50S ribosomal protein L4


50S ribosomal protein L4
  • eryA
  • Large ribosomal subunit protein uL4
Gene Name
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0019455|50S ribosomal protein L4
Number of residues
Molecular Weight
Theoretical pI
GO Classification
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding / endoribonuclease inhibitor activity / RNA binding / rRNA binding / structural constituent of ribosome / translation repressor activity
DNA-templated transcription, termination / negative regulation of endoribonuclease activity / negative regulation of transcription, DNA-templated / negative regulation of translation / response to antibiotic / ribosome assembly / translation
cytosol / cytosolic large ribosomal subunit / large ribosomal subunit / polysomal ribosome / protein-DNA complex
General Function
One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA (PubMed:3298242). It is important during the early stages of 50S assembly (PubMed:3298242). It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (PubMed:7556101, PubMed:6170935).
Specific Function
Bacterial-type rna polymerase transcriptional repressor activity, sequence-specific dna binding
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0019456|50S ribosomal protein L4 (rplD)
Chromosome Location
Not Available
Not Available
External Identifiers
UniProtKB IDP60723
UniProtKB Entry NameRL4_ECOLI
GenBank Protein ID42826
GenBank Gene IDX02613
General References
  1. Zurawski G, Zurawski SM: Structure of the Escherichia coli S10 ribosomal protein operon. Nucleic Acids Res. 1985 Jun 25;13(12):4521-6. [Article]
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  4. Link AJ, Robison K, Church GM: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis. 1997 Aug;18(8):1259-313. [Article]
  5. Urlaub H, Kruft V, Bischof O, Muller EC, Wittmann-Liebold B: Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies. EMBO J. 1995 Sep 15;14(18):4578-88. [Article]
  6. Wower I, Wower J, Meinke M, Brimacombe R: The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29. Nucleic Acids Res. 1981 Sep 11;9(17):4285-302. [Article]
  7. VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
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  9. Wittmann HG, Stoffler G, Apirion D, Rosen L, Tanaka K, Tamaki M, Takata R, Dekio S, Otaka E: Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1973 Dec 20;127(2):175-89. [Article]
  10. Herold M, Nierhaus KH: Incorporation of six additional proteins to complete the assembly map of the 50 S subunit from Escherichia coli ribosomes. J Biol Chem. 1987 Jun 25;262(18):8826-33. [Article]
  11. Freedman LP, Zengel JM, Archer RH, Lindahl L: Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6516-20. [Article]
  12. Zengel JM, Lindahl L: Escherichia coli ribosomal protein L4 stimulates transcription termination at a specific site in the leader of the S10 operon independent of L4-mediated inhibition of translation. J Mol Biol. 1990 May 5;213(1):67-78. [Article]
  13. Zengel JM, Lindahl L: Ribosomal protein L4 stimulates in vitro termination of transcription at a NusA-dependent terminator in the S10 operon leader. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2675-9. [Article]
  14. Chittum HS, Champney WS: Ribosomal protein gene sequence changes in erythromycin-resistant mutants of Escherichia coli. J Bacteriol. 1994 Oct;176(20):6192-8. [Article]
  15. Chittum HS, Champney WS: Erythromycin inhibits the assembly of the large ribosomal subunit in growing Escherichia coli cells. Curr Microbiol. 1995 May;30(5):273-9. [Article]
  16. Li X, Lindahl L, Zengel JM: Ribosomal protein L4 from Escherichia coli utilizes nonidentical determinants for its structural and regulatory functions. RNA. 1996 Jan;2(1):24-37. [Article]
  17. Allen T, Shen P, Samsel L, Liu R, Lindahl L, Zengel JM: Phylogenetic analysis of L4-mediated autogenous control of the S10 ribosomal protein operon. J Bacteriol. 1999 Oct;181(19):6124-32. [Article]
  18. Zengel JM, Jerauld A, Walker A, Wahl MC, Lindahl L: The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control. RNA. 2003 Oct;9(10):1188-97. [Article]
  19. Arnold RJ, Reilly JP: Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry. Anal Biochem. 1999 Apr 10;269(1):105-12. [Article]
  20. Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J: Study of the structural dynamics of the E coli 70S ribosome using real-space refinement. Cell. 2003 Jun 13;113(6):789-801. [Article]
  21. Gabashvili IS, Gregory ST, Valle M, Grassucci R, Worbs M, Wahl MC, Dahlberg AE, Frank J: The polypeptide tunnel system in the ribosome and its gating in erythromycin resistance mutants of L4 and L22. Mol Cell. 2001 Jul;8(1):181-8. [Article]
  22. Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH: Structures of the bacterial ribosome at 3.5 A resolution. Science. 2005 Nov 4;310(5749):827-34. [Article]
  23. Bischoff L, Berninghausen O, Beckmann R: Molecular basis for the ribosome functioning as an L-tryptophan sensor. Cell Rep. 2014 Oct 23;9(2):469-75. doi: 10.1016/j.celrep.2014.09.011. Epub 2014 Oct 9. [Article]
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Drug Relations

Drug Relations
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