[Effect of nylestriol and levonorgestrel on the expression of Opg/OPGL in human osteosarcoma MG-63 cell lines].
Article Details
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Yang KC, Liao EY, Zhou HD, Xiao XH
[Effect of nylestriol and levonorgestrel on the expression of Opg/OPGL in human osteosarcoma MG-63 cell lines].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Dec;29(6):667-70.
- PubMed ID
- 16114553 [ View in PubMed]
- Abstract
OBJECTIVE: To determine the effect of nylestriol and levonorgestrel on mRNA expression of OPG/OPGL in MG-63 cell lines, and to explore whether the paracrine effect is involved in the regulation course. Methods Semi-quantitive RT-PCR was conducted to analyze mRNA expression of OPG/OPGL in MG-63 cell lines treated with nylestriol and levonorgestrel. Results Both medicines could stimulate MG-63 cell lines to express OPG, and the best action levels of the two medicines were 10(-6) mol/L. Both medicines could decrease mRNA expression levels of OPGL dramatically. In NYL 10(-10) mol/L treatment group, medium renewal has negative effect on mRNA expression of OPG. Conclusion Both the two medicines can promote mRNA expression of OPG in MG-63 cell lines, while decrease mRNA expression of OPGL. The paracrine effect of MG-63 cell lines may be involved in the regulation of mRNA expression of OPG by NYL.