Periostin mediates the increased pro-angiogenic activity of gastric cancer cells under hypoxic conditions.

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Qiu F, Shi CH, Zheng J, Liu YB

Periostin mediates the increased pro-angiogenic activity of gastric cancer cells under hypoxic conditions.

J Biochem Mol Toxicol. 2013 Jul;27(7):364-9. doi: 10.1002/jbt.21498. Epub 2013 May 31.

PubMed ID

23728938 [ View in PubMed

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Abstract

This study was conducted to investigate the biological role of periostin in gastric cancer (GC) under hypoxia. Western blot analysis revealed that along with an upregulation of hypoxia-inducible factor-1alpha, there was a time-dependent induction of periostin in MKN-45 cells under hypoxia (2% O2 ), increasing by eightfold as compared to normoxic cells. Pretreatment with 30 microM PD98059, an inhibitor of ERK1/2, significantly reduced hypoxia-stimulated periostin expression (P < 0.01). Periostin knockdown in MKN-45 cells was achieved by specific small interfering RNA (siRNA). The conditioned medium from periostin siRNA-transfected MKN-45 cells induced significantly less (P < 0.01) endothelial tube formation than control siRNA-transfected cells. Additionally, periostin silencing markedly decreased the mRNA expression and secretion of vascular endothelial growth factor (VEGF) in hypoxic MKN-45 cells. Thus, our data suggest that periostin is a hypoxia-response gene and mediates a cross talk between GC and endothelial cells under hypoxia, partially through regulation of the VEGF expression.