[The reaction mechanism between cefoperazone and human serum albumin].

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Liu LS, Zhang YY, Wang XP

[The reaction mechanism between cefoperazone and human serum albumin].

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Sep;25(9):1490-2.

PubMed ID

16379298 [ View in PubMed

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Abstract

The reaction mechanism between cefoperazone and human serum albumin (HSA) and the affinity between cefoperazone and beta-lactamase were investigated by spectrometry and spectrofluorimetry. The interaction dissociation constants of human serum albumin and cefoperazone have been determined from a double reciprocal Lineweaver-Burk plot. The binding distance and the transfer efficiency between cefoperazone and HSA were also obtained according to the theory of Forster' non-radiation energy transfer. The result suggested that the main binding force between cefoperazone and HSA is electrostatic force interaction. The high beta-lactamase stability of cefoperazone may be correlative with its molecular structure. The antibiotic activity and valency connect with transfer efficiency and dissociation constant. The effect of cefoperazone on the conformation of HSA was also analyzed using synchronous fluorescence spectrometry.

DrugBank Data that Cites this Article

Drug Carriers

Drug	Carrier	Kind	Organism	Pharmacological Action	Actions
Cefoperazone	Serum albumin	Protein	Humans	No	Other/unknown	Details