Resveratrol reduces TNF-alpha-induced U373MG human glioma cell invasion through regulating NF-kappaB activation and uPA/uPAR expression.
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Ryu J, Ku BM, Lee YK, Jeong JY, Kang S, Choi J, Yang Y, Lee DH, Roh GS, Kim HJ, Cho GJ, Choi WS, Kim N, Kang SS
Resveratrol reduces TNF-alpha-induced U373MG human glioma cell invasion through regulating NF-kappaB activation and uPA/uPAR expression.
Anticancer Res. 2011 Dec;31(12):4223-30.
- PubMed ID
- 22199285 [ View in PubMed]
- Abstract
BACKGROUND: High invasiveness of glioma cells is one of the reasons that patients with malignant glioma have a poor prognosis. Resveratrol, a plant compound abundant in the peel of grapes, has been suggested as a potential cancer chemopreventive agent. Therefore, we investigated the effect of resveratrol on glioma cell invasion. MATERIALS AND METHODS: The effect of resveratrol on U373MG human glioma cell invasion was assessed by Matrigel assay and methylthiazoltetrazolium assay. Western blotting and reverse transcription-polymerase chain reaction were performed to elucidate the action mechanism of resveratrol. RESULTS: Resveratrol reduced tumor necrosis factor (TNF)-alpha-induced U373MG human glioma cell invasion. In addition, resveratrol repressed nuclear factor kappa B (NF-kappaB) activation and down-regulated mRNA expression of urokinase plasminogen activator (uPA) and its receptor in TNF-alpha-treated glioma cells. CONCLUSION: These findings suggest that resveratrol could prevent glioma cell invasion via inhibiting proteolysis of extracellular matrix.