Carminomycin 4-O-methyltransferase DnrK
Details
- Name
- Carminomycin 4-O-methyltransferase DnrK
- Synonyms
- 2.1.1.292
- Anthracycline 4-O-methyltransferase
- COMT
- Gene Name
- dnrK
- UniProtKB Entry
- Q06528Swiss-Prot
- Organism
- Streptomyces peucetius
- NCBI Taxonomy ID
- 1950
- Amino acid sequence
>lcl|BSEQ0021978|Carminomycin 4-O-methyltransferase DnrK MTAEPTVAARPQQIDALRTLIRLGSLHTPMVVRTAATLRLVDHILAGARTVKALAARTDT RPEALLRLIRHLVAIGLLEEDAPGEFVPTEVGELLADDHPAAQRAWHDLTQAVARADISF TRLPDAIRTGRPTYESIYGKPFYEDLAGRPDLRASFDSLLACDQDVAFDAPAAAYDWTNV RHVLDVGGGKGGFAAAIARRAPHVSATVLEMAGTVDTARSYLKDEGLSDRVDVVEGDFFE PLPRKADAIILSFVLLNWPDHDAVRILTRCAEALEPGGRILIHERDDLHENSFNEQFTEL DLRMLVFLGGALRTREKWDGLAASAGLVVEEVRQLPSPTIPYDLSLLVLAPAATGA
- Number of residues
- 356
- Molecular Weight
- 38781.615
- Theoretical pI
- 4.95
- GO Classification
- Functionsmethyltransferase activity / O-methyltransferase activityProcessesdaunorubicin biosynthetic process
- General Function
- Not Available
- Specific Function
- Not Available
- Pfam Domain Function
- Methyltransf_2 (PF00891)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0003394|1071 bp ATGACAGCCGAACCGACGGTCGCGGCCCGGCCGCAGCAGATCGACGCCCTCAGGACCCTG ATCCGCCTCGGAAGCCTGCACACGCCCATGGTCGTCCGGACGGCCGCCACCCTGCGGCTC GTCGACCACATCCTGGCCGGGGCCCGCACCGTGAAGGCCCTGGCGGCCAGGACAGACACC CGGCCGGAAGCACTCCTGCGACTGATCCGCCACCTGGTGGCGATCGGACTGCTCGAGGAG GACGCACCGGGCGAGTTCGTCCCGACCGAGGTCGGCGAGCTGCTCGCCGACGACCACCCA GCCGCGCAGCGTGCCTGGCACGACCTGACGCAGGCCGTGGCGCGCGCCGACATCTCCTTC ACCCGCCTCCCCGACGCCATCCGTACCGGCCGCCCCACGTACGAGTCCATCTACGGCAAG CCGTTCTACGAGGACCTGGCCGGCCGCCCCGACCTGCGCGCGTCCTTCGACTCGCTGCTC GCCTGCGACCAGGACGTCGCCTTCGACGCTCCGGCCGCCGCGTACGACTGGACGAACGTC CGGCATGTGCTCGACGTGGGTGGCGGCAAGGGTGGTTTCGCCGCGGCCATCGCGCGCCGG GCCCCGCACGTGTCGGCCACCGTGCTGGAGATGGCGGGCACCGTGGACACCGCCCGCTCC TACCTGAAGGACGAGGGCCTCTCCGACCGTGTCGACGTCGTCGAGGGGGACTTCTTCGAG CCGCTGCCCCGCAAGGCGGACGCGATCATCCTCTCTTTCGTCCTCCTCAACTGGCCGGAC CACGACGCCGTCCGGATCCTCACCCGCTGCGCCGAGGCCCTGGAGCCCGGCGGGCGCATC CTGATCCACGAGCGCGACGACCTCCACGAGAACTCGTTCAACGAACAGTTCACAGAGCTC GATCTGCGGATGCTGGTCTTCCTCGGCGGTGCCCTGCGCACCCGCGAGAAGTGGGACGGC CTGGCCGCGTCGGCGGGCCTCGTGGTCGAGGAGGTGCGGCAACTGCCGTCGCCGACCATC CCGTACGACCTCTCGCTCCTCGTCCTTGCCCCCGCGGCCACCGGCGCCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q06528 UniProtKB Entry Name DNRK_STRPE GenBank Protein ID 295169 GenBank Gene ID L13453 PDB ID(s) 1TW2, 1TW3, 4WXH KEGG ID ag:AAA26712 - General References
- Madduri K, Torti F, Colombo AL, Hutchinson CR: Cloning and sequencing of a gene encoding carminomycin 4-O-methyltransferase from Streptomyces peucetius and its expression in Escherichia coli. J Bacteriol. 1993 Jun;175(12):3900-4. [Article]
- Madduri K, Hutchinson CR: Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. J Bacteriol. 1995 Jul;177(13):3879-84. [Article]
- Jansson A, Koskiniemi H, Mantsala P, Niemi J, Schneider G: Crystal structure of a ternary complex of DnrK, a methyltransferase in daunorubicin biosynthesis, with bound products. J Biol Chem. 2004 Sep 24;279(39):41149-56. Epub 2004 Jul 24. [Article]
Associated Data
- Bio-Entities
Bio-Entity Type Carminomycin 4-O-methyltransferase DnrK (Streptomyces peucetius) protein primary- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details S-adenosyl-L-homocysteine experimental unknown target Details 4-Methoxy-E-Rhodomycin T experimental unknown target Details