Resveratrol induces apoptosis of bladder cancer cells via miR21 regulation of the Akt/Bcl2 signaling pathway.
Article Details
- CitationCopy to clipboard
Zhou C, Ding J, Wu Y
Resveratrol induces apoptosis of bladder cancer cells via miR21 regulation of the Akt/Bcl2 signaling pathway.
Mol Med Rep. 2014 Apr;9(4):1467-73. doi: 10.3892/mmr.2014.1950. Epub 2014 Feb 13.
- PubMed ID
- 24535223 [ View in PubMed]
- Abstract
Resveratrol, an edible polyphenolic phytoalexin present in grapes and red wine, has been reported to inhibit proliferation and induce apoptosis of bladder cancer cells. In the present study, the molecular mechanisms of resveratrol on human bladder cancer cell apoptosis was examined. The effect of resveratrol on the viability and apoptosis of T24 and 5637 cells was measured using an MTT assay and flow cytometric analysis, respectively. Next, the effect of resveratrol on miR21 expression was detected by realtime PCR. The expression of phosphoAkt and Bcl2 following treatment with resveratrol or the downregulation of miR21 expression were also measured. Resveratrol induced the cytotoxicity and apoptosis of T24 and 5637 cells in a dosedependent manner. Resveratrol decreased the expression of miR21, the level of phosphoAkt and Bcl2 protein expression. In addition, the downregulation of miR21 expression inhibited the level of phosphoAkt and Bcl2 expression. Insulinlike growth factor1 was able to reverse the effect of the miR21 inhibitor on Bcl2 expression and apoptosis in T24 and 5637 cells. Notably, overexpression of miR21 expression was able to restore the inhibition of Akt activity, downregulation of Bcl2 expression and apoptosis induced by resveratrol. Collectively, data revealed that the effect of resveratrol on bladder cancer cell apoptosis was due to miR21 regulation of the Akt/Bcl2 signaling pathway.