochemistry. 2007 Aug 28;46(34):9840-52. Epub 2007 Aug 4. [Article]
- Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF: Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure. 2004 Sep;12(9):1619-29. [Article]