Bifunctional protein MdtA

Details

Name

Bifunctional protein MdtA

Synonyms

• 1.5.1.-

Gene Name

mtdA

Organism

Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1)

Amino acid sequence

>lcl|BSEQ0017002|Bifunctional protein MdtA
MSKKLLFQFDTDATPSVFDVVVGYDGGADHITGYGNVTPDNVGAYVDGTIYTRGGKEKQS
TAIFVGGGDMAAGERVFEAVKKRFFGPFRVSCMLDSNGSNTTAAAGVALVVKAAGGSVKG
KKAVVLAGTGPVGMRSAALLAGEGAEVVLCGRKLDKAQAAADSVNKRFKVNVTAAETADD
ASRAEAVKGAHFVFTAGAIGLELLPQAAWQNESSIEIVADYNAQPPLGIGGIDATDKGKE
YGGKRAFGALGIGGLKLKLHRACIAKLFESSEGVFDAEEIYKLAKEMA

Number of residues

288

Molecular Weight

29735.595

Theoretical pI

7.43

GO Classification

Functions

methylenetetrahydrofolate dehydrogenase (NADP+) activity

Processes

formaldehyde catabolic process / one-carbon metabolic process

Components

cytoplasm

General Function

Methylenetetrahydrofolate dehydrogenase (nadp+) activity

Specific Function

Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency.

Pfam Domain Function

• Mpt_N (PF09176)

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0017003|Bifunctional protein MdtA (mtdA)
ATGTCCAAGAAGCTGCTCTTCCAGTTCGACACCGATGCCACGCCGAGCGTCTTCGACGTC
GTCGTCGGCTACGACGGCGGTGCCGACCACATCACCGGCTACGGCAACGTCACGCCCGAC
AACGTCGGCGCCTATGTCGACGGCACGATCTACACCCGCGGCGGCAAGGAGAAGCAGTCG
ACGGCGATCTTCGTCGGCGGCGGCGACATGGCGGCCGGCGAGCGGGTGTTCGAGGCGGTG
AAGAAGCGCTTCTTCGGCCCGTTCCGCGTGTCCTGCATGCTGGATTCGAACGGCTCCAAC
ACGACCGCCGCGGCGGGTGTGGCGCTCGTCGTCAAGGCGGCGGGCGGCTCGGTCAAGGGC
AAGAAGGCCGTCGTGCTCGCGGGCACCGGCCCGGTCGGCATGCGCTCGGCGGCGCTGCTC
GCCGGCGAGGGCGCCGAGGTCGTGCTGTGCGGGCGCAAGCTCGACAAGGCGCAGGCCGCG
GCCGATTCCGTGAACAAGCGCTTCAAGGTGAACGTCACCGCGGCCGAAACCGCGGACGAC
GCTTCGCGCGCCGAGGCCGTGAAGGGCGCCCATTTCGTCTTCACCGCCGGTGCGATCGGC
CTTGAACTGCTGCCGCAGGCAGCCTGGCAGAACGAGAGTTCGATCGAGATCGTGGCCGAC
TACAACGCCCAGCCGCCGCTCGGCATCGGCGGGATCGATGCGACCGACAAAGGCAAGGAA
TACGGCGGAAAGCGCGCCTTCGGTGCGCTCGGCATCGGCGGCTTGAAGCTCAAGCTGCAC
CGCGCCTGCATCGCCAAGCTGTTCGAGTCGAGCGAAGGCGTCTTCGACGCCGAGGAGATC
TACAAGCTGGCCAAGGAAATGGCCTGA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P55818
UniProtKB Entry Name	MTDA_METEA

General References

1. Chistoserdova LV, Lidstrom ME: Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA. J Bacteriol. 1994 Apr;176(7):1957-68. [Article]
2. Vuilleumier S, Chistoserdova L, Lee MC, Bringel F, Lajus A, Zhou Y, Gourion B, Barbe V, Chang J, Cruveiller S, Dossat C, Gillett W, Gruffaz C, Haugen E, Hourcade E, Levy R, Mangenot S, Muller E, Nadalig T, Pagni M, Penny C, Peyraud R, Robinson DG, Roche D, Rouy Z, Saenampechek C, Salvignol G, Vallenet D, Wu Z, Marx CJ, Vorholt JA, Olson MV, Kaul R, Weissenbach J, Medigue C, Lidstrom ME: Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS One. 2009;4(5):e5584. doi: 10.1371/journal.pone.0005584. Epub 2009 May 18. [Article]
3. Vorholt JA, Chistoserdova L, Lidstrom ME, Thauer RK: The NADP-dependent methylene tetrahydromethanopterin dehydrogenase in Methylobacterium extorquens AM1. J Bacteriol. 1998 Oct;180(20):5351-6. [Article]
4. Hagemeier CH, Bartoschek S, Griesinger C, Thauer RK, Vorholt JA: Re-face stereospecificity of NADP dependent methylenetetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 as determined by NMR spectroscopy. FEBS Lett. 2001 Apr 6;494(1-2):95-8. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB03461	Nicotinamide adenine dinucleotide phosphate	experimental	unknown		Details