ADP-L-glycero-D-manno-heptose-6-epimerase
Details
- Name
- ADP-L-glycero-D-manno-heptose-6-epimerase
- Synonyms
- 5.1.3.20
- ADP-glyceromanno-heptose 6-epimerase
- ADP-hep 6-epimerase
- ADP-L-glycero-beta-D-manno-heptose-6-epimerase
- AGME
- htrM
- rfaD
- waaD
- Gene Name
- hldD
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0010904|ADP-L-glycero-D-manno-heptose-6-epimerase MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVDLNIADYMDKEDFLIQI MAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLHYCLEREIPFLYASSAATY GGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANSQIVGFRYFNVYGPREGHKGS MASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVADVNLWFLENGVSGIFNLGTGRA ESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFTQADLTNLRAAGYDKPFKTVAEGVT EYMAWLNRDA
- Number of residues
- 310
- Molecular Weight
- 34892.905
- Theoretical pI
- 4.54
- GO Classification
- FunctionsADP-glyceromanno-heptose 6-epimerase activity / NADP+ bindingProcessesADP-L-glycero-beta-D-manno-heptose biosynthetic process / lipopolysaccharide core region biosynthetic processComponentscytosol / membrane
- General Function
- Nadp+ binding
- Specific Function
- Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose.
- Pfam Domain Function
- Epimerase (PF01370)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0010905|ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) ATGATCATCGTTACCGGCGGCGCGGGCTTTATCGGCAGCAACATCGTTAAAGCCCTGAAT GATAAAGGCATCACCGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTG AACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATT ATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCC ACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTG CTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTAC GGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTAC GGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCG CAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGC ATGGCGAGCGTCGCTTTCCATCTCAACACCCAGCTTAACAACGGTGAATCACCGAAGCTG TTTGAAGGTAGCGAGAACTTCAAACGCGACTTCGTCTATGTGGGCGACGTGGCTGATGTG AATCTGTGGTTCCTGGAAAATGGCGTTTCCGGCATCTTCAATCTCGGTACTGGTCGTGCG GAATCCTTCCAGGCGGTAGCTGATGCTACGCTGGCTTATCACAAGAAAGGCCAGATCGAA TACATTCCGTTCCCGGATAAACTGAAAGGCCGCTATCAGGCGTTCACTCAGGCAGATCTG ACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACG GAATACATGGCCTGGCTGAATCGCGACGCATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P67910 UniProtKB Entry Name HLDD_ECOLI GenBank Protein ID 147576 GenBank Gene ID M33577 - General References
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- Raina S, Georgopoulos C: The htrM gene, whose product is essential for Escherichia coli viability only at elevated temperatures, is identical to the rfaD gene. Nucleic Acids Res. 1991 Jul 25;19(14):3811-9. [Article]
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- Coleman WG Jr: The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis. J Biol Chem. 1983 Feb 10;258(3):1985-90. [Article]
- Ding L, Zhang Y, Deacon AM, Ealick SE, Ni Y, Sun P, Coleman WG Jr: Crystallization and preliminary X-ray diffraction studies of the lipopolysaccharide core biosynthetic enzyme ADP-L-glycero-D-mannoheptose 6-epimerase from Escherichia coli K-12. Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):685-8. [Article]
- Ni Y, McPhie P, Deacon A, Ealick S, Coleman WG Jr: Evidence that NADP+ is the physiological cofactor of ADP-L-glycero-D-mannoheptose 6-epimerase. J Biol Chem. 2001 Jul 20;276(29):27329-34. Epub 2001 Apr 19. [Article]
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- Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y: Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli. Mol Cell Proteomics. 2009 Feb;8(2):215-25. doi: 10.1074/mcp.M800187-MCP200. Epub 2008 Aug 23. [Article]
- Deacon AM, Ni YS, Coleman WG Jr, Ealick SE: The crystal structure of ADP-L-glycero-D-mannoheptose 6-epimerase: catalysis with a twist. Structure. 2000 May 15;8(5):453-62. [Article]