Epsin-1

Details

Name

Epsin-1

Synonyms

• EH domain-binding mitotic phosphoprotein
• EPS-15-interacting protein 1

Gene Name

EPN1

Organism

Humans

Amino acid sequence

>lcl|BSEQ0009373|Epsin-1
MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMI
WKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQG
VNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQS
SGEEELQLQLALAMSKEEADQPPSCGPEDDAQLQLALSLSREEHDKEERIRRGDDLRLQM
AIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPP
VPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPV
SGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAAGGFDTEPDEFSDFDRLRTALPTSGSS
AGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAA
LVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPP
VPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL

Number of residues

576

Molecular Weight

60292.655

Theoretical pI

Not Available

GO Classification

Functions

lipid binding

Processes

embryonic organ development / endocytosis / epidermal growth factor receptor signaling pathway / female pregnancy / in utero embryonic development / negative regulation of epidermal growth factor receptor signaling pathway / Notch signaling pathway

Components

coated pit / cytoplasm / nucleus / plasma membrane / terminal bouton

General Function

Lipid binding

Specific Function

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.

Pfam Domain Function

• ENTH (PF01417)

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0017444|Epsin-1 (EPN1)
ATGGGTGATCAGAGCTGGCTCTGGAACCAGGCTGCTCCAGGAGTAAGGAGCCCAGTCTTT
GCATGCAGTGTTGAAAAAGGTAATGTCCCTCTTGTGCTGAGCGAGCACCTGGCACACAGC
AGGGACCCAGGCAGTGGGGCTGTTAGGTTCCTTATCTCTCCTGAGCCTTGGGCTTCCGCT
ATCCTTGGGACGTCTGGTCTTCTGGCTTCCCCGGTTCTTCCTGCCGCCCTGGATGCGGTG
ACCTGCCAGCACCTGCCGCAGCCTTCGTCCGGGAGTCGCCCCATCTCTCCACGCATCGGG
GCCCTGTGCCCCTTGCTGCTGCAGCCGGGCACCATGTCGACCTCGTCCTTGAGGCGCCAG
ATGAAGAACATCGTCCACAACTACTCAGAGGCGGAGATCAAGGTTCGAGAGGCCACGAGC
AATGACCCCTGGGGCCCATCCAGCTCCCTCATGTCAGAGATTGCCGACCTCACCTACAAC
GTTGTCGCCTTCTCGGAGATCATGAGCATGATCTGGAAGCGGCTCAATGACCATGGCAAG
AACTGGCGTCACGTTTACAAGGCCATGACGCTGATGGAGTACCTCATCAAGACCGGCTCG
GAGCGCGTGTCGCAGCAGTGCAAGGAGAACATGTACGCCGTGCAGACGCTGAAGGACTTC
CAGTACGTGGACCGCGACGGCAAGGACCAGGGCGTGAACGTGCGTGAGAAAGCTAAGCAG
CTGGTGGCCCTGCTGCGCGACGAGGACCGGCTGCGGGAAGAGCGGGCGCACGCGCTCAAG
ACCAAGGAAAAGCTGGCACAGACCGCCACGGCCTCATCAGCAGCTGTGGGCTCAGGCCCC
CCTCCCGAGGCGGAGCAGGCGTGGCCGCAGAGCAGCGGGGAGGAGGAGCTGCAGCTCCAG
CTGGCCCTGGCCATGAGCAAGGAGGAGGCCGACCAGGAGGAGCGGATCCGTCGCGGGGAT
GACCTGCGGCTGCAGATGGCAATCGAGGAGAGCAAGAGGGAGACTGGGGGCAAGGAGGAG
TCGTCCCTCATGGACCTTGCTGACGTCTTCACGGCCCCAGCTCCTGCCCCGACCACAGAC
CCCTGGGGGGGCCCAGCACCCATGGCTGCTGCCGTCCCCACGGCTGCCCCCACCTCGGAC
CCCTGGGGCGGCCCCCCTGTCCCTCCAGCTGCTGATCCCTGGGGAGGTCCAGCCCCCACG
CCGGCCTCTGGGGACCCCTGGAGGCCTGCTGCCCCTGCAGGACCCTCAGTTGACCCTTGG
GGTGGGACCCCAGCCCCTGCAGCTGGGGAGGGGCCCACGCCTGATCCATGGGGAAGTTCC
GATGGTGGGGTCCCGGTCAGTGGGCCCTCAGCCTCCGATCCCTGGACACCGGCCCCGGCC
TTCTCAGATCCCTGGGGAGGGTCACCTGCCAAGCCCAGCACCAATGGCACAACAGCAGCC
GGGGGATTCGACACGGAGCCCGACGAGTTCTCTGACTTTGACCGACTCCGCACGGCACTG
CCGACCTCCGGGAGCAGCGCAGGAGAGCTGGAGCTGCTGGCAGGAGAGGTGCCGGCCCGA
AGCCCTGGGGCGTTTGACATGAGTGGGGTCAGGGGATCTCTGGCTGAGGCTGTGGGCAGC
CCCCCACCTGCAGCCACACCAACTCCCACGCCCCCCACCCGGAAGACGCCGGAGTCATTC
CTGGGGCCCAATGCAGCCCTCGTCGACCTGGACTCGCTGGTGAGCCGGCCGGGCCCCACG
CCGCCTGGAGCCAAGGCCTCCAACCCCTTCCTGCCAGGCGGAGGCCCAGCCACTGGCCCT
TCCGTCACCAACCCCTTCCAGCCCGCGCCTCCCGCGACGCTCACCCTGAACCAGCTCCGT
CTCAGTCCTGTGCCTCCCGTCCCTGGAGCGCCACCCACGTACATCTCTCCCCTTGGCGGG
GGCCCTGGCCTGCCCCCCATGATGCCCCCGGGCCCCCCGGCCCCCAACACTAATCCCTTC
CTCCTATAA

Chromosome Location

19

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	Q9Y6I3
UniProtKB Entry Name	EPN1_HUMAN
HGNC ID	HGNC:21604

General References

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4. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
5. Kariya K, Koyama S, Nakashima S, Oshiro T, Morinaka K, Kikuchi A: Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation. J Biol Chem. 2000 Jun 16;275(24):18399-406. [Article]
6. Rosse C, L'Hoste S, Offner N, Picard A, Camonis J: RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis. J Biol Chem. 2003 Aug 15;278(33):30597-604. Epub 2003 May 29. [Article]
7. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [Article]
8. Edeling MA, Mishra SK, Keyel PA, Steinhauser AL, Collins BM, Roth R, Heuser JE, Owen DJ, Traub LM: Molecular switches involving the AP-2 beta2 appendage regulate endocytic cargo selection and clathrin coat assembly. Dev Cell. 2006 Mar;10(3):329-42. [Article]
9. Beausoleil SA, Villen J, Gerber SA, Rush J, Gygi SP: A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol. 2006 Oct;24(10):1285-92. Epub 2006 Sep 10. [Article]
10. Carrascal M, Ovelleiro D, Casas V, Gay M, Abian J: Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment. J Proteome Res. 2008 Dec;7(12):5167-76. [Article]
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16. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. [Article]
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Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB03316	1,4-Dioxane	experimental	unknown		Details
DB03401	1D-myo-inositol 1,4,5-trisphosphate	experimental	unknown		Details