Mycothiol acetyltransferase
Details
- Name
- Mycothiol acetyltransferase
- Synonyms
- 2.3.1.189
- MSH acetyltransferase
- Mycothiol synthase
- Gene Name
- mshD
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- Amino acid sequence
>lcl|BSEQ0051157|Mycothiol acetyltransferase MTALDWRSALTADEQRSVRALVTATTAVDGVAPVGEQVLRELGQQRTEHLLVAGSRPGGP IIGYLNLSPPRGAGGAMAELVVHPQSRRRGIGTAMARAALAKTAGRNQFWAHGTLDPARA TASALGLVGVRELIQMRRPLRDIPEPTIPDGVVIRTYAGTSDDAELLRVNNAAFAGHPEQ GGWTAVQLAERRGEAWFDPDGLILAFGDSPRERPGRLLGFHWTKVHPDHPGLGEVYVLGV DPAAQRRGLGQMLTSIGIVSLARRLGGRKTLDPAVEPAVLLYVESDNVAAVRTYQSLGFT TYSVDTAYALAGTDN
- Number of residues
- 315
- Molecular Weight
- 33598.82
- Theoretical pI
- Not Available
- GO Classification
- Functionsmycothiol synthase activity / N-acetyltransferase activityProcessesgrowth of symbiont in host cell / mycothiol biosynthetic process / response to stressComponentscytosol
- General Function
- Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.
- Specific Function
- Mycothiol synthase activity
- Pfam Domain Function
- Acetyltransf_1 (PF00583)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0051158|Mycothiol acetyltransferase (mshD) GTGACGGCGCTTGACTGGCGCTCCGCTCTGACCGCCGACGAGCAGCGCAGCGTGCGTGCA CTGGTCACGGCGACAACAGCAGTCGATGGGGTAGCACCCGTGGGTGAACAGGTGCTGCGG GAACTGGGCCAGCAACGCACCGAGCATCTGCTGGTGGCCGGTTCGCGACCGGGCGGCCCG ATCATCGGCTACCTCAACCTCAGCCCACCCCGGGGCGCGGGTGGTGCGATGGCGGAGTTG GTGGTGCATCCGCAGTCTCGACGGCGCGGTATCGGCACCGCCATGGCCCGCGCGGCATTG GCCAAGACCGCCGGCCGCAACCAGTTCTGGGCGCACGGCACGCTGGATCCCGCTCGGGCG ACCGCGTCCGCGCTGGGTCTGGTCGGCGTCCGCGAACTGATCCAGATGCGACGCCCGCTG CGTGATATCCCCGAACCGACGATCCCCGACGGGGTGGTGATCCGCACCTACGCGGGCACG TCCGACGACGCTGAGCTACTCCGGGTCAACAACGCCGCGTTCGCCGGACACCCGGAACAG GGTGGGTGGACCGCGGTCCAGCTTGCCGAGCGGCGTGGCGAGGCGTGGTTCGATCCAGAC GGCCTGATCTTGGCCTTCGGTGATTCGCCACGTGAACGGCCTGGCCGGTTGCTGGGTTTC CATTGGACCAAAGTGCATCCCGATCACCCGGGATTGGGCGAGGTGTACGTGCTGGGCGTC GATCCGGCGGCGCAGCGCCGCGGTCTCGGCCAGATGTTGACGTCGATCGGTATCGTCTCG CTGGCCCGTCGGCTGGGCGGTCGGAAGACCCTCGACCCTGCGGTCGAACCCGCCGTGCTG CTCTACGTGGAGTCGGACAATGTGGCGGCCGTGCGAACCTACCAGAGCCTGGGCTTCACC ACCTACAGCGTCGATACCGCCTACGCGCTGGCTGGCACGGATAACTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WJM7 UniProtKB Entry Name MSHD_MYCTU - General References
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Koledin T, Newton GL, Fahey RC: Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes. Arch Microbiol. 2002 Nov;178(5):331-7. Epub 2002 Aug 15. [Article]
- Raman K, Yeturu K, Chandra N: targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis. BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Vetting MW, Roderick SL, Yu M, Blanchard JS: Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases. Protein Sci. 2003 Sep;12(9):1954-9. [Article]
- Vetting MW, Yu M, Rendle PM, Blanchard JS: The substrate-induced conformational change of Mycobacterium tuberculosis mycothiol synthase. J Biol Chem. 2006 Feb 3;281(5):2795-802. Epub 2005 Dec 2. [Article]