DNA ligase
Details
- Name
- DNA ligase
- Synonyms
- 6.5.1.2
- Polydeoxyribonucleotide synthase [NAD(+)]
- Gene Name
- ligA
- Organism
- Enterococcus faecalis (strain ATCC 700802 / V583)
- Amino acid sequence
>lcl|BSEQ0016839|DNA ligase MEQQPLTLTAATTRAQELRKQLNQYSHEYYVKDQPSVEDYVYDRLYKELVDIETEFPDLI TPDSPTQRVGGKVLSGFEKAPHDIPMYSLNDGFSKEDIFAFDERVRKAIGKPVAYCCELK IDGLAISLRYENGVFVRGATRGDGTVGENITENLRTVRSVPMRLTEPISVEVRGECYMPK QSFVALNEEREENGQDIFANPRNAAAGSLRQLDTKIVAKRNLNTFLYTVADFGPMKAKTQ FEALEELSAIGFRTNPERQLCQSIDEVWAYIEEYHEKRSTLPYEIDGIVIKVNEFALQDE LGFTVKAPRWAIAYKFPPEEAETVVEDIEWTIGRTGVVTPTAVMAPVRVAGTTVSRASLH NADFIQMKDIRLNDHVIIYKAGDIIPEVAQVLVEKRAADSQPYEMPTHCPICHSELVHLD EEVALRCINPKCPAQIKEGLNHFVSRNAMNIDGLGPRVLAQMYDKGLVKDVADLYFLTEE QLMTLDKIKEKSANNIYTAIQGSKENSVERLIFGLGIRHVGAKAAKILAEHFGDLPTLSR ATAEEIVALDSIGETIADSVVTYFENEEVHELMAELEKAQVNLTYKGLRTEQLAEVESPF KDKTVVLTGKLAQYTREEAKEKIENLGGKVTGSVSKKTDIVVAGEDAGSKLTKAESLGVT VWNEQEMVDALDASHF
- Number of residues
- 676
- Molecular Weight
- 75583.155
- Theoretical pI
- 4.73
- GO Classification
- FunctionsDNA binding / DNA ligase (NAD+) activity / metal ion bindingProcessesDNA repair / DNA replication
- General Function
- Metal ion binding
- Specific Function
- DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0016840|DNA ligase (ligA) ATGGAACAACAACCATTAACATTAACGGCGGCGACAACTCGTGCGCAAGAATTAAGAAAA CAACTCAATCAATATTCCCATGAATATTATGTCAAAGATCAACCATCCGTAGAAGATTAT GTTTACGATCGTTTGTATAAAGAGTTAGTCGATATTGAAACGGAGTTTCCTGATTTAATC ACGCCAGATTCGCCGACACAACGTGTAGGTGGCAAAGTTTTATCTGGTTTTGAAAAAGCT CCGCATGACATTCCAATGTATAGTTTAAATGATGGGTTTAGCAAGGAAGACATTTTTGCT TTTGATGAACGTGTTCGTAAGGCGATTGGTAAACCCGTTGCTTACTGTTGTGAGTTAAAA ATTGATGGGTTAGCAATTTCATTACGTTATGAAAACGGGGTGTTTGTACGCGGAGCAACA CGAGGCGATGGAACGGTTGGTGAAAACATTACAGAGAACTTACGAACGGTTCGCTCTGTA CCGATGCGTTTAACGGAGCCCATTTCAGTGGAAGTTCGTGGCGAGTGCTACATGCCTAAA CAATCCTTTGTCGCATTGAATGAAGAACGTGAAGAAAATGGCCAAGATATTTTTGCCAAT CCTCGGAATGCAGCGGCGGGAAGCTTGCGCCAACTAGATACAAAAATTGTGGCTAAACGG AACTTAAATACGTTTTTATACACAGTTGCTGACTTTGGACCAATGAAAGCGAAGACGCAG TTTGAGGCATTAGAAGAGCTTTCTGCAATTGGTTTTAGAACCAATCCCGAACGGCAATTA TGTCAGTCAATTGATGAAGTTTGGGCCTACATTGAAGAATACCATGAAAAGCGGAGCACT TTGCCATACGAAATCGATGGAATTGTGATTAAAGTGAACGAATTTGCGTTGCAAGATGAA TTAGGTTTCACTGTCAAAGCACCTCGCTGGGCGATTGCCTACAAATTTCCACCAGAAGAA GCAGAAACAGTAGTGGAAGATATTGAATGGACAATTGGGCGCACGGGGGTGGTCACTCCT