The genomic response of a human uterine endometrial adenocarcinoma cell line to 17alpha-ethynyl estradiol.
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Naciff JM, Khambatta ZS, Thomason RG, Carr GJ, Tiesman JP, Singleton DW, Khan SA, Daston GP
The genomic response of a human uterine endometrial adenocarcinoma cell line to 17alpha-ethynyl estradiol.
Toxicol Sci. 2009 Jan;107(1):40-55. doi: 10.1093/toxsci/kfn219. Epub 2008 Oct 20.
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- 18936297 [ View in PubMed]
- Abstract
We have determined the gene expression profile induced by 17 alpha-ethynyl estradiol (EE) in Ishikawa cells, a human uterine-derived estrogen-sensitive cell line, at various doses (1 pM, 100 pM, 10 nM, and 1 microM) and time points (8, 24, and 48 h). The transcript profiles were compared between treatment groups and controls (vehicle-treated) using high-density oligonucleotide arrays to determine the expression level of approximately 38,500 human genes. By trend analysis, we determined that the expression of 2560 genes was modified by exposure to EE in a dose- and time-dependent manner (p