Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis.
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Vock C, Gleissner M, Klapper M, Doring F
Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis.
Biochim Biophys Acta. 2007 Sep;1770(9):1283-8. Epub 2007 Jul 10.
- PubMed ID
- 17658692 [ View in PubMed]
- Abstract
Palmitate is the most abundant saturated fatty acid in the human diet and the major one synthesized de novo. To identify palmitate-regulated genes we performed whole genome mRNA expression profiling by using human hepatoma HepG2 cells. We identified eleven genes which are significantly (single-sided permutational t-test, p<0.05) regulated by low concentration of palmitate (50 microM). We observed a decreased expression of five metallothioneins, and an increased expression of liver expressed plasminogen activator inhibitor-1 protein and insulin-like growth factor II, which play a prominent role in the development of the metabolic syndrome. Comparative promoter analysis in-silico revealed common transcriptional regulation of differentially expressed genes through erythroid kruppel-like factor and members of the zinc binding protein factor family. In conclusion, low physiological palmitate concentrations changed expression of very responsive genes.