Effects of 1-bromopropane, a substitute for chlorofluorocarbons, on BDNF expression.

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Yoshida Y, Nakano Y, Ueno S, Liu J, Fueta Y, Ishidao T, Kunugita N, Yanagihara N, Sugiura T, Hori H, Yamashita U

Effects of 1-bromopropane, a substitute for chlorofluorocarbons, on BDNF expression.

Int Immunopharmacol. 2009 Apr;9(4):433-8. doi: 10.1016/j.intimp.2009.01.007. Epub 2009 Feb 2.

PubMed ID

19189864 [ View in PubMed

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Abstract

1-Bromopropane (1-BP) has been widely used as an alternative to ozone-depleting chlorofluorocarbons in various industries. Although the neurotoxicity of 1-BP has been recently reported, there is little information about the effect of 1-BP on the cells in brain by experimental approach. Here we studied the effect of 1-BP on brain-derived neurotrophic factor (BDNF) expression in astrocytes in vitro. The BDNF mRNA level was remarkably decreased by 1-BP in a human astrocytoma cell line, U251, and in mouse primary astrocytes. The DNA-binding and specific reporter activity of cAMP response element-binding transcription factor (CREB), which is one of the key molecules regulating BDNF expression, were reduced by 1-BP in U251 and/or mouse primary astrocytes. Additionally, protein kinase A (PKA) activity was suppressed by 1-BP in U251. These results suggest that BDNF expression was affected by 1-BP through at least PKA.