ACTGCGGTCATGGCTCCTGTACGAGTCGCGGGAACCACGGTTAGTCGGGCAAGTTTGCAT AATGCAGATTTTATTCAAATGAAAGATATCCGTTTAAATGATCATGTTATTATTTACAAA GCCGGCGATATTATCCCAGAAGTAGCGCAAGTGCTAGTTGAAAAACGTGCAGCCGATAGT CAGCCTTATGAAATGCCGACACATTGTCCAATTTGTCATAGTGAGTTGGTGCATTTAGAT GAGGAAGTGGCGTTACGTTGTATTAATCCAAAATGCCCTGCGCAAATTAAAGAAGGGCTG AATCACTTTGTCTCACGAAACGCAATGAACATTGATGGCTTGGGGCCACGAGTGTTAGCA CAAATGTATGACAAAGGATTAGTGAAAGACGTAGCGGATCTGTATTTCTTAACAGAAGAA CAATTAATGACTTTGGATAAAATCAAAGAAAAATCAGCAAACAACATTTATACTGCCATT CAAGGGAGTAAAGAAAACTCTGTCGAACGTTTGATTTTTGGCTTAGGGATTCGCCATGTG GGGGCGAAAGCGGCGAAAATTTTAGCGGAGCATTTTGGTGATTTACCAACCTTAAGTCGT GCAACCGCGGAAGAAATTGTGGCTTTGGATTCAATTGGCGAAACAATCGCAGACAGCGTT GTGACGTATTTTGAAAATGAGGAAGTTCATGAATTAATGGCTGAACTGGAAAAAGCCCAA GTGAACTTGACATATAAAGGTCTCCGGACAGAACAATTAGCAGAAGTGGAGTCACCTTTC AAAGACAAAACAGTTGTTTTAACAGGAAAATTAGCGCAGTACACTCGCGAAGAAGCCAAA GAAAAAATTGAAAATCTTGGTGGAAAAGTTACAGGGAGTGTTTCGAAAAAAACTGACATT GTGGTTGCTGGCGAAGATGCAGGCAGTAAATTAACCAAAGCTGAAAGCTTAGGCGTGACC GTTTGGAATGAACAAGAAATGGTCGATGCGTTAGATGCAAGTCATTTTTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q837V6 UniProtKB Entry Name DNLJ_ENTFA GenBank Gene ID AE016830 - General References
- Paulsen IT, Banerjei L, Myers GS, Nelson KE, Seshadri R, Read TD, Fouts DE, Eisen JA, Gill SR, Heidelberg JF, Tettelin H, Dodson RJ, Umayam L, Brinkac L, Beanan M, Daugherty S, DeBoy RT, Durkin S, Kolonay J, Madupu R, Nelson W, Vamathevan J, Tran B, Upton J, Hansen T, Shetty J, Khouri H, Utterback T, Radune D, Ketchum KA, Dougherty BA, Fraser CM: Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 2003 Mar 28;299(5615):2071-4. [Article]
- Gajiwala KS, Pinko C: Structural rearrangement accompanying NAD+ synthesis within a bacterial DNA ligase crystal. Structure. 2004 Aug;12(8):1449-59. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB03227 Nicotinamide Mononucleotide experimental unknown Details DB07040 2-amino-7-fluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide experimental unknown Details DB07041 N-[2-(2,4-diaminopyrido[2,3-d]pyrimidin-7-yl)-2-methylpropyl]-4-phenoxybenzamide experimental unknown Details DB07042 7-amino-2-tert-butyl-4-{[2-(1H-imidazol-4-yl)ethyl]amino}pyrido[2,3-d]pyrimidine-6-carboxamide experimental unknown Details DB07043 7-amino-2-tert-butyl-4-(4-pyrimidin-2-ylpiperazin-1-yl)pyrido[2,3-d]pyrimidine-6-carboxamide experimental unknown Details