Chloramphenicol 3-O phosphotransferase
Details
- Name
- Chloramphenicol 3-O phosphotransferase
- Synonyms
- 2.7.1.-
- CPT
- Gene Name
- Not Available
- Organism
- Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
- Amino acid sequence
>lcl|BSEQ0021976|Chloramphenicol 3-O phosphotransferase MTTRMIILNGGSSAGKSGIVRCLQSVLPEPWLAFGVDSLIEAMPLKMQSAEGGIEFDADG GVSIGPEFRALEGAWAEGVVAMARAGARIIIDDVFLGGAAAQERWRSFVGDLDVLWVGVR CDGAVAEGRETARGDRVAGMAAKQAYVVHEGVEYDVEVDTTHKESIECAWAIAAHVVP
- Number of residues
- 178
- Molecular Weight
- 18816.255
- Theoretical pI
- 4.38
- GO Classification
- FunctionsATP binding / kinase activityProcessesresponse to antibiotic
- General Function
- Kinase activity
- Specific Function
- Inactivates chloramphenicol by catalyzing the transfer of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl group.
- Pfam Domain Function
- CPT (PF07931)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0002963|537 bp GTGACCACTCGGATGATCATCCTCAACGGCGGTTCCAGCGCGGGGAAGTCCGGCATCGTA CGGTGCCTCCAGTCCGTCCTTCCCGAGCCCTGGCTGGCCTTCGGCGTCGACTCCCTCATC GAGGCGATGCCCCTGAAGATGCAGAGCGCCGAAGGCGGCATCGAGTTCGACGCCGACGGC GGGGTGAGCATCGGGCCCGAGTTCCGCGCCCTTGAGGGCGCCTGGGCCGAGGGCGTCGTC GCGATGGCCCGCGCGGGCGCCCGGATCATCATCGACGACGTCTTCCTCGGCGGTGCCGCC GCCCAGGAGCGCTGGCGGAGCTTCGTCGGGGACCTGGACGTGCTCTGGGTCGGCGTCCGG TGCGACGGCGCCGTCGCCGAGGGCCGGGAGACCGCGCGCGGCGACCGCGTCGCGGGCATG GCGGCGAAGCAGGCGTACGTCGTGCACGAGGGCGTGGAGTACGACGTGGAGGTCGACACC ACGCACAAGGAGTCGATCGAGTGCGCCTGGGCGATCGCCGCCCACGTCGTCCCGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q56148 UniProtKB Entry Name CPT_STRVP GenBank Protein ID 498888 GenBank Gene ID U09991 - General References
- Mosher RH, Camp DJ, Yang K, Brown MP, Shaw WV, Vining LC: Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer. J Biol Chem. 1995 Nov 10;270(45):27000-6. [Article]
- Pullan ST, Chandra G, Bibb MJ, Merrick M: Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes. BMC Genomics. 2011 Apr 4;12:175. doi: 10.1186/1471-2164-12-175. [Article]
- Izard T, Ellis J: The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism. EMBO J. 2000 Jun 1;19(11):2690-700. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB02608 N-[(1R,2R)-1,3-Dihydroxy-1-(4-nitrophenyl)-2-propanyl]acetamide experimental unknown Details DB02930 Adenosine 5'-[gamma-thio]triphosphate experimental unknown Details DB04411 Alpha-N-Dichloroacetyl-P-Aminophenylserinol experimental unknown Details DB00446 Chloramphenicol approved, vet_approved, withdrawn unknown substrate Details