Orotidine 5'-phosphate decarboxylase

Details

Name
Orotidine 5'-phosphate decarboxylase
Synonyms
  • 4.1.1.23
  • OMP decarboxylase
  • OMPDCase
  • OMPdecase
Gene Name
pyrF
UniProtKB Entry
P25971Swiss-Prot
Organism
Bacillus subtilis (strain 168)
NCBI Taxonomy ID
224308
Amino acid sequence
>lcl|BSEQ0020574|Orotidine 5'-phosphate decarboxylase
MKNNLPIIALDFASAEETLAFLAPFQQEPLFVKVGMELFYQEGPSIVKQLKERNCELFLD
LKLHDIPTTVNKAMKRLASLGVDLVNVHAAGGKKMMQAALEGLEEGTPAGKKRPSLIAVT
QLTSTSEQIMKDELLIEKSLIDTVVHYSKQAEESGLDGVVCSVHEAKAIYQAVSPSFLTV
TPGIRMSEDAANDQVRVATPAIAREKGSSAIVVGRSITKAEDPVKAYKAVRLEWEGIKS
Number of residues
239
Molecular Weight
25991.84
Theoretical pI
6.15
GO Classification
Functions
orotidine-5'-phosphate decarboxylase activity
Processes
'de novo' pyrimidine nucleobase biosynthetic process / 'de novo' UMP biosynthetic process
General Function
Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).
Specific Function
orotidine-5'-phosphate decarboxylase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0020575|Orotidine 5'-phosphate decarboxylase (pyrF)
ATGAAAAACAACCTGCCCATCATCGCGCTTGATTTTGCGTCAGCTGAAGAAACACTTGCG
TTCTTAGCGCCTTTTCAGCAAGAACCGTTATTTGTAAAGGTTGGGATGGAGCTTTTTTAT
CAAGAAGGGCCATCTATCGTGAAACAACTAAAAGAAAGAAACTGCGAGCTATTTTTAGAT
CTAAAGCTTCATGACATCCCGACTACTGTAAACAAAGCGATGAAGCGCCTTGCCAGTCTT
GGAGTAGACCTCGTCAATGTTCATGCTGCCGGGGGCAAAAAAATGATGCAGGCAGCTCTC
GAAGGCTTAGAAGAAGGTACGCCGGCTGGAAAAAAACGTCCGTCACTTATCGCGGTAACC
CAGCTGACAAGCACATCTGAACAAATCATGAAAGATGAACTGCTGATCGAAAAGTCTCTG
ATTGATACGGTTGTGCACTACAGCAAACAGGCGGAAGAAAGCGGACTGGATGGAGTGGTC
TGCTCTGTTCATGAAGCAAAAGCCATTTACCAAGCGGTGTCGCCTTCATTTCTGACTGTC
ACTCCGGGGATCAGAATGTCAGAGGACGCTGCGAATGACCAAGTTCGCGTAGCGACGCCT
GCCATTGCAAGAGAGAAAGGTTCATCAGCGATTGTAGTAGGACGCTCGATTACAAAAGCG
GAAGACCCGGTAAAAGCCTATAAGGCTGTCAGACTTGAATGGGAGGGAATCAAATCTTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP25971
UniProtKB Entry NamePYRF_BACSU
GenBank Protein ID143392
GenBank Gene IDM59757
PDB ID(s)1DBT
KEGG IDbsu:BSU15550
NCBI Gene ID935960
General References
  1. Quinn CL, Stephenson BT, Switzer RL: Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon. J Biol Chem. 1991 May 15;266(14):9113-27. [Article]
  2. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
  3. Appleby TC, Kinsland C, Begley TP, Ealick SE: The crystal structure and mechanism of orotidine 5'-monophosphate decarboxylase. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2005-10. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Orotidine 5'-phosphate decarboxylase (Bacillus subtilis (strain 168))protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Uridine monophosphateexperimentalunknowntargetDetails