Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells.
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Fukada K, Takahashi-Yanaga F, Sakoguchi-Okada N, Shiraishi F, Miwa Y, Morimoto S, Sasaguri T
Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells.
Biochem Biophys Res Commun. 2007 Jun 15;357(4):1166-71. Epub 2007 Apr 19.
- PubMed ID
- 17466271 [ View in PubMed]
- Abstract
The effect of celecoxib, a cyclooxygenase-2 selective inhibitor, on a human cervical cancer cell line, HeLa cells, was examined. We found that celecoxib increased DNA ladder formation and the activity of caspase-3, indicating that celecoxib induced apoptosis in HeLa cells. Celecoxib suppressed the expression of an anti-apoptotic protein, survivin, in both protein and mRNA levels. The overexpression of survivin overrode caspase-3 activation induced by celecoxib. Subsequently, we performed luciferase reporter assay with the reporter vector containing human survivin promoter region and electrophoretic mobility shift assay and found that the -75 to -66 bp region relative to the initiating codon played an important role in celecoxib action to suppress survivin promoter activity. Our findings might provide a new insight into the anti-cancer effects of celecoxib.