N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component

Details

Name
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component
Synonyms
  • 2.7.1.69
  • celA
  • PTS system N,N'-diacetylchitobiose-specific EIIB component
Gene Name
chbB
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016567|N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component
MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLAGEKGQNADVVLLGPQI
AYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKKAAAN
Number of residues
106
Molecular Weight
11426.435
Theoretical pI
8.45
GO Classification
Functions
kinase activity / protein-N(PI)-phosphohistidine-sugar phosphotransferase activity / protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity
Processes
carbohydrate transmembrane transport / N,N'-diacetylchitobiose import / phosphoenolpyruvate-dependent sugar phosphotransferase system
Components
cytosol
General Function
Protein-phosphocysteine-n,n'-diacetylchitobiose phosphotransferase system transporter activity
Specific Function
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0016568|N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component (chbB)
ATGGAAAAGAAACACATTTATCTGTTTTGTTCTGCGGGCATGTCTACCTCTTTACTGGTA
TCAAAAATGCGCGCACAGGCAGAAAAATATGAAGTTCCGGTCATTATTGAAGCATTTCCG
GAAACACTGGCTGGTGAAAAAGGTCAGAATGCCGATGTCGTGTTATTAGGGCCGCAGATT
GCTTATATGTTGCCCGAAATCCAGCGTTTGTTACCCAACAAACCGGTTGAAGTAATTGAC
TCGCTGCTTTATGGCAAAGTCGATGGTTTAGGCGTGCTTAAGGCTGCGGTTGCAGCGATT
AAAAAAGCCGCAGCAAATTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP69795
UniProtKB Entry NamePTQB_ECOLI
GenBank Protein ID41097
GenBank Gene IDX52890
General References
  1. Parker LL, Hall BG: Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12. Genetics. 1990 Mar;124(3):455-71. [Article]
  2. Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Reizer J, Reizer A, Saier MH Jr: The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus. Res Microbiol. 1990 Nov-Dec;141(9):1061-7. [Article]
  6. Keyhani NO, Roseman S: Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14367-71. [Article]
  7. Keyhani NO, Wang LX, Lee YC, Roseman S: The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system. J Biol Chem. 2000 Oct 20;275(42):33084-90. [Article]
  8. Keyhani NO, Bacia K, Roseman S: The transport/phosphorylation of N,N'-diacetylchitobiose in Escherichia coli. Characterization of phospho-IIB(Chb) and of a potential transition state analogue in the phosphotransfer reaction between the proteins IIA(Chb) AND IIB(Chb). J Biol Chem. 2000 Oct 20;275(42):33102-9. [Article]
  9. Keyhani N, Rodgers ME, Demeler B, Hansen JC, Roseman S: Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex. J Biol Chem. 2000 Oct 20;275(42):33110-5. [Article]
  10. Ab E, Schuurman-Wolters GK, Saier MH, Reizer J, Jacuinod M, Roepstorff P, Dijkstra K, Scheek RM, Robillard GT: Enzyme IIBcellobiose of the phosphoenol-pyruvate-dependent phosphotransferase system of Escherichia coli: backbone assignment and secondary structure determined by three-dimensional NMR spectroscopy. Protein Sci. 1994 Feb;3(2):282-90. [Article]
  11. Ab E, Schuurman-Wolters G, Reizer J, Saier MH, Dijkstra K, Scheek RM, Robillard GT: The NMR side-chain assignments and solution structure of enzyme IIBcellobiose of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. Protein Sci. 1997 Feb;6(2):304-14. [Article]
  12. van Montfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Thunnissen MM, Robillard GT, Dijkstra BW: The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases. Structure. 1997 Feb 15;5(2):217-25. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03544S-PhosphocysteineexperimentalunknownDetails