Induction of CYP1A1 and CYP1A2 expressions by prototypic and atypical inducers in the human lung.
Article Details
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Wei C, Caccavale RJ, Weyand EH, Chen S, Iba MM
Induction of CYP1A1 and CYP1A2 expressions by prototypic and atypical inducers in the human lung.
Cancer Lett. 2002 Apr 8;178(1):25-36.
- PubMed ID
- 11849738 [ View in PubMed]
- Abstract
The inducibility of cytochrome P4501A1 gene (CYP1A1) expression was examined in human lung samples from 27 subjects, using an explant culture system and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. CYP1A1 transcripts were present in all of the lung specimens and were induced by the prototypic inducers 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and benzo[a]pyrene (B[a]P), and by the atypical inducers pyridine, nicotine, and omeprazole. 2-Hydroxypyridine was a better inducer than pyridine, implicating metabolites in CYP1A1 induction by the parent compound. The prototypical inducers were the most effective inducers in many samples but were ineffective in some samples in which the atypical compounds were effective inducers. Cytochrome P4501A2 (CYP1A2) transcripts were also detected in most of the lung specimens and were inducible in some specimens. The results show the suitability of the explant culture system for examining the inducibility of human pulmonary CYP1A1 and CYP1A2, indicate the heterogeneity in individual sensitivity to the induction, and underscore the need to include atypical inducers in studies of CYP1A inducibility in humans.
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Nicotine Cytochrome P450 1A1 Protein Humans UnknownSubstrateInducerDetails - Pharmaco-transcriptomics
Drug Drug Groups Gene Gene ID Change Interaction Chromosome Omeprazole Approved Investigational Vet Approved CYP1A1 1543 upregulated Omeprazole results in increased expression of CYP1A1 mRNA 15q24.1