5'-fluoro-5'-deoxy-adenosine synthase
Details
- Name
- 5'-fluoro-5'-deoxy-adenosine synthase
- Synonyms
- 2.5.1.63
- 5'-FDAS
- 5'-fluorodeoxyadenosine synthase
- Fluorinase
- Gene Name
- flA
- Organism
- Streptomyces cattleya
- Amino acid sequence
>lcl|BSEQ0012215|5'-fluoro-5'-deoxy-adenosine synthase MAANSTRRPIIAFMSDLGTTDDSVAQCKGLMYSICPDVTVVDVCHSMTPWDVEEGARYIV DLPRFFPEGTVFATTTYPATGTTTRSVAVRIKQAAKGGARGQWAGSGAGFERAEGSYIYI APNNGLLTTVLEEHGYLEAYEVTSPKVIPEQPEPTFYSREMVAIPSAHLAAGFPLSEVGR PLEDHEIVRFNRPAVEQDGEALVGVVSAIDHPFGNVWTNIHRTDLEKAGIGYGARLRLTL DGVLPFEAPLTPTFADAGEIGNIAIYLNSRGYLSIARNAASLAYPYHLKEGMSARVEAR
- Number of residues
- 299
- Molecular Weight
- 32369.26
- Theoretical pI
- 5.03
- GO Classification
- Functionsadenosyl-fluoride synthase activity
- General Function
- Adenosyl-fluoride synthase activity
- Specific Function
- Involved in the biosynthesis of fluorometabolites. Catalyzes the formation of a C-F bond by combining S-adenosyl-L-methionine (SAM) and fluoride to generate 5'-fluoro-5'-deoxyadenosine (5'-FDA) and L-methionine. It can also use 2'-deoxyadenosine in place of adenosine as substrate.
- Pfam Domain Function
- SAM_adeno_trans (PF01887)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0006179|900 bp ATGGCTGCCAACAGCACACGTCGCCCCATCATCGCGTTCATGAGCGACCTGGGGACCACG GACGACTCCGTCGCCCAGTGCAAGGGGCTCATGTACAGCATCTGCCCGGACGTCACGGTG GTGGACGTCTGCCACTCGATGACGCCCTGGGACGTCGAGGAGGGCGCCCGCTACATCGTG GACCTTCCGCGCTTCTTCCCCGAGGGAACGGTCTTCGCCACCACCACCTATCCGGCGACC GGCACCACCACCCGCTCGGTGGCGGTGCGCATCAAGCAGGCCGCCAAGGGCGGTGCCCGC GGCCAGTGGGCGGGCTCGGGGGCCGGCTTCGAGCGCGCCGAGGGCTCGTACATCTACATC GCGCCCAACAACGGGCTGCTGACCACCGTGCTGGAGGAGCACGGCTACCTGGAGGCGTAC GAGGTCACCTCGCCGAAGGTCATCCCCGAGCAGCCCGAACCGACCTTCTACAGCCGGGAG ATGGTGGCCATCCCCTCCGCGCACCTGGCCGCCGGCTTCCCGCTGTCCGAGGTCGGCCGT CCGCTGGAGGACCACGAGATCGTCCGCTTCAACCGCCCGGCCGTCGAGCAGGACGGGGAG GCGCTGGTGGGCGTGGTCTCCGCCATCGACCACCCGTTCGGCAACGTGTGGACCAACATC CACCGCACCGACCTGGAGAAGGCGGGCATCGGCTACGGCGCCCGGCTGCGGCTGACGCTG GACGGCGTGCTGCCGTTCGAGGCGCCGCTGACCCCGACGTTCGCCGACGCCGGTGAGATC GGCAACATCGCCATCTACCTCAACAGCCGCGGTTACCTGTCCATCGCGCGCAACGCGGCC AGCCTCGCCTACCCGTACCACCTCAAGGAGGGCATGTCCGCCCGGGTCGAGGCCCGCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q70GK9 UniProtKB Entry Name FLA_STRCT GenBank Gene ID AJ581748 - General References
- Dong C, Huang F, Deng H, Schaffrath C, Spencer JB, O'Hagan D, Naismith JH: Crystal structure and mechanism of a bacterial fluorinating enzyme. Nature. 2004 Feb 5;427(6974):561-5. [Article]
- Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB: The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A. Chem Biol. 2006 May;13(5):475-84. [Article]
- Schaffrath C, Deng H, O'Hagan D: Isolation and characterisation of 5'-fluorodeoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya. FEBS Lett. 2003 Jul 17;547(1-3):111-4. [Article]
- Deng H, Cobb SL, McEwan AR, McGlinchey RP, Naismith JH, O'Hagan D, Robinson DA, Spencer JB: The fluorinase from Streptomyces cattleya is also a chlorinase. Angew Chem Int Ed Engl. 2006 Jan 23;45(5):759-62. [Article]
- Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA: Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates. Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. [Article]
- Zhu X, Robinson DA, McEwan AR, O'Hagan D, Naismith JH: Mechanism of enzymatic fluorination in Streptomyces cattleya. J Am Chem Soc. 2007 Nov 28;129(47):14597-604. Epub 2007 Nov 7. [Article]