Transcriptional responses of human aortic endothelial cells to nanoconstructs used in biomedical applications.
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Moos PJ, Honeggar M, Malugin A, Herd H, Thiagarajan G, Ghandehari H
Transcriptional responses of human aortic endothelial cells to nanoconstructs used in biomedical applications.
Mol Pharm. 2013 Aug 5;10(8):3242-52. doi: 10.1021/mp400285u. Epub 2013 Jul 10.
- PubMed ID
- 23806026 [ View in PubMed]
- Abstract
Understanding the potential toxicities of manufactured nanoconstructs used for drug delivery and biomedical applications may help improve their safety. We sought to determine if surface-modified silica nanoparticles and poly(amido amine) dendrimers elicit genotoxic responses on vascular endothelial cells. The nanoconstructs utilized in this study had a distinct geometry (spheres vs worms) and surface charge, which were used to evaluate the contributions of these parameters to any potential adverse effects of these materials. Time-dependent cytotoxicity was found for surfaced-functionalized but geometrically distinct silica materials, while amine-terminated dendrimers displayed time-independent cytotoxicity and carboxylated dendrimers were nontoxic in our assays. Transcriptomic evaluation of human aortic endothelial cell (HAEC) responses indicated time-dependent gene induction following silica exposure, consisting of cell cycle gene repression and pro-inflammatory gene induction. However, the dendrimers did not induce genomic toxicity, despite displaying general cytotoxicity.