Effects of copper on the expression of metal transporters in human intestinal Caco-2 cells.
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Tennant J, Stansfield M, Yamaji S, Srai SK, Sharp P
Effects of copper on the expression of metal transporters in human intestinal Caco-2 cells.
FEBS Lett. 2002 Sep 11;527(1-3):239-44.
- PubMed ID
- 12220667 [ View in PubMed]
- Abstract
Copper is an essential dietary trace metal, however the mechanisms involved in intestinal copper uptake are unclear. Two putative copper transporters are expressed in Caco-2 cells, the divalent metal transporter (DMT1) and copper transporter (Ctr1). Our data demonstrate that copper could compete with iron for uptake via DMT1 and that DMT1 protein and mRNA expression were decreased following exposure (24 h) to high copper. Expression of Ctr1, which acts as a copper transporter in transfected cell lines, was unaffected by copper treatment. Interestingly, exposure to copper increased iron efflux from Caco-2 cells and up regulated IREG1 (iron-regulated mRNA) expression.