[Effect of quercetin on glioma cell U87 apoptosis and feedback regulation of MDM2-p53].
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Wang H, Yuan Z, Chen Z, Yao F, Hu Z, Wu B
[Effect of quercetin on glioma cell U87 apoptosis and feedback regulation of MDM2-p53].
Nan Fang Yi Ke Da Xue Xue Bao. 2014 May;34(5):686-9.
- PubMed ID
- 24849437 [ View in PubMed]
- Abstract
OBJECTIVE: To investigate the effect of quercetin on apoptosis and feedback regulation of MDM2-p53 in multiform glioblastoma U87 cells in vitro. METHODS: U87 cells exposed to different concentrations of quercetin (50, 100, and 150 micromol/L) were examined with flow cytometry, RT-PCR and Western blotting for detecting the cell apoptosis, MDM2 mRNA expression, and p53 and caspase-3 expressions. RESULTS: Quercetin induced obvious apoptosis in U87 cells in a concentration-dependent manner, with apoptosis rates of (12.40-/+0.70)% at Q0, (22.53-/+0.72)% at Q50, (29.06-/+0.81)% at Q100, and (31.5-/+0.45)% at Q150. Quercetin significantly increased the expressions of MDM2 mRNA and active caspase-3 protein but decreased the expression of p53 in the cells. CONCLUSION: Quercetin promotes the apoptosis of multiform glioblastoma U87 cells mediated by caspase-3 and influences the feedback balance of MDM2-p53.