Induction of CYP1A1 and CYP1A2 expressions by prototypic and atypical inducers in the human lung.

Article Details

Citation

Wei C, Caccavale RJ, Weyand EH, Chen S, Iba MM

Induction of CYP1A1 and CYP1A2 expressions by prototypic and atypical inducers in the human lung.

Cancer Lett. 2002 Apr 8;178(1):25-36.

PubMed ID
11849738 [ View in PubMed
]
Abstract

The inducibility of cytochrome P4501A1 gene (CYP1A1) expression was examined in human lung samples from 27 subjects, using an explant culture system and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. CYP1A1 transcripts were present in all of the lung specimens and were induced by the prototypic inducers 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and benzo[a]pyrene (B[a]P), and by the atypical inducers pyridine, nicotine, and omeprazole. 2-Hydroxypyridine was a better inducer than pyridine, implicating metabolites in CYP1A1 induction by the parent compound. The prototypical inducers were the most effective inducers in many samples but were ineffective in some samples in which the atypical compounds were effective inducers. Cytochrome P4501A2 (CYP1A2) transcripts were also detected in most of the lung specimens and were inducible in some specimens. The results show the suitability of the explant culture system for examining the inducibility of human pulmonary CYP1A1 and CYP1A2, indicate the heterogeneity in individual sensitivity to the induction, and underscore the need to include atypical inducers in studies of CYP1A inducibility in humans.

DrugBank Data that Cites this Article

Drug Enzymes
DrugEnzymeKindOrganismPharmacological ActionActions
NicotineCytochrome P450 1A1ProteinHumans
Unknown
Substrate
Inducer
Details
Pharmaco-transcriptomics
DrugDrug GroupsGeneGene IDChangeInteractionChromosome
OmeprazoleApproved Investigational Vet ApprovedCYP1A11543
upregulated
Omeprazole results in increased expression of CYP1A1 mRNA15q24.1