[Transcriptional regulation of placental transforming growth factor-beta by calcitriol in prostate cancer cells is androgen-independent].
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Nazarova NIu, Chikhirzhina GI, Tuohimaaa P
[Transcriptional regulation of placental transforming growth factor-beta by calcitriol in prostate cancer cells is androgen-independent].
Mol Biol (Mosk). 2006 Jan-Feb;40(1):84-9.
- PubMed ID
- 16523695 [ View in PubMed]
- Abstract
Calcitriol (1 alpha,25-dihydroxycholecalciferol) inhibits prostate cancer cell growth. It has been shown that inhibition of growth of prostate cancer LNCaP cells by calcitriol is androgen-dependent. Using cDNA microarray we showed that calcitriol induced expression of placental transforming growth factor-beta (PTGF-beta), which is known to suppress cell growth. We studied regulation of PTGF-beta gene expression by calcitriol and 5alpha-dihydrotestosterone and analyzed whether induction of PTGF-beta transcription by calcitriol is androgen-dependent. Using real-time PCR we demonstrate that 5alpha-dihydrotestosterone up-regulates PTGF-beta mRNA. We do not find an effect of 5alpha-dihydrotestosterone or antiandrogen Casodex on calcitriol-induced PTGF-beta mRNA level and conclude that induction of PTGF-beta transcription by calcitriol is androgen-independent.