Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells.

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Castagna R, Davis PA, Vasu VT, Soucek K, Cross CE, Greci L, Valacchi G

Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells.

Toxicol In Vitro. 2009 Apr;23(3):365-70. doi: 10.1016/j.tiv.2008.12.016. Epub 2008 Dec 30.

PubMed ID

19162161 [ View in PubMed

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Abstract

Exposure to high levels of ozone (O(3)) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O(3)-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O(3)-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O(3) and/or O(3)-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O(3) and/or its cytotoxic products and may provide protections against O(3)-induced biotoxicities